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- 저자 : C.m. Singh (검색결과 77 건)
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Singh, N.K.,Chae, H.S.,Hwang, I.H.,Yoo, Y.M.,Ahn, C.N.,Lee, H.J.,Park, H.J.,Chung, H.Y. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.3
Thiazolidinediones (TZDs) act as potent activators of the adipose differentiation program in established preadipose cell lines. TZD's have also been investigated in diabetic patients and reported to act as PPAR-${\gamma}$ ligands. In this report, the effects of TZDs on the differentiation pathway of myoblasts have been investigated. C2C12 mouse myoblasts were grown in Dulbecco's Modified Eagles medium for 4-5 days until they reached almost 100% confluency. Post-confluent cells (day 0) were further exposed to adipogenic induction medium along with TZDs for 48 hours. Thereafter, cells were exposed only to TZDs every 48 h until day 10. The control was provided with differentiation medium without any treatment. Alterations in the cells during the differentiation programme were analyzed on the basis of fusion index, oil-red-o staining, adipocyte index, adipocyte stain uptake measurement, immuno-histochemistry and western blotting. Exposure of C2C12 mouse myoblasts to TZDs prevented the expression of myosin heavy chain with parallel increase in the expression of C/EBP-${\alpha}$ and PPAR-${\gamma}$ and acquisition of adipocyte morphology, thus abolishing the formation of multinucleated myotubes. TZDs exert their adipogenic effects only in non-terminally differentiated myoblasts; myotubes were insensitive to the compound. Continuous exposure (at least 4-5 doses) to inducers after the growth arrest was essential to provide a sustained environment to the cells converting to fully matured adipoctyes. The results indicate that TZDs specifically converted the differentiation pathway of myoblasts into that of adipoblasts.
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Singh, N.K.,Chae, H.S.,Hwang, I.H.,Yoo, Y.M.,Ahn, C.N.,Lee, H.J.,Park, H.J.,Chung, H.Y. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.7
This study was conducted to determine the difference between satellite cells (porcine) and myoblasts (C2C12) in their differentiation under the influence of 2, 4-thiazolidindion. C2C12 myoblast cells and porcine satellite cells (isolated from 10 d old $Landrace{\times}Duroc$ piglets) were grown to absolute confluency. Post confluent cells (day 0) were further exposed to adipogenic induction medium along with 2, 4-thiazolidindion ($8{\mu}M$) for 2 d. Thereafter, cells were exposed to 2, 4-thiazolidindion alone every 2 d till day 10 and analysed. The control was cultured in differentiation medium without any treatment. Increased (p<0.05) expression of transcriptional factors i.e. C/EBP-${\alpha}$ and PPAR-${\gamma}$ and transition of cells to adipocyte morphology was noticed from 2 d and 4 d onwards in satellite cells (Porcine) and myoblasts (C2C12) respectively. Myogenesis was observed to be suppressed completely in case of satellite cells compared to myoblasts in response to 2, 4-thiazolidindion. Pax-7 (transcriptional factor) appeared as a sole entity to satellite cells only, as it was not identified in case of myoblasts. Although both the cells were converting to adipoblasts, the degree of their conversion was different in response to 2, 4-thiazolidindion. Therefore, the hypothesis that satellite cells contribute various domains to the growing myoblasts appeared obscured and found to be dependent on the proliferative energy/or degree of fusion. However, it revealed satellite cells as currency to myoblasts/muscle.
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Achromobacter panacis sp. nov., isolated from rhizosphere of Panax ginseng
Singh, P.,Kim, Y. J.,Singh, H.,Farh, M. E.,Yang, D. C. MICROBIOLOGICAL SOCIETY OF KOREA 2017 JOURNAL OF MICROBIOLOGY -SEOUL- Vol. No.
