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RISS 인기검색어
- 검색키워드
- 저자 : David R. Nelson (검색결과 3 건)
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Siyi Liu,David R. Nelson,Jing Zhao,Hongxia Hua,Yueping He 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.2
The mirid bug, Cyrtorhinus lividipennis Reuter, an important predatory natural enemy of rice planthoppers, is widely distributed in rice fields. However, genetic information on C. lividipennis is lacking. Especially, limited data about mechanisms of insecticide selectivity between this piercing-sucking predator (C. lividipennis) and piercing-sucking preys (rice planthoppers), inhibits development of selective insecticides and the integration of chemical and biological control systems to control insect pests of rice. Hence, we performed de novo assembly of a transcriptome from adult and nymph whole bodies of C. lividipennis. A total of> 29 million of reads were generated, and 34,752 transcripts matched known proteins. Then, the genes related to insecticide action and detoxification were manually identified, including 26 carboxylesterases (containing 2 acetylcholinesterases), 57 cytochrome P450s, 19 glutathione S-transferases, 15 nicotinic acetylcholine receptors, 3 GABA-gated ion channels, and 1 glutamate receptor. Comparisons of sequence differences in these genes between C. lividipennis and rice planthoppers, revealed that quite a lot of diversity was found among genes related to insecticide action and detoxification, while a few of these genes share much higher identities between this predator and prey. The present study provides useful information for our understanding of insecticide selectivity between rice planthoppers and the predator mirid bug.
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Han, Jeonghoon,Won, Eun-Ji,Kim, Hui-Su,Nelson, David R.,Lee, Su-Jae,Park, Heum Gi,Lee, Jae-Seong American Chemical Society 2015 Environmental science & technology Vol.49 No.11
<P>The 46 cytochrome P450 (CYP) gene superfamily was identified in the marine copepod Paracyclopina nana after searching an RNA-seq database and comparing it with other copepod CYP gene families. To annotate the 46 Pn-CYP genes, a phylogenetic analysis of CYP genes was performed using a Bayesian method. Pn-CYP genes were separated into five different clans: CYP2, CYP3, CYP20, CYP26, and mitochondrial. Among these, the principal Pn-CYP genes involved in detoxification were identified by comparing them with those of the copepod Tigriopus japonicus and were examined with respect to their responses to exposure to a water-accommodated fraction (WAF) of crude oil and to the alkylated forms of two polycyclic aromatic hydrocarbons (PAHs; phenanthrene and fluorene). The expression of two Pn-CYP3027 genes (CYP3027F1 and CYP3027F2) was increased in response to WAF exposure and also was upregulated in response to the two alkylated PAHs. In particular, Pn-CYP3027F2 showed the most notable increase in response to 80% WAF exposure. These two responsive CYP genes (Pn-CYP3027F1 and CYP3027F2) were also phylogenetically clustered into the same clade of the WAF- and alkylated PAH-specific CYP genes of the copepod T. japonicus, suggesting that these CYP genes would be those chiefly involved in detoxification in response to WAF exposure in copepods. In this paper, we provide information on the copepod P. nana CYP gene superfamily and also speculate on its potential role in the detoxification of PAHs in marine copepods. Despite the nonlethality of WAF, Pn-CYP3027F2 was rapidly and significantly upregulated in response to WAF that may serve as a useful biomarker of 40% or higher concentration of WAF exposure. This paper will be helpful to better understand the molecular mechanistic events underlying the metabolism of environmental toxicants in copepods.</P>
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Kim, Ryeo-Ok,Kim, Bo-Mi,Jeong, Chang-Bum,Nelson, David R.,Lee, Jae-Seong,Rhee, Jae-Sung American Chemical Society 2013 Environmental science & technology Vol.47 No.23
<P>Cytochrome P450 (<I>CYP</I>) proteins are involved in the first line of detoxification mechanism against diverse polycyclic aromatic hydrocarbons (PAHs) including benzo[<I>a</I>]pyrene (B[<I>a</I>]P). In aquatic invertebrates, there is still a lack of knowledge on the <I>CYP</I> genes involved in the molecular response to B[<I>a</I>]P exposure due to limited gene information. In this study, we cloned the entire 25 <I>CYP</I> genes in the monogonont rotifer Brachionus koreanus with the aid of next generation sequencing (NGS) technologies and analyzed their transcript profiles with a real-time RT-PCR array to better understand B[<I>a</I>]P-triggered molecular response over different time courses. As a result, B[<I>a</I>]P exposure induced <I>CYP2</I>/<I>3</I>-involved detoxification mechanisms and defensome, including phase II detoxification and antioxidant systems with a modulation of the chaperone heat shock protein (<I>hsp</I>) expression but did not change expression of other <I>CYP</I> clans in B. koreanus. Therefore, we found that B[<I>a</I>]P induced a strong detoxification mechanism to overcome detrimental effects of B[<I>a</I>]P associated with B[<I>a</I>]P-induced growth retardation as a trade-off in fitness costs. Also, this approach revealed that the entire <I>CYP</I> profiling can be a way of providing a better understanding on the mode of action of B[<I>a</I>]P in B. koreanus with respect to molecular defense metabolism.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/esthag/2013/esthag.2013.47.issue-23/es403269v/production/images/medium/es-2013-03269v_0007.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/es403269v'>ACS Electronic Supporting Info</A></P>
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