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Metal Leaching from Spent Petroleum Catalyst by Acidophilic Bacteria in Presence of Pyrite
Kim, Dong J.,Mishra, D.,Park, K. H.,Ahn, J. G.,Ralph, D. E. The Japan Institute of Metals 2008 MATERIALS TRANSACTIONS Vol.49 No.10
<P>This paper describes studies on the recovery of metals from spent hydro-processing catalyst using mixed acidophilic culture in presence of pyrite. This culture was initially grown in the 9K<SUP>−</SUP> medium (absence of 9 g/L Fe(II)) where ferrous sulphate (FeSO<SUB>4</SUB>) was replaced by pyrite, and then applied in this bioleaching study. Bacterial action on pyrite catalysed the formation of ferric ion (Fe<SUP>+3</SUP>), proton (H<SUP>+</SUP>) and sulphate ions (SO<SUB>4</SUB><SUP>−2</SUP>) in the solution which leached metals (Ni, Mo and V) from the spent catalyst. Experiments were conducted by varying the reaction time, amount of spent catalyst and pyrite, and temperature. After 7 days with 30 g/L of spent catalyst and 50 g/L of pyrite, the leaching of Ni, V and Mo into the solution was 85, 92 and 26%, respectively. With increasing spent catalyst loading, the extent of metal dissolution was decreased, probably due to the precipitation of Fe<SUP>+3</SUP> as a residue. Under all conditions tested, Mo showed recovery due to its precipitation with leach residues as MoO<SUB>3</SUB> observed by applying EDAX and XRD techniques to the leach residues.</P>
Human Somatic Cell Nuclear Transfer Using Adult Cells
Chung, Y.,Eum, J.,Lee, J.,Shim, S.,Sepilian, V.,Hong, S.,Lee, Y.,Treff, Nathan R.,Choi, Y.,Kimbrel, Erin A.,Dittman, Ralph E.,Lanza, R.,Lee, D. Cell Press 2014 Cell stem cell Vol.14 No.6
Derivation of patient-specific human pluripotent stem cells via somatic cell nuclear transfer (SCNT) has the potential for applications in a range of therapeutic contexts. However, successful SCNT with human cells has proved challenging to achieve, and thus far has only been reported with fetal or infant somatic cells. In this study, we describe the application of a recently developed methodology for the generation of human ESCs via SCNT using dermal fibroblasts from 35- and 75-year-old males. Our study therefore demonstrates the applicability of SCNT for adult human cells and supports further investigation of SCNT as a strategy for regenerative medicine.