<P>A novel strain DCY105(T) was isolated from soil collected from the rhizosphere of ginseng (Panax ginseng), in Gochang, Republic of Korea. Strain DCY105(T) is Gram-reaction-negative, white, non-motile, non-flagellate, rod-shaped and aerobic. The bacteria grow optimally at 30 degrees C, pH 6.5-7.0 and in the absence of NaCl. Phylogenetically, strain DCY105(T) is most closely related to Achromobacter marplatensis LMG 26219(T) (96.81%). The DNA G+C content of strain DCY105(T) was 64.4 mol%. Ubiquinone 8 was the major respiratory quinone, and phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were amongst the major polar lipids. C-16:00, C(8:0)3OH and iso-C(17:0)3OH were identified as the major fatty acids present in DCY105(T). The results of physiological and biochemical tests allowed strain DCY105(T) to be differentiated phenotypically from other recognized species belonging to the genus Achromobacter. Therefore, it is suggested that the newly isolated organism represents a novel species, for which the name Achromobacter panacis sp. nov. is proposed with the type strain designated as DCY105(T) (=CCTCCAB 2015193(T) =KCTC 42751(T)).</P>
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Farh, M.E.A.,Kim, Y.J.,Sukweenadhi, J.,Singh, P.,Yang, D.C. G. Fischer 2017 Microbiological research Vol.200 No.-
<P>Panax ginseng is an important cash crop in the Asian countries due to its pharmaceutical effects, however the plant is exposed to various abiotic stresses, lead to reduction of its quality. One of them is the Aluminum (Al) accumulation. Plant growth promoting bacteria which able to tolerate heavy metals has been considered as a new trend for supporting the growth of many crops in heavy metal occupied areas. In this study, twelve bacteria strains were isolated from rhizosphere of diseased Korean ginseng roots located in Gochang province, Republic of Korea and tested for their ability to grow in Al-embedded broth media. Out of them, four strains (Pseudomonas simiae N3, Pseudomonas fragi N8, Chryseobacterium polytrichastri N10, and Burkholderia ginsengiterrae N11-2) were able to grow. The strains could also show other plant growth promoting activities e.g. auxins and siderophores production and phosphate solubilization. P. simiae N3, C. polytrichastri N10, and B. ginsengiterrae N11-2 strains were able to support the growth of Arabidopsis thaliana stressed by Al while P. fragi N8 could not. Plants inoculated with P. simiae N3, C. polytrichastri N10, and B. ginsengiterrae N11-2 showed higher expression level of Al-stress related genes, AtAIP, AtALS3 and AtALMT1, compared to non-bacterized plants. Expression profiles of the genes reveal the induction of external mechanism of Al resistance by P. simiae N3 and B. ginsengiterrae N11-2 and internal mechanism by C. polytrichastri N10. Korean ginseng seedlings treated with these strains showed higher biomass, particularly the foliar part, higher chlorophyll content than non-bacterized Al-stressed seedlings. According to the present results, these strains can be used in the future for the cultivation of ginseng in Al persisted locations.</P>
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Synthesis, magnetic and Mossbauer spectroscopic studies of Cr doped lithium ferrite nanoparticles
Srivastava, M.,Layek, S.,Singh, J.,Das, A.K.,Verma, H.C.,Ojha, A.K.,Kim, N.H.,Lee, J.H. Elsevier Sequoia 2014 JOURNAL OF ALLOYS AND COMPOUNDS Vol.591 No.-
Lithium-based ferrites are promising and potential magnetic materials for microwave applications. They possess a spinel (AB<SUB>2</SUB>O<SUB>4</SUB>) type crystal structure, where the distributions of metal cations over the tetrahedral (A) and octahedral (B) voids play a crucial role for exhibiting different physical properties. Among various parameter of synthesis, pH is an important factor that influences the cation distribution over A and B voids, overall growth of the nanoparticles and different physical properties. In the present work single phase Cr substituted lithium ferrite nanoparticles have been synthesized by the sol-gel method at different pH. The phase identification and crystallite size have been probed by X-ray diffraction studies. The crystallite size changes by 44.2-48.8nm upon varying the pH from 3.5 to 11.5. In order to investigate the cations distribution at A/B sites, Mossbauer spectroscopic measurements were done. The values of magnetic hyperfine field obtained from the Mossbauer data for the A and B sites are ~49.5T and 51T, respectively. Moreover, it is observed that the area ratio of B site to A site increases with increasing the pH. This observation further suggests that the B site is more preferable for Fe<SUP>3+</SUP> cations at higher pH than the A site. The magnetic parameter such as saturation magnetization (M<SUB>s</SUB>), remanent magnetization (M<SUB>r</SUB>), coercive field (H<SUB>C</SUB>) and squareness (S) are determined by vibrating sample magnetometer (VSM) measurements, which show a consistent increase with increasing pH. The reason for the variation in magnetic properties has been explained on the basis of increased Fe<SUP>3+</SUP> cation occupancies at the B site and size effect, which is well supported by Mossbauer spectroscopic and XRD studies.
