http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Barbara Vizio (검색결과 2 건)
- 무료
- 기관 내 무료
- 유료
-
Graziella Bellone,Barbara Vizio,Tiziana Scirelli,Giorgio Emanuelli 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.3
Several clinical conditions may limit the success of bone regeneration and/or implant osseointegration. For this reason, many compounds have been tested for their ability to stimulate this biological process. Synthetic hydroxyapatite (HA), mimicking natural bone hydroxyapatite, and extra-cellular matrix proteins, such as type I collagen, are potential candidates. However, the synthetic origin of HA and the denaturing conditions required for extracting collagen from skin and derma are sources of potential drawbacks. This study examines the in vitro effects of a natural bone derivative (NBD) extracted from equine bone and containing both natural, non-synthetic bone hydroxyapatite and native, non-denatured, type I bone collagen as a possible active compound for stimulating bone regeneration and implant osseointegration. The activity of NBD was tested on bone marrow stromal cells (BMSCs), evaluating their growth/viability by the methylthiazol tetrazolium (MTT) assay and their migration potential by a scratch assay. Moreover, expression of the hyaluronic acid receptor (CD44) and the C-X-C chemokine receptor type 4 (CXCR4, CD184) on the surface of BMSCs was assessed by flow cytometry, and the release of Transforming Growth Factor (TGF)-b, Interleukin (IL)-1a and IL-6 was quantified using an enzyme-linked immunosorbent assay (ELISA). The effect of NBD-coated implants on human osteoblasts was tested by measuring alkaline phosphatase (ALP) activity with the p-nitrophenyl phosphate (pNPP) degradation test. NBD stimulated BMSC growth/viability, migration, CD184 surface expression and the release of TGF-b1. NBD-coated implants increased ALP activity of human osteoblasts. These results indicate that NBD may be an adjuvant to accelerate both bone regeneration and osseointegration.
-
Valentina Zunino,Giorgia Meineri,Graziella Bellone,Barbara Vizio,Adriana Prat,Maurizio Grandi,Elisabetta Radice,Federica Dal Bello,Claudio Medana 경희대학교 융합한의과학연구소 2017 Oriental Pharmacy and Experimental Medicine Vol.17 No.4
In recent years, there has been growing interest in plant-derived products for their bioactive properties., Results and Conclusion. This study aimed to verify the effect of an herbal-food supplement, comprising alcoholic extracts of Epilobium, Urtica dioica and Evernia prunastri (YATROS, Etnopharma, Turin, Italy), alone or in combination with 5-Fluorouracil, on cell growth, DNA synthesis, cell-cycle profile, cytoskeleton reorganization and E-cadherin protein expression, in the murine colon carcinoma cell line C26. Assays used were respectively the Neutral Red uptake assay, median effect analysis, tritiated thymidine incorporation, flow cytometry, Phalloidin staining, and immunohistochemistry. A chemical fingerprint and multidimensional HPLC-UV-HRMS analysis was also performed on the herbal-food supplements. Intriguingly, a substantial synergistic inhibitory effects on cell growth, DNA synthetic activity and percentage of cells in S phase were noticed when YATROS was combined with 5-Fluorouracil, reducing both dose and timing of exposure of C26 cells to the drug. Moreover, by inducing cytoskeleton reorganization and E-cadherin expression, YATROS can interfere with tumor cell invasiveness. These indications of an agonistic interaction between YATROS and 5-Fluorouracil in colon carcinoma cells may be of interest for clinical applications, and thus merit further investigation.
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
