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Abd El-Aty, Abd El-Aty M,Choi, Jeong-Heui,Park, Jong-Hyouk,Shim, Jae-Han P. Parey 2007 Berliner und Münchener tierärztliche Woc Vol.120 No.5
<P>The purpose of this study was to determine whether concurrent oral administration of activated charcoal has an affect on the depletion of the residual concentrations of enrofloxacin (ENRO) in chicken breast muscles. Sixty-four broiler chickens were divided into four groups (n = 16 per group), one given a daily oral dose of enrofloxacin with feed at a dose of 10 mg/kg for 5 consecutive days (control group) and the others given the same dose of enrofloxacin simultaneously with activated charcoal at a dose rate of 0.5, 1, and 2 % of daily feed for 5 days (treatment groups). At the end of treatment, 2 hens were sacrificed at each of the sampling time points (6,12, 18,48, 72,96,120 and 144 h after completion of dosing), breast muscles were collected and analyzed. Supercritical fluid extraction and high-performance liquid chromatography methods were used to determine the enrofloxacin residue levels in chicken breast muscles.The limit of quantification (LOQ) 16.5 microg/kg, was lower than the maximum residue levels (MRL) fixed by the Commission of the European Union. For all the time periods, charcoal treatment did not affect enrofloxacin tissue concentrations except at 12 and 48 h post treatment.To our knowledge, no studies on the depletion of enrofloxacin in the presence and absence of activated charcoal in chicken muscles have been performed. Although our current understanding is incomplete, multiple dose activated charcoals may play a role in the therapy of overdose.To prove this, further investigation is warranted.</P>
EI-Aty, A.M. Abd,Shah, Syed Sher,Kim, Bo-Mee,Choi, Jeong-Heui,Cho, Hee-Jung,Yi, Hee,Chang, Byung-Joon,Shin, Ho-Chul,Lee, Kang-Bong,Shimoda, Minoru,Shim, Jae-Han 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.11
Danggui is one of the most popular herbal medicines consumed by patients in different clinical settings in Asian countries. In this study, the two major pyranocoumarin compounds extracted from the Korean Angelica gigas root decursin (DC) and decursinol angelate (DA) were examined in vitro with regard to their abilities to inhibit hepatic CYP1A1/2, CYP2D15, and CYP3A12 catalytic activities in canine liver microsomes. The two components were capable of inhibiting CYP1A1/2, CYP2D15, and CYP3A12 catalytic activities, but the potencies varied. DC and DA selectively and noncompetitively inhibited CYP1A1/2 activity, with $K_i$ values of 90.176 and $67.560{\mu}M$, respectively. On the other hand, they exhibited slight inhibitory effects on CYP2D15 and CYP3A12 with Ki values of 666.180 and $872.502{\mu}M$, 990.500 and $909.120{\mu}M$ (1'hydroxymidazolam, MDZ1'H), and 802.800 and $853.920{\mu}M$ (4-hydroxymidazolam, MDZ4H), respectively. Additionally, they showed increased inhibition after preincubation, which suggests the involvement of a mechanism-based inhibition. In sum, this in vitro data should be heeded as a signal of possible in vivo interactions. The use of human liver preparations would considerably strengthen the practical impact of the data generated from this study.
