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- 저자 : Chuanha Wei (검색결과 2 건)
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Protein Profiles of Major Korean Rice Cultivars
Wei, Chuanha,Kwon, Oh-Yun,Liu, Xi-Wen,Kim, Hyoung-Chin,Yoon, Won-Kee,Kim, Hwan-Mook,Kim, Mee-Ree The Korean Society of Food Science and Nutrition 2007 Preventive Nutrition and Food Science Vol.12 No.2
The protein profiles among Korean rice cultivars were assessed by total protein determination, solubility fractionation, SDS-PAGE analysis and scanning densitometry. In the extraction of protein, the SDS/urea system at a neutral pH was more efficient than that at alkaline pH. The determination of total protein showed that the protein content was similar among cultivars, ranging from 87.9 to 92.7 mg/g dry weight. Additionally, the water/NaCl-soluble protein fraction, containing 14${\sim}$16 kDa albumin and 22 kDa globulin ${\alpha}$-globulin, was also similar among cultivars, with a range of 9.94 to 11.98 mg/g dry weight. The SDS-PAGE/densitometry of total protein showed that there was no discernable difference in proteins of higher molecular weights among various cultivars, whereas the amount of lower molecular weight proteins (14${\sim}$16 kDa) is somewhat variable among cultivars. Furthermore, SDS-PAGE analysis of water/NaCl-soluble and propanol-soluble fractions indicates that there is a discernible change in the content of albumin, globulin or prolamin among cultivars. Thus, the PAGE/densitometry method, preceded by solubility fractionation, is useful for examining differences in protein profiles of rice cultivars.
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Protein Profiles of Major Korean Rice Cultivars
김미리,Chuanha Wei,Oh Yun Kwon,Xi-Wen Liu,Hyoung Chin Kim,윤원기,Hwan Mook Kim 한국식품영양과학회 2007 Preventive Nutrition and Food Science Vol.12 No.2
The protein profiles among Korean rice cultivars were assessed by total protein determination, solubility frac- tionation, SDS-PAGE analysis and scanning densitometry. In the extraction of protein, the SDS/urea system at a neutral pH was more efficient than that at alkaline pH. The determination of total protein showed that the protein content was similar among cultivars, ranging from 87.9 to 92.7 mg/g dry weight. Additionally, the water/NaCl-soluble protein fraction, containing 14~16 kDa albumin and 22 kDa globulin α-globulin, was also similar among cultivars, with a range of 9.94 to 11.98 mg/g dry weight. The SDS-PAGE/densitometry of total protein showed that there was no discernable difference in proteins of higher molecular weights among various cultivars, whereas the amount of lower molecular weight proteins (14~16 kDa) is somewhat variable among cultivars. Furthermore, SDS-PAGE analysis of water/NaCl-soluble and propanol-soluble fractions indicates that there is a discernible change in the content of albumin, globulin or prolamin among cultivars. Thus, the PAGE/densitometry method, preceded by solubility fractionation, is useful for examining differences in protein profiles of rice cultivars.
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