패턴 시를 활용한 초등영어 읽기 및 쓰기 지도

채찬희(Chae, Chanhee),이선(Lee, Sun) 한국영어교과교육학회 2010 영어교과교육 Vol.9 No.3

The purpose of this study is to examine the effects of teaching English reading and writing in elementary school through pattern poetry activities. Two classes of 5th graders participated in this study. Twenty nine students in experimental group wrote pattern poetry in the third period of each lesson individually or in groups. Thirty students in the control group did regular writing activities provided in the elementary English textbook. For the analysis of the study, pre and post tests on students’ English reading and writing and questionnaires were administerd and compared. Also students in the experimental group wrote comments after writing a pattern poem and these written comments were qualitatively analyzed. The qualitative and quantitative analysis of the study indicated that: first, writing pattern poem activity succeeded in enhancing the student’s interest, boosting their self-confidence and motivating students to participate in English class. Second, this activity had positive effects on the intermediate and low level students’ reading and writing ability. During the experiment, some students were observed to try to write pattern poems with their own creative ideas. And they enjoyed sharing their works with their classmates. Participants also showed some positive changes in their reading and writing ability and also their attitude towards English learning. Based on the results of the study, pedagogical implications are suggested.

Optimal probe size and fixation time for the detection of Porcine circovirus-2 DNA by in situ hybridization in formalin-fixed, paraffin-embedded tissue.

Ha, Yooncheol,Chae, Chanhee AAVLD 2009 JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION Vol.21 No.5

<P>Probe size and fixation time for detecting Porcine circovirus-2 (PCV-2) by in situ hybridization in formalin-fixed, paraffin-embedded lymph nodes from experimentally infected pigs were optimized. In situ hybridization using a 169-base pair (bp) probe detected significantly fewer PCV-2-positive cells than when using 8 other larger probes (P < 0.05). The difference in hybridization intensity between smaller probes (169 and 225 bp) and larger probes (416, 473, 571, 631, 693, and 753 bp) was statistically significant (P < 0.05). The PCV-2-positive cells were consistently detected in lymph nodes fixed up to 3 days; thereafter, the number of positive cells declined. The PCV-2-positive cells were detected in lymph nodes fixed for up to 730 days. The difference in hybridization intensity between samples fixed for a short term (1 or 3 days) and a longer term (4-730 days) was statistically significant (P < 0.05). The data demonstrates that the optimal probe size and fixation time for detecting PCV-2 in formalin-fixed, paraffin-embedded lymph nodes is 473 bp and 1-3 days, respectively.</P>

Construction and Characterization of an <i>Actinobacillus pleuropneumoniae</i> Serotype 2 Mutant Lacking the Apx Toxin Secretion Protein Genes <i>apxIIIB</i> and <i>apxIIID</i>

PARK, Changbo,HA, Yooncheol,KIM, Soohee,CHAE, Chanhee,RYU, Doug-Young Japanese Society of Veterinary Science 2009 The Journal of veterinary medical science Vol.71 No.10

<P>Apx toxins have been identified as important virulence factors of <I>Actinobacillus pleuropneumoniae</I>, the etiologic agent of porcine pleuropneumonia. In some <I>A. pleuropneumoniae</I> serotypes, Apx toxins are secreted by the cell membrane proteins encoded by <I>apxIIIB</I> and <I>apxIIID</I> genes. In an effort to develop a live vaccine strain against <I>A. pleuropneumoniae</I>, we inactivated the <I>apxIIIB</I> and <I>apxIIID</I> genes in <I>A. pleuropneumoniae</I> 1536, a serotype 2 strain, resulting in the Δ<I>apxIIIB/DapxIIID</I> mutant strain (1536ΔBΔD). Immunization of pigs with live 1536ΔBΔD <I>A. pleuropneumoniae</I> conferred protection against homologous challenge with wild-type <I>A. pleuropneumoniae</I> 1536. Thus, impaired Apx toxin secretion may decrease the virulence of <I>A. pleuropneumoniae</I> and may be an effective strategy for the development of a live-attenuated <I>A. pleuropneumoniae</I> vaccine.</P>