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Sin, B.C.,Singh, L.,Lee, K.E.,Kim, M.,Cho, M.,Yarger, J.L.,Woo, S.K.,Lee, H.i.,Lee, Y. Elsevier Sequoia 2015 Journal of Electroanalytical Chemistry Vol.756 No.-
A potential cathode material for lithium ion batteries has been developed using a boron polyanion substituted lithium iron manganese phosphate, LiFe<SUB>0.4</SUB>Mn<SUB>0.6</SUB>(PO<SUB>4</SUB>)<SUB>1-x</SUB>(BO<SUB>3</SUB>)<SUB>x</SUB> (x=0 to 0.03). Without any external carbon source, the material has been synthesized by solid-state reaction using ball-mill and was subsequently characterized by X-ray diffraction, scanning electron microscopy, electron energy loss spectroscopy, X-ray photoelectron spectroscopy, cyclic voltammetry, and galvanostatic charge-discharge measurement. The LiFe<SUB>0.4</SUB>Mn<SUB>0.6</SUB>(PO<SUB>4</SUB>)<SUB>1-x</SUB>(BO<SUB>3</SUB>)<SUB>x</SUB> samples show a single-phase crystalline nature with X-ray diffraction analysis, and enhanced discharge capacity at various C-rates as compared to that of pure LiFe<SUB>0.4</SUB>Mn<SUB>0.6</SUB>PO<SUB>4</SUB>. Among several LiFe<SUB>0.4</SUB>Mn<SUB>0.6</SUB>(PO<SUB>4</SUB>)<SUB>1-x</SUB>(BO<SUB>3</SUB>)<SUB>x</SUB> samples, LiFe<SUB>0.4</SUB>Mn<SUB>0.6</SUB>(PO<SUB>4</SUB>)<SUB>0.995</SUB>(BO<SUB>3</SUB>)<SUB>0.005</SUB> demonstrated the best cycleability, exhibiting an initial discharge capacity of 159.4mAhg<SUP>-1</SUP> at 0.1C and 113mAhg<SUP>-1</SUP> at 3C. LiFe<SUB>0.4</SUB>Mn<SUB>0.6</SUB>(PO<SUB>4</SUB>)<SUB>1-x</SUB>(BO<SUB>3</SUB>)<SUB>x</SUB> demonstrates enhanced electrochemical properties with excellent reversible cycling via boron polyanion substitution.
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Saini, K.K.,Sharma, S.D.,Chanderkant,Kar, M.,Singh, D.,Sharma, C.P. North-Holland 2007 Journal of non-crystalline solids Vol.353 No.24
Nanocrystalline thin films of titanium dioxide have been fabricated on glass and silica substrates from partially hydrolyzed precursor solution. These films were subjected to heat treatment for 1h at temperatures 100, 200, 300, 400, 500, 600, 700, 800 and 900<SUP>o</SUP>C and characterized by XRD, SEM, XPS and optical techniques. As deposited films are found to be amorphous and also contain hydroxyl and organic functional groups. Films heat treated above 100<SUP>o</SUP>C do not contain hydroxyl and organic functional groups. Microcrystalline behavior is observed in the films heat treated above 300<SUP>o</SUP>C. Crystallite size increases from ∼5 to 50nm as sintering temperature is increased from 300 to 700<SUP>o</SUP>C. Formation of anatase phase with c-axis length 7.03A is observed in the films annealed up to 700<SUP>o</SUP>C. These films peel off from the substrate beyond 700<SUP>o</SUP>C annealing temperature. Density as well as refractive index of the films increases with increase in annealing temperature up to 700<SUP>o</SUP>C. Refractive index is found to show Cauchys behavior. Transmission better than 70% is observed in the visible range. There is a strong absorption around 370nm, which is attributed to band gap absorption of the material.
