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Daisy Pérez-Brito,Anuar Magaña-Alvarez,Patricia Lappe-Oliveras,Alberto Cortes-Velazquez,Claudia Torres-Calzada,Teófilo Herrera- Suarez,Alfonso Larqué-Saavedra,Raul Tapia-Tussell 한국미생물학회 2015 The journal of microbiology Vol.53 No.1
This study characterized Clavispora lusitaniae strains isolatedfrom different stages of the processing and early fermentationof a henequen (Agave fourcroydes) spirit produced inYucatan, Mexico using a molecular technique. Sixteen strainsidentified based on morphological features, obtained fromdifferent substrates, were typed molecularly. Nine differentversions of the divergent D1/D2 domain of the large-subunitribosomal DNA sequence were identified among theC. lusitaniae strains. The greatest degree of polymorphismwas found in the 90-bp structural motif of the D2 domain. The MSP-PCR technique was able to differentiate 100% ofthe isolates. This study provides significant insight into thegenetic diversity of the mycobiota present during the henequenfermentation process, especially that of C. lusitaniae,for which only a few studies in plants have been published. The applied MSP-PCR markers were very efficient in revealingpolymorphisms between isolates of this species.