Growth Characteristics of Dendropanax morbifera in Chonnam

Choi,Seong-Kyu,Yun,Kyeong-Won,Lee,Jong-Il 한국자원식물학회 2002 Plant Resources Vol.5 No.2

This study was carried out obtain basic information for growth characteristics by different age of Dendropanax morbifera in chonnam, such as Yosu, Sunchon, Haenam, Gangjin, Wando. The pH of soil in cultivation area was 5.2 to 5.6 and organic matter was very high up to 10.6% . P205 content of soil in cultivation area was 35.3 to 42.1 mg/L, C.E.S was 13.9 to 14.4 me/100g, and moisture rate was 19.2 to 21.1 % . The flowering of Dendropanax morbifera began from 6~7 years old tree. The flowering date was at 10th of July at Wando. The growth characteristics of 12 years old tree was 929.5cm in stem height, 134.1mm in stem diameter, and 15 years old tree was 1,117.9cm in stem height, 160.8mm in stem diameter. The number of leaf was 13.9 at five years old tree, and the petioles length was 12.6 at five years old tree. Xylem sap can be had at more than 10 years old tree with good growth more than 10 em stem diameter.

Effect of Storage Condition on Yield and Quality of Angelica acutiloba Radix

Choi,Seong-kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-nam 한국자원식물학회 2002 Plant Resources Vol.5 No.1

To develop an optimal storage method of root of Angelica acutiloba Radix, which has been grown as major herbal medicine material to be cultivated, root of Angelica acutiloba Radix, was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. In conclusion, vacuum packing and complete sealing with polyethylene film appears to be most optimal for storage of Angelica acutiloba Radix.

A Basic Study on Leaf and Stem Production of Angelica acutiloba

Choi,Seong-Kyu,Yun,Kyeong-won,Chon,Sang-Uk,Lee,Jong-Ill,Seo,Young-Nam,Seo,Kyoung-Sun,Choi,Kyeong-Ju 한국자원식물학회 2004 Plant Resources Vol.7 No.3

This study was conducted to develop effective production system in greenhouse for leaves and stems of Angelica acutiloba by using connect pots in 2003 and 2004. Seed germination rate and plant biomass of Angelica acutiloba collected in 2004 were higher than those harvested in 2003. Germination rate of Angelica acutiloba seeds collected in 2003 was 10%, while germination rate of seeds collected in 2004 was above 90%. Especially, plant growth and yield of Angelica acutiloba grown in connect pots sized with 4×4×5cm(length×width×height) were the highest. These results indicate that leaf and stem production of Angelica acutiloba can be improved by using connect pots and optimizing seed collecting time in greenhouse.

A Study on Storage of Major Herbal Medicine Materials, Zingiberis Rhizoma

Choi,Seong-Kyu,Yun,Kyeong-Won,Shin,Kil-Man 한국자원식물학회 2002 Plant Resources Vol.5 No.3

To develop optimal storage method of root of Zingiberis Rhizoma, which has been grown as major cultural herbal medicine materials, root of Zingiberis Rhizoma, was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. Conclusionally, vacuum packing and complete sealing with polyethylene film appears to be optimal for storage of Zingiberis Rhizoma.

A Study on Storage of a Major Herbal Medicine Material, Peucedani Radix

Choi,Seong-Kyu,Yun,Kyeong-Won,Kwon,Byung-Sun 한국자원식물학회 2002 Plant Resources Vol.5 No.2

To develop optimal storage method of root of Peucedani Radix, which has been grown as major cultural herbal medicine material, root of Peucedani Radix was stored for 10 months with different packing materials and sealing methods. The loss in dry weight as influenced by packing materials and sealing methods was the lowest at vacuum packing and followed by complete sealing methods with transparent polyethylene film. The ratio of root rot during the storage period was not significantly different between packing materials but was significantly different between sealing conditions. Conclusively vacuum packing and complete sealing with polyethylene film appears to be optimal for storage of Peucedani Radix.

Temperature Effect on Seed Germination and Seedling Growth of Angelica acutilobu

Choi,Seong-Kyu,Yun,Kyeong-Won 한국자원식물학회 2002 Plant Resources Vol.5 No.3

Special objective of this study is to investigate morphological characteristics of seeds and effects of environmental factors such as light and temperature on their germination and seedling growth of Angelica acutilobu in Umbelliferae plants, using them as oriental medicine materials. Seed shape of Angelica acutilobu is ovate. Color of seed coats varied from yellow to dark brown. Seed size was 5.5 mm in length, 4.0mm in width. One thousand-seed weight of Angelica acutilobus was 3,210mg. Optimum temperatures for seed germination and seedling growth ranged from 20 to 25℃

Study on Leaf Production of Angelica acutiloba by Mulching with Polyethylene Film

Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-sun,Kwon,Byung-Sun 한국자원식물학회 2003 Plant Resources Vol.6 No.3

This study was conducted to produce effectively more leaves of Angelica acutiloba through year-round production system by using various mulching materials and polyethylene(PE) tunnel. Soil temperature by covering treatment was higher in PE mulching and PE tunnel than in rice straw mulching, especially showing the highest temperature in PE tunnel from April through May. Water content of soil was higher in PE mulching than in non-mulching. Leaf production of Angelica acutiloba through year-round production showed the highest in PE tunnel and root production in PE mulching. These results suggest that leaf production of Angelica acutiloba can be maximized through PE tunnel cultivation system.

Effect of Activated Charcoal on Growth of Zingiber mioga ROSC

Choi,Seong-Kyu,Yun,Kyeong-Won,Chon,Sang-Uk,Seo,Young-Nam,Seo,Kyoung-Sun,Kwon,Byung-Sun 한국자원식물학회 2004 Plant Resources Vol.7 No.2

Activate charcoal (AC) can be utilized as a soil conditioner in agricultural crop areas. This study was conducted to investigate the effect of AC on growth and yield of Zingiber mioga ROSC as affected by different amounts of AC. The results obtained are summarized as follows. Growth characteristics including plant height and leaf length were the highest when activated charcoal added with 10-30%, suggesting that optimum amount of activated charcoal was ranged from 10 to 30%. Growth and enlargement of the root were improved by 10% AC with higher rhizome length and weight.

Effect of Activated Charcoal on Growth of Curcuma longa Linne

Choi,Seong-Kyu,Yum,Kyeong-Won,Chon,Sang-Uk 한국자원식물학회 2003 Plant Resources Vol.6 No.3

This study was conducted to investigate the effect of Activated charcoal (AC) for stimulating growth and productivity of Curcuma longa Linne as affected by different concentrations of AC. Growth characteristics such as plant height and leaf length were the highest with the optimum amount of activated charcoal ranged from 10 to 30%. Root growth was elongated by the addition of 30% AC with the highest rhizome length and weight.

Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.