Vaccination of sows against type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) before artificial insemination protects against type 2 PRRSV challenge but does not protect against type 1 PRRSV challenge in late gestation

Han, Kiwon,Seo, Hwi Won,Park, Changhoon,Chae, Chanhee BioMed Central 2014 VETERINARY RESEARCH Vol.45 No.-

<P>The objective of the present study was to determine the effects of the commercially available type 2 Porcine Reproductive and Respiratory Syndrome Virus (PRRSV)-based modified live vaccine against type 1 and type 2 PRRSV challenge in pregnant sows. Half of the sows in the study were vaccinated with a type 2 PRRSV-based vaccine 4 weeks prior to artificial insemination while the other half remained non-vaccinated. Sows were then challenged intranasally with type 1 or type 2 PRRSV at 93 days of gestation. The sows which received the type 2 PRRSV-based vaccine followed by type 2 PRRSV challenge had significantly higher neutralizing antibody titers against type 2 PRRSV than they did against type 1 PRRSV. These same sows had higher frequencies of IFN-γ-secreting cells when stimulated with type 2 PRRSV compared to those stimulated with type 1 PRRSV. Subsequent virological evaluation demonstrated that the type 2 PRRSV-based vaccine reduced the type 2 PRRSV load but not the type 1 PRRSV load present in the blood of the sows. Additionally, vaccination of pregnant sows with the type 2 PRRSV-based vaccine effectively reduced the level of type 2 PRRSV nucleic acids observed in fetal tissues from type 2 PRRSV-challenged sows but did not reduce the level of type 1 PRRSV nucleic acid observed in fetal tissues from type 1 PRRSV-challenged sows. This study demonstrates that the vaccination of pregnant sows with the type 2 PRRSV-based vaccine protects against type 2 PRRSV challenge but does not protect against type 1 PRRSV challenge.</P>

Comparative Study of <i>In situ</i> Hybridization and Immunohistochemistry for the Detection of Porcine Circovirus 2 in Formalin-Fixed, Paraffin-Embedded Tissues

KIM, Duyeol,HA, Yooncheol,LEE, Yong-Hoon,CHAE, Sungwon,LEE, Kichan,HAN, Kiwon,KIM, Junghyun,LEE, Ju-Ho,KIM, Sung-Hoon,HWANG, Kyu-Kye,CHAE, Chanhee Japanese Society of Veterinary Science 2009 The Journal of veterinary medical science Vol.71 No.7

<P><I>In situ</I> hybridization and immunohistochemistry with different types of antibody (monoclonal vs. polyclonal, natural vs. synthetic) was compared to detect porcine circovirus 2 (PCV2) in formalin-fixed, paraffin-embedded tissues from pigs with experimentally and naturally occurring postweaning multisystemic wasting syndrome. PCV2 DNA and antigen was detected in tissues from both experimentally and naturally infected pigs by <I>in situ</I> hybridization and immunohistochemistry, respectively. Statistical evaluation revealed that more PCV2 positive signals were significantly detected in both experimentally and naturally infected pigs by <I>in situ</I> hybridization compared with immunohistochemistry (<I>P</I><0.05). The results of this study demonstrated that<I> in situ</I> hybridization proved more sensitive than immunohistochemistry for the detection of PCV2 in formalin-fixed, paraffin-embedded lymph node tissues.</P>

Effect of porcine circovirus type 2 (PCV2) vaccination on PCV2-viremic piglets after experimental PCV2 challenge

Seo, Hwi Won,Park, Changhoon,Han, Kiwon,Chae, Chanhee BioMed Central 2014 Veterinary research Vol.45 No.-

