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Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특성 분석(Ⅲ)
황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.16 No.2
To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.131 g/ml - 1.240 g/ml), and the other was the low density part (1.103 g/ml - 1.122 g/ml). There was not the high density part in the serum treated with PAS for removing of antibody bound HCV. Therefore, it was concluded that the HCV in high density portion were the antibody bound forms, and the HCV in the low density portion were the free forms.
황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.17 No.2
In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7)) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of Huh_(7) cells as the infectious sources. In the high density portion, the HCV genomes were not detected in the media and cells of all days except in the 2 day culture medium, but in the low density portion, the HCV genomes were detected in the media and cells of all days. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.
황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.17 No.2
In this research, It was investigated to increase the infectious efficacy of HCV to culture cells, and the efficacy of HCV cell culture system. To increase the infectious efficacy to human cells, The human hepatoma cell lines (Huh_(7),) was used as the culture cells to test the HCV infection. The whole patient serum, the high density portion, and the low density portion were added in the culture media of human hepatoma (Huh_(7)) cells as the infectious sources. It was showed that HCV partcles of the high density portion were not infectious to Huh_(7) cells, but HCV particles of the low density portion were infectious to Huh_(7) cells. Therefore, it was concluded that the antibody bound HCV was not infectious to Huh_(7) cells, but the antibody free HCV was infectious to Huh_(7) cells.
Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특징 분석(Ⅰ)
황태욱,유병제 대구대학교 기초과학연구소 2000 基礎科學硏究 Vol.16 No.2
To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.161 g/ml - 1.192 g/ml), and the other was the low density part (1.085 g/ml - 1.117 g/ml).
Sucrose density gradient에 의해 분리된 C형 간염 바이러스의 특성 분석(Ⅱ)
황태욱,유병제 大邱大學校附設 基礎科學硏究所 2000 基礎科學硏究 Vol.16 No.2
To study the buoyant density of HCV, the human patient sera was tested with both anti-HCV ELISA and RT-PCR for the detection of HCV. The patient sera, positive at two tests, were used for further experiments. After ultracentrifugation of 20-60% sucrose linear density gradient, the centrifugal medium containing HCV was fractionated by 280 ul from the bottom. The buoyant densities of all fractions were determined, and the HCV in all fractions were checked by the RT-PCR. There was the room to separate the fractions to two major parts containing HCV. One was the high density part (1.146 g/ml ∼ 1.207 g(ml), and the other was the low density part (1.089 glml ∼ 1.108 glml).
The Role of CDX2 in Intestinal Metaplasia Evaluated Using Immunohistochemistry
( Byoung Hwan Lee ),( Na Young Kim ),( Hye Seung Lee ),( Jung Mook Kang ),( Hyun Kyung Park ),( Hyun Jun Jo ),( Cheol Min Shin ),( Sang Hyub Lee ),( Young Soo Park ),( Jin Hyeok Hwang ),( Jin Wook Kim 대한소화기기능성질환·운동학회 2012 Gut and Liver Vol.6 No.1
Background/Aims: Intestinal metaplasia (IM) has been regarded as a premalignant condition. This study evaluated the role of the transforming factor CDX2 according to the severity and type of IM. Methods: This analysis was performed on 383 subjects with IM in the antrum and/or body, with diagnoses that were categorized as controls, dysplasias, and gastric cancers. The IM grades were classifi ed into four groups as negative, mild, moderate or severe using the updated Sydney scoring system. The IM subtypes were categorized as type I, type II, and type III using high iron diamine and alcian blue (pH 2.5) staining. The CDX2 expression in the IM foci was evaluated using immunohistochemistry in specimens from the antrum and/or body. Results: CDX2 expression increased according to IM severity (p=0.001) but was not associated with the IM subtype (p=0.881) in the antrum specimens. Similarly, CDX2 expression increased according to the IM grade (p=0.001) but was not associated with the IM subtype (p=0.755) in the body specimens. CDX2 expression was also increased according to baseline disease in the antrum, especially dysplastic and GC group (p=0.003), but not in the body (p=0.582). However, status of Helicobacter pylori infection was not associated with CDX2 expression in the antrum (p=0.692) and body (p=0.271). Conclusions: These results show that CDX2 expression is associated with the IM grade regardless of the IM subtype and that it was more frequent in the dysplasia group. These results suggest that CDX2 expression might play an important role in the progression of IM in various environments that can affect neoplastic change. (Gut Liver 2012;6:71-77)