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C. Harn(韓昶烈),M. S. Lee(李萬相),J. M. Kim(金鍾萬) 한국육종학회 1974 한국육종학회지 Vol.6 No.2
Pollen tube penetration and fertilization of Panax ginseng were investigated, the results of which we re summarized as follows: Pollen tube started to grow into the stigmatic tissue about five hours after pollination, reached to the embryo sac by about 16 hours after pollination. Fertilization of secondary nucleus occured about 18 hours after pollination, while the egg cell was fertilized seven hours later than the fusion nucleus. First division of the primary endosperm nucleus was observed taking place around 25 hours af ter pollination, but the fertilized egg cell underwent its first division about 20 hours later than the primary endosperm nucleus.
고추(2n=24) 染色體의 高次複合轉座 合成에 對한 試案
C. Harn(韓昶烈) 한국육종학회 1974 한국육종학회지 Vol.6 No.2
About 30 lines of ④ type reciprocal translocation were brained by irradiating the seeds of hot pepper (Capsicum annuum). Using these simple translocation lines, analysis of translocations and syntheses of multiple translocations are now under way. One cf the schematic pathways leading to the synthesis of ㉔ type multiple translocation from the ④ type Simple translocations is described in this paper.
C. Harn(韓昶烈),Y. Lee(李榮日) 한국육종학회 1975 한국육종학회지 Vol.7 No.1
Early proembryos of various stages ranging from 5 to 29 hours after flowering were X-rayed to examine seed formation of irradiated flowers, survival and fertility of M₁, plant, mutation rate, occurrence of chimaera and segregation ratio of albino. Developing proembryos seemed to be more sensitive to high dose than the free nuclei endosperm tissue. Fertility of M₁ plant were higher as compared with that of seed irradiation. Mutation rate based on albino and xantha was 5.6%, rather lower that those of seed irradiation, but higher when considered in terms of dose given. Plant based chimaeras were observed at every stages of proembryo development except the irradiation of fertilized egg. Segregation ratio of albino in mutated panicles of chimaeric plants was 0.06, while that of non-chimaeric plant was 0.20. Occurrence of chimaera in the irradiation at the early stage of proembryo is considered not true chimaera but the excessive recessive deficit.
벼 幼穗分化前後에 γ-線照射가 穗發達 및 葉緣體變異에 미치는 影響
C. Harn(韓昶烈),Y. I. Rhee(李榮日) 한국육종학회 1973 한국육종학회지 Vol.5 No.2
Present experiments were attempted to clarify the biological effects of gamma irradiation on the growing rice plants in terms of heading time, culm and panicle length, number and mean length of prima ry or secondary rachis branches per panicle, number of spikelets per panicle, and sterility in M₁, generation, and chlorophyll mutation in M₂ generation. 1. Radiosensitivity was highest when the rice plant was irradiated at stage immediaitely before the differentation of the panicle primordia. 2. Regardless of the growth stage at which the treatment was carried out, the panicle length was shortened and the number of both primary and secondary rachis branches were decreased. 3. Generally irradiation of growing plant brought about retardy heading date, and the delaying was conspicuous when irradiation was conducted at earlier stages of growth. 4. Frequency of chlorophyll mutation in M₂ generation was highest when the plants were irradiated 37 and 41 days before panicle emergence.
放射線處理한 벼 M₁이삭의 變異 sector에 關한 硏究
C. Harn(韓昶烈),J. L. Won(元鍾樂) 한국육종학회 1975 한국육종학회지 Vol.7 No.2
When mature seeds of cereal crops are treated with mutagenes, there occurs frequently the deficit of homo-recessive mutant in the M₁ generation, thus the segregation of recessiveness deviating from the expected ratio of 0.25. This has been considered caused partly by the function of the mutated gene and partly by the chimaeric nature of the M₁ panicle. Studies on the M₂ segregation inevitably led to the assumption of the size of mutated sector in the M₁ panicle and to the speculation on the number of initial cells (in the seed embryo) which would later form the sporogenous tissue of the M₁ panicle. So far the views concerning the initial cell have been conflicting and controvertial. X-rayed rice seeds were sown directly in the pots and all the tillers including the lower ones were induced to full growth to examine whether there exists any difference in the segregation ratio among the tillers. No definite tendencies or regularities in the segregation ratio among the tillers were observed. If the number of initial cells varies with the order of tiller emergence, i.e., main culm having several and the upper tillers having only one cell, there would be certain definite tendency in the segregation ratio among the tillers, with the upper tillers approaching the expected ratio, which was not proved in the present experiment. As it is assumed that the mutated cell, mutated gamete, and homo-recessive mutant might be affected by many factors, it does’nt seem to be correct to speculate the sector size in the M₁ panicle and the number of initial cells in the seed embryo simply by the M₂ sgregation ratio.
고추染色體의 複合轉座合成에 關한 硏究 1. 轉座 homo個體의 選拔
C. Harn(韓昶烈),J. L. Won(元鍾樂) 한국육종학회 1975 한국육종학회지 Vol.7 No.2
Thirty seven plants with ④ type simple reciprocal translocation of chromomoses was obtained by treating seed of hot pepper with X-ray. Selection of translocation homozygotes, numbering of chromosomes which have participated in the translocations, synthesis of multiple-translocations from simple ones were attempted for use in various genetical and breeding purposes. Present report, the first of series of articles which will be presented subsequently, describes mainly the procedural works on the induction of translocation and selection of translocation homozygotes.
手稻高蛋白 突然變異系統과 母品種과의 交雜에 있어서 多發性遺傳因子의 發現에 關한 硏究
C. HARN(韓昶烈),J. L. WON(元鍾樂) 한국육종학회 1976 한국육종학회지 Vol.8 No.2
High protein rice mutant (M₁₂ plant generation in 1976) was obtained from the progeny of X-rayed Hokwang variety. Besides high protein, the mutant accompanied two other mutated characters of reduced culm length and earlier heading date. Crossing between the mutant and its mother variety was made (1) to examine the gene(s) controlling high protein, (2) to verify the pleiotropic (or linkage) relation among the three changed characters and (3) to test the possibility of intro-ducing the high protein gene(s) into mother variety. The assumption that a single pleiotropic recessive gene might be responsible for the three mutated characters was evidenced in F₂ by the segregation of mother and mutant types in the ratio of 3 : 1. Besides these parental types there were numbers of segregant groups in F₂. Among them were types which were mother-like phenoty-pically but had as high protein content as the mutant. These segregants were placed under further experiment for the confirmation of the character manifestation or fixation in later generations.