<P>The objective of this study was to evaluate the effect of porcine circovirus type 2 (PCV2) vaccines on PCV2-viremic and -seropositive piglets born from naturally PCV2-infected sows against postnatal PCV2 challenge. The experimental design was aimed at mimicking commercial swine rearing conditions to evaluate the response of the PCV2 vaccine on PCV2-viremic and -seropositive piglets after experimental PCV2 challenge. PCV2a (or 2b)-viremic piglets received a PCV2 vaccine at 21 days of age followed by a PCV2b (or 2a) challenge at 49 days of age (28 days post vaccination). The PCV2 vaccines elicited a high level of humoral (as measured by immunoperoxidase monolayer assay and neutralizing antibody titers) and cellular (as measured by the frequency of PCV2-specific interferon-γ-secreting cells) immune response in the PCV2-viremic piglets after vaccination even in the presence of maternally derived antibodies (MDA). The initial infection of PCV2 in the pigs was not affected by PCV2 vaccination, however the challenging PCV2 was reduced by PCV2 vaccination on PCV2-viremic pigs. The results from this study demonstrate that the PCV2 vaccine used in this study is effective at reducing PCV2 viremia and lymphoid PCV2 DNA, even for PCV2-viremic pigs with passively acquired MDA at the time of vaccination.</P>

Comparison of Four Commercial One-Dose Porcine Circovirus Type 2 (PCV2) Vaccines Administered to Pigs Challenged with PCV2 and Porcine Reproductive and Respiratory Syndrome Virus at 17 Weeks Postvaccination To Control Porcine Respiratory Disease Complex

Park, Changhoon,Seo, Hwi Won,Han, Kiwon,Chae, Chanhee American Society for Microbiology 2014 CLINICAL AND VACCINE IMMUNOLOGY Vol.21 No.3

<P>Under Korean field conditions, coinfection with porcine circovirus type 2 (PCV2) and porcine reproductive and respiratory syndrome virus (PRRSV) is most commonly observed in porcine respiratory disease complex (PRDC). Despite the wide use of PCV2 vaccination, PRDC remains a serious respiratory problem. Thus, the objective of this study was to determine and compare the efficacy of 4 one-dose PCV2 vaccines on 3-week-old pigs with an experimental PCV2-PRRSV challenge at 17 weeks postvaccination. Regardless of which commercial PCV2 vaccine was used, the vaccination of piglets at 3 weeks of age was efficacious against cochallenge of PCV2 and PRRSV, on the basis of growth performance and PCV2-associated lesions. However, the inactivated chimeric PCV1-2 and the PCV2 vaccines induced higher PCV2-specific neutralizing antibody (NA) titers and PCV2-specific gamma interferon-secreting cells and lower PCV2 viremia levels than the two PCV2 subunit vaccines. The vaccination of piglets against PCV2 at 3 weeks of age was effective in reducing PCV2 viremia and PCV2-associated lesions during the finishing period, which is an age at which pigs are frequently affected by PRDC caused by coinfection with PCV2 and PRRSV under Korean field conditions.</P>

Roles of p38 and JNK mitogen-activated protein kinase pathways during cantharidin-induced apoptosis in U937 cells

Huh, Jeong-Eun,Kang, Kyung-Sun,Chae, Chanhee,Kim, Hyung-Min,Ahn, Kyoo-Seok,Kim, Sung-Hoon WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2004 東西醫學硏究所 論文集 Vol.2004 No.-

Cantharidin is an active compound from blister beetles traditionally used for the treatment of cancer. It is known to exert its antitumor activity by inducing apoptosis in cancer cells. However, its signaling pathway still remains unclear. Therefore, we investigated the roles of the mitogen-activated protein kinases (MAPKs) and the tumor suppressor gene, p53, during cantharidin-induced apoptosis in U937 human leukemic cells. Cantharidin effectively activated ERK-1/2, p38 and JNK in U937 cells in a time- and dose-dependent manner. Cantharidin also exhibited a strong cytotoxicity and induced apoptosis in U937 cells. For the evaluation of the role of MAPKs, PD98059, SB202190 and SP600125 were used as MAPK inhibitors for ERK-1/2, p38 and JNK. PD98059 did not affect cantharidin-induced cytotoxicity and apoptosis, whereas SB202190 and SP600125 significantly interfered with cytotoxic and apoptotic activities induced by cantharidin. Cantharidin alone induced the apoptosis by phosphorylation of p53, up-regulation of downstream target genes, MDM2 and p21 and also cleaved caspase-3, whereas SB202190 and SP600125 caused the down-regulation of p53, MDM-2, p21 and cleaved caspase-3 after a co-treatment with cantharidin. Similarly, SB202190 and SP600125 significantly disturbed the caspase-3 activity after a co-treatment with cantharidin by colorimetric assay. Taken together, these results suggest that cantharidin can induce apoptosis by activation of p38 and JNK MAP kinase pathways associated with p53 and caspase-3.