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Singh, M.P.,Han, J.,Kang, S.C. Masson Pub. USA, Inc 2017 BIOMEDICINE AND PHARMACOTHERAPY Vol.88 No.-
<P>Quercetin, a well cognized bioactive flavone possessing great medicinal value, has limited usage. The rapid gastrointestinal digestion of quercetin is also a major obstacle for its clinical implementation due to low bioavailability and poor aqueous solubility. 30,5-dihydroxy-3,40,7-trimethoxyflavone (DTMF), a novel semi-synthetic derivative of quercetin, is known to modulate several biological activities. Therefore, in the present study we examined the cytotoxic mechanism of DTMF in concentration-dependent manner (25, 50, and 100 mu M; 24 h) against HCT-116 human colon carcinoma cells. The cytotoxic potential of DTMF was characterized based on deformed cell morphology, increased ROS accumulation, loss of mitochondrial membrane potential (Delta psi m), increased mitochondrial mass, chromatin condensation, and typical DNA-fragmentation in HCT-116 cells. The results showed that DTMF-induced enhanced ROS production at higher concentration (100 mu M) as evidenced by upregulated expression of ER stress and apoptotic proteins with concomitant increase in PERK, CHOP, and JNK levels, when compared to N-acetyl cysteine (NAC, ROS inhibitor) treated HCT-116 cells, which depicts that DTMF might act as a crucial mediator of apoptosis signaling. Collectively, our results suggest that DTMF stimulates ROS-mediated oxidative stress, which in turn induces PERK-CHOP and JNK pathway of apoptosis to promote HCT-116 cell death. (C) 2017 Elsevier Masson SAS. All rights reserved.</P>
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Singh, P.,Lee, H.C.,Silva, M.F.,Chin, K.B.,Kang, I. Elsevier 2017 FOOD CONTROL Vol.77 No.-
<P>The purpose of this research was to evaluate the effects of trisodium phosphate dip (TSP), hot water dip (HWD) and their combination dip with/without brushing on broiler breast skin for bacterial reduction and structural changes. Eviscerated broiler carcasses were obtained from a local slaughter plant and immediately subjected to one of six treatments: 1) two tap water dips at 25 degrees C (TWD/TWD), 2) TWD/ TWD with brushing (TWD/TWD/B), 3) TWD and TSP at 8%/25 degrees C (TWD/TSP), 4) TWD and HWD at 71 degrees C (TWD/HWD), 5) TSP and HWD (TSP/HWD), and 6) TSP/HWD with brushing (TSPIHWD/B). Each dip was conducted for 45 s with or without brushing at 5 s on/off during the second dip. Compared to the control (TWD/TWD), TSP/HWD significantly reduced mesophilic aerobic bacteria (MAB), Escherichia coli (E. coli), and total coliforms by 1.1, 0.9 and 1.0 log CFU/g, respectively, and Salmonella prevalence by 53.3% (P < 0.05), whereas TWD/TSP and TWD/HWD showed intermediate reductions (P < 0.05). Upon brushing, TSP/HWD/B reduced populations of MAB, E. coli, and total coliforms, and the prevalence of Salmonella more effectively than control of brushing (TWID/TWD/B) (P < 0.05). When two sampling methods were compared, the method of stomaching released fewer MAB and total coliforms (named loosely-associated cells) than the grinding of the stomached skin (named tightly-associated cells). Compared to controls (TWD/TWD and TWD/TWD/B), both TSP/HWD and TSP/HWD/B generally resulted in darker, less reddish, and more yellowish breast skin. Scanning electron microscope and histological images indicated that both TSP/HWD and TSP/HWD/B had deeper skin penetration than controls or TWD/HWD and TWD/TSP. Overall, TSP/HWD/B showed the most effectiveness in broiler carcass decontamination. (C) 2017 Published by Elsevier Ltd.</P>
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