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RISS 인기검색어
- 검색키워드
- 저자 : Chatuphon Siamphan (검색결과 4 건)
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Siamphan, Chatuphon,Kim, Hyungjin,Lee, Jung-Sook,Kim, Wonyong International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.5
<P>A Gram-stain-negative, non-spore-forming, motile, strictly aerobic bacterial strain, designated CAU 1294<SUP>T</SUP>, was isolated from a sand sample and its taxonomic position was investigated using a polyphasic approach. The strain grew optimally at pH 6.5 and 30 °C and in the presence of 2 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1294<SUP>T</SUP> formed a lineage with member of the genus <I>Sneathiella</I> and exhibited similarity to <I>Sneathiella glossodoripedis</I> MKT133<SUP>T</SUP> (96.3 % similarity) and <I>Sneathiella chinensis</I> LMG 23452<SUP>T</SUP> (95.1 % similarity). Strain CAU 1294<SUP>T</SUP> contained Q-10 as the predominant respiratory quinone. C<SUB>18 : 1</SUB>ω7<I>c</I>, C<SUB>16 : 0</SUB> and cyclo-C<SUB>19 : 0</SUB>ω8<I>c</I> were the major cellular fatty acids. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine and two unidentified phospholipids. The DNA G+C content was 56.6 mol%. On the basis of these results, strain CAU 1294<SUP>T</SUP> is considered to represent a novel species of the genus <I>Sneathiella</I>, for which the name <I>Sneathiella chungangensis</I> is proposed. The type strain is CAU 1294<SUP>T</SUP> ( = KCTC 32476<SUP>T</SUP> = CECT 8513<SUP>T</SUP>). An emended description of the genus <I>Sneathiella</I> is also proposed.</P>
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Siamphan, Chatuphon,Kim, Wonyong International Union of Microbiological Societies 2014 International journal of systematic and evolutiona Vol.64 No.5
<P>A Gram-stain-negative, non-motile, rod-shaped, strictly aerobic bacterial strain, designated CAU 1289<SUP>T</SUP>, was isolated from a marine sand and its taxonomic position was investigated using a polyphasic approach. It grew optimally at pH 6.5 and 30 °C and in the presence of 3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1289<SUP>T</SUP> belonged to the genus <I>Salegentibacter</I>, exhibiting sequence similarity values of 93.6–96.3 % to members of this genus, and was related most closely to <I>Salegentibacter mishustinae</I> KMM 6049<SUP>T</SUP> (96.3 % similarity). Strain CAU 1289<SUP>T</SUP> contained MK-6 as the predominant menaquinone. The major fatty acid was iso-C<SUB>15 : 0</SUB>. The cell-wall peptidoglycan of strain CAU 1289<SUP>T</SUP> contained <I>meso</I>-diaminopimelic acid. The polar lipids were composed of phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine, an unidentified phospholipid, an unidentified aminolipid, an unidentified aminophospholipid, an unidentified glycolipid and ten unidentified lipids. The DNA G+C content was 38.7 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic inference, strain CAU 1289<SUP>T</SUP> is considered to represent a novel species of the genus <I>Salegentibacter</I>, for which the name <I>Salegentibacter chungangensis</I> sp. nov. is proposed. The type strain is CAU 1289<SUP>T</SUP> (KCTC 24000<SUP>T</SUP> = CCUG 64793<SUP>T</SUP>). An emended description of the genus <I>Salegentibacter</I> is also proposed.</P>
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Eudoraea chungangensis sp. nov., isolated from an aquafarm waste water sludge.
Siamphan, Chatuphon,Chang, Young-Hyo,Kim, Wonyong N.V. Swets en Zeitlinger 2015 Antonie van Leeuwenhoek Vol.107 No.4
<P>A Gram-stain negative, non-spore-forming, non-motile, strictly aerobic bacterial strain, designated CAU 1296(T), was isolated from an aquafarm waste water sludge and its taxonomic position was investigated using a polyphasic approach. Cells of strain CAU 1296(T) grew optimally at 30?C, at pH 7.5 and in the presence of 4?% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CAU 1296(T) formed a distinct lineage within the genus Eudoraea and exhibited similarity to Eudoraea adriatica AS06/20a(T) (95.4?% similarity). The major cellular fatty acids of the isolate were iso-C15:0, iso-C15:1 G, and summed feature 3 (C16:1 ω6c and C16:1 ω7c). The polar lipid pattern of strain CAU 1296(T) consisted of phosphatidylethanolamine, and unidentified lipids including a phosphoglycolipid, phospholipid, glycolipid, aminophospholipid, two aminolipids, and six further unidentified lipids. The strain contained menaquinone-6 (MK-6) as the major isoprenoid quinone. The G+C content of the genomic DNA was 38.7?mol%. On the basis of phenotypic, chemotaxonomic, and phylogenetic data, strain CAU 1296(T) should be classified as a novel species in the genus Eudoraea, for which the name Eudoraea chungangensis sp. nov. is proposed. The type strain is CAU 1296(T) (=KCTC 42048(T), =CCUG 66239(T), =CECT 8744(T)).</P>
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Jantima Arnthong,Chatuphon Siamphan,Charuwan Chuaseeharonnachai,Nattawut Boonyuen,Surisa Suwannarangsee 한국미생물·생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.11
The substantial use of fungal enzymes to degrade lignocellulosic plant biomass has widely been attributed to the extensive requirement of powerful enzyme-producing fungal strains. In this study, a two-step screening procedure for finding cellulolytic fungi, involving a miniaturized culture method with shake-flask fermentation, was proposed and demonstrated. We isolated 297 fungal strains from several cellulose-containing samples found in two different locations in Thailand. By using this screening strategy, we then selected 9 fungal strains based on their potential for cellulase production. Through sequence-based identification of these fungal isolates, 4 species in 4 genera were identified: Aspergillus terreus (3 strains: AG466, AG438 and AG499), Penicillium oxalicum (4 strains: AG452, AG496, AG498 and AG559), Talaromyces siamensis (1 strain: AG548) and Trichoderma afroharzianum (1 strain: AG500). After examining their lignocellulose degradation capacity, our data showed that P. oxalicum AG452 exhibited the highest glucose yield after saccharification of pretreated sugarcane trash, cassava pulp and coffee silverskin. In addition, Ta. siamensis AG548 produced the highest glucose yield after hydrolysis of pretreated sugarcane bagasse. Our study demonstrated that the proposed two-step screening strategy can be further applied for discovering potential cellulolytic fungi isolated from various environmental samples. Meanwhile, the fungal strains isolated in this study will prove useful in the bioconversion of agricultural lignocellulosic residues into valuable biotechnological products.
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