Immunohistochemical identification of porcine reproductive and respiratory syndrome virus antigen in the lungs of naturally infected piglets

천두성,민경섭,채찬희,Cheon, Doo-Sung,Min, Kyoungsub,Chae, Chanhee The Korean Society of Veterinary Science 1997 大韓獸醫學會誌 Vol.37 No.2

돼지 생식기 호흡기 증후군 바이러스의 nucleocapsid와 반응을 하는 SDOW17 단크론항체를 이용하여 중성 포르말린에 고정시킨 자연감염된 포유자돈의 폐장에서 면역조직화학법을 이용하여 돼지 생식기 호흡기 증후군 바이러스 항원을 확인하였다. 서울대학교 수의과대학 병리학교실에 의뢰된 포유자돈들 중에서 병리조직학적으로 폐장에서 간질성 폐렴이 관찰된 포유자돈 7두를 임의로 선택하여 본 실험을 실시하였다. 간질성 폐렴의 병변으로 많은 수의 대식세포 침윤을 동반한 폐포벽 두께의 증가와 제II형 폐포세포의 비후가 관찰되었다. 검사한 7두 포유자돈중에서 6두에서 돼지 생식기 호흡기 증후군 바이러스에 대한 항체를 enzyme-linked immunosorbent assay에 의해 확인하였다. SDOW17 단크론항체를 이용한 면역조직화학염색과 간질성 폐렴의 대식세포에서 돼지 생식기 호흡기 증후군 바이러스의 항원을 검출하였고, 항원은 (주로)대식세포의 세포질에서만 진한 갈색의 양성반응이 관찰되었다. 이상 검사결과 돼지 생식기 호흡기 증후군 바이러스는 폐장의 간질과 폐포강에 분포되어 있는 대식세포에서 주로 증식하는 것으로 판명되었다. 본 실험에서 사용한 면역조직화학법은 돼지 생식기 호흡기 증후군 바이러스 감염여부를 바이러스 분리 또는 혈청검사 없이 진단하는데 사용할 수 있는 유용한 진단방법으로 판명되었다.

A New Modified Live Porcine Reproductive and Respiratory Syndrome Vaccine Improves Growth Performance in Pigs under Field Conditions

Park, Changhoon,Seo, Hwi Won,Kang, Ikjae,Jeong, Jiwoon,Choi, Kyuhyung,Chae, Chanhee American Society for Microbiology 2014 CLINICAL AND VACCINE IMMUNOLOGY Vol.21 No.9

<P>The change in growth performance resulting from a new modified live porcine reproductive and respiratory syndrome (PRRS) vaccine was evaluated under field conditions for registration with the government as guided by the Republic of Korea's Animal and Plant Quarantine Agency. Three farms were selected based on their history of PRRS-associated respiratory diseases. On each farm, a total of 45 3-week-old pigs were randomly allocated to one of two treatment groups, (i) vaccinated (<I>n</I> = 25) or (ii) control (<I>n</I> = 20) animals. A new modified live PRRSV vaccine increased market weight by 1.26 kg/pig (104.71 kg versus 103.45 kg; <I>P</I> < 0.05) and decreased mortality by 17% (1.33% versus 18.33%; <I>P</I> < 0.05). Pathological examination indicated that vaccination effectively reduced microscopic lung lesions compared with control animals on the 3 farms. Thus, the new modified live PRRS vaccine improved growth performance and decreased mortality and lung lesions when evaluated under field conditions.</P>