http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Daniel Tinôco,Lucy Seldin,Paulo Luiz de Andrade Coutinho,Denise Maria Guimarães Freire 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.125 No.-
Optimization of culture conditions can be used as an alternative metabolic control strategy to geneticengineering to improve bio-based 2,3-butanediol (2,3-BDO) production, especially its yield and opticalpurity. In this study, a first-order model was determined to predict the 2,3-BDO yield, which reachedabout 90% in batch cultures at 32 C, pH = 5, and kLa 5.0 ± 2.7 h1, using a new and safe Paenibacilluspeoriae NRRL BD-62. No acetoin accumulation and an absolute levo-2,3-BDO optical purity were also verified. About 39.4 g/L of 2,3-BDO, with a yield of 0.43 g/g, and a levo-:meso-2,3-BDO ratio of 1.9:1, wasobtained at the expense of acetoin, lactic acid, and ethanol in fed-batch fermentation at kLa adjustedwithin its optimized range (7.5 h1) to overcome operational limitations using high initial glucose concentrations. The low pH contributed to the carbon flux shift toward the levo-isomer synthesis, possiblydue to the preferential butanediol dehydrogenase activation, while the highly oxygen-limited environmentfavored the complete acetoin conversion, enhancing the 2,3-BDO yield. These results provide significantcontributions to the high-yield, acetoin-free, and safe bio-based 2,3-BDO production ofparticular interest in large-scale fermentations.