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- 저자 : Bala Srinivasan (검색결과 2 건)
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Changing PEO coating formation on Mg alloys by particle additions to the treatment electrolyte
Carsten Blawert,Bala Srinivasan,Jun Liang,Yuanding Huang,Daniel Hoche,Nico Scharnagl,Volker Heitmann,Ulrich Burmester 한국표면공학회 2012 한국표면공학회 학술발표회 초록집 Vol.2012 No.11
Plasma electrolytic oxidation of magnesium alloys is a well known technique to produce corrosion and wear resistant coatings. The addition of particles to the electrolyte provides a possibility to produce coatings with an increasing range of composition by in-situ incorporation of those particles into the coating. An extensive literature review has revealed that the mode of incorporation depends mainly on the melting point of the used particles and the energy provided by the discharges of the PEO process. The spectrum ranges from inert to partly reactive incorporation, but a complete reactive incorporation and a formation of a new single phase coating was not observed so far. Thus a new approach in PEO processing is introduced using specific particles as a kind of sintering additive, changing not only the composition but lowering the melting temperature and increase the liquid phase fraction during the discharges, resulting in a new amorphous coating.
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Micropropagation and DNA Delivery Studies in Onion Cultivars of Bellary, CO3
Ashwini Malla,Balamurugan Srinivasan,Bala Murugan Shanmugaraj,Sathishkumar Ramalingam 한국작물학회 2015 Journal of crop science and biotechnology Vol.18 No.1
Onion is one of the most important vegetables grown all over the world. In the present study, rapidly growing and maintainable callus was accomplished using the basal meristem plate and twin scale leaves as explants in Bellary and CO3 varieties for callus induction and in vitro bulb formation. The two cultivars were transformed with Agrobacterium tumefaciens EHA 105 harboring the binary vector pCAMBIA 1301 containing the GUS gene with different concentrations of acetosyringone. The outcome of this study revealed that the frequency of callus induction was maximum on Murashige and Skoog medium (MS) fortified with B5 vitamins supplemented with 0.5 and 1 mg L-1 picloram. Regeneration of plantlets from the callus was observed on MS supplemented with 0.5 mg L-1 each of BAP and KIN and 0.1 mg L-1 NAA. In vitro bulb response was observed on MS with B5 vitamins supplemented with 2.0 mg L-1 BAP. GUS gene integration in the transgenic plants was confirmed by PCR. The maximum GUS gene expression was observed at a concentration of 150 μM acetosyringone in the transformed plants by histochemical assay. This protocol allows effective genetic transformation and regeneration system of onion via in vitro bulb development.
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