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Paeoniflorin reduces the inflammatory response of THP-1 cells by up‐regulating microRNA-124
Danyun Huang,Zhijun Li,Yue Chen,Yan Fan,Tao Yu 한국유전학회 2021 Genes & Genomics Vol.43 No.6
Background The activation of macrophages and the release of infammatory cytokines are the main reasons for the progress of systemic lupus erythematosus (SLE). MicroRNA (miRNA)-124 is involved in the regulation of macrophages and is a key regulator of infammation and immunity. Objective To explore whether paeoniforin (PF) regulates the biological functions of macrophages depends on miR-124. Methods RT-PCR, WB, ELISA, CCK-8 and fow cytometry were used to evaluate that PF regulated the biological functions of THP-1 cells through miR-124. Results PF signifcantly inhibited the proliferation while promotes the apoptosis of THP-1 cells, and inhibited the release of IL-6, TNF-α and IL-1βin THP-1 cells. RT-PCR results shown that PF up-regulated the expression of miR-124 in THP-1 cells. Functional recovery experiments showed that compared with the LPS+mimic-NC group, LPS+miR-124 mimic signifcantly inhibited the proliferation and the release of IL-6, TNF-α and IL-1β, but promoted the apoptosis of THP-1 cells. In addition, compared with the LPS+PF+inhibitor-NC group, LPS+PF+miR-124 inhibitor signifcantly promoted the proliferation and the release of IL-6, TNF-α and IL-1β, but inhibited the apoptosis of THP-1 cells. Conclusions By down-regulating miR-124, PF inhibits the proliferation and infammation of THP-1 cells, and promotes the apoptosis of THP-1 cells.
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