Abd El-Aty, A. M.,Lee, Go-Woon,Mamun, M. I. R.,Choi, Jeong-Heui,Cho, Soon-Kil,Shin, Ho-Chul,Shim, Jae-Han John Wiley Sons, Ltd. 2008 Biomedical chromatography Vol.22 No.3
<P>The present work reports the extraction and clean-up procedures, as well as the chromatographic conditions developed, for the determination of cafenstrole and its metabolite (CHM-03) residues in brown rice grains and rice straw using HPLC-UV detection. The method makes use of an Apollo C<SUB>18</SUB> column and acetonitrile : water : acetic acid as a mobile phase for both cafenstrole and its metabolite in rice and rice straw. Using these conditions cafenstrole and its metabolite were resolved with a retention time (R<SUB>t</SUB>) of less than 14 min. The analytes were confirmed using positive atmospheric pressure ionization LC-MS with selected ion monitoring. The average recoveries of cafenstrole were found to be 87.0–92.5 and 87.6–88.3%. However, they ranged from 81.5 to 81.6% and from 76.1 to 78.5% for cafenstrole metabolite (CHM-03), in rice grains and rice straw, respectively, with relative standard deviations ranging from 1.4 to 6.6%. The limits of detection (LODs) of both cafenstrole and its metabolite were 0.002 and 0.02 ppm and 0.025 and 0.04 ppm, respectively. Field trials with recommended or double the recommended dose revealed that the herbicide could safely be recommended for application in rice and rice straw as no residues were detected in the harvest samples. Copyright © 2007 John Wiley & Sons, Ltd.</P>
Abd El-Aty, A. M.,Kim, In-Kyung,Kim, Mi-Ra,Lee, ChiHo,Shim, Jae-Han John Wiley Sons, Ltd. 2008 Biomedical chromatography Vol.22 No.5
<P>Ginsenosides are regarded as the main active, non-volatile components of Panax ginseng (C. A. Meyer). However, throughout the long history of ginseng research, there has been virtually no report describing its volatile flavor compounds. A solvent-free procedure for the determination of volatile flavor compounds generated from fresh, white and red Panax ginseng (C. A. Meyer) using solvent-free solid injection (SFSI) coupled with gas chromatography-mass spectrometry (GC-MS) detection is described here. At no point in the SFSI technique were the extraction conditions optimized. Rather, the experimental variables including various sample preparations (fresh, oven-dried and freeze-dried), injector temperatures (100, 150, 200, 250 and 300°C), and preheating times (3, 5, 7, 10 and 15 min), were predicated on the experience of the authors. A total of 47 compounds were identified in various forms of ginseng. Among the compounds identified in the sample, fresh ginseng was characterized by a high proportion of 3-acetyl-1-(3,4-dimethoxyphenyl)-5-ethyl-4,5-dihydro-7,8-dimethoxy-4-methylene-3H-2,3-benzodiazepine (64.24%) and 23,24-dinor-3-oxolean-4,12-dien-28-oic acid (21.42%); 2-furanmethanol (20.26%) and 3-hydroxy-2-methyl-4H-pyran-4-one (17.95%) were detected as the major components in white ginseng while the main components of the red ginseng were found to be 1,2-benzenedicarboxylic acid dibutyl ester (16.27%) and 2-furanmethanol (13.82%). SFSI is a solvent-free, rapid and simple sample preparation technique based on direct vaporization. There is no dilution or contamination with solvent or its impurities and no loss of quickly eluted components was observed in the solvent peak. Copyright © 2008 John Wiley & Sons, Ltd.</P>
A. M. Abd El-Aty,Syed Sher Shah,김보미,최정휘,조희정,Hee-Yi,장병준,신호철,이강봉,Minoru Shimoda,심재한 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.11
Danggui is one of the most popular herbal medicines consumed by patients in different clinical settings in Asian countries. In this study, the two major pyranocoumarin compounds extracted from the Korean Angelica gigas root decursin (DC) and decursinol angelate (DA) were examined in vitro with regard to their abilities to inhibit hepatic CYP1A1/2, CYP2D15, and CYP3A12 catalytic activities in canine liver microsomes. The two components were capable of inhibiting CYP1A1/2, CYP2D15, and CYP3A12 catalytic activities, but the potencies varied. DC and DA selectively and noncompetitively inhibited CYP1A1/2 activity, with Ki values of 90.176 and 67.560 μM, respectively. On the other hand, they exhibited slight inhibitory effects on CYP2D15 and CYP3A12 with Ki values of 666.180 and 872.502 μM, 990.500 and 909.120 μM (1’hydroxymidazolam, MDZ1’H), and 802.800 and 853.920 μM (4- hydroxymidazolam, MDZ4H), respectively. Additionally, they showed increased inhibition after preincubation, which suggests the involvement of a mechanism-based inhibition. In sum, this in vitro data should be heeded as a signal of possible in vivo interactions. The use of human liver preparations would considerably strengthen the practical impact of the data generated from this study.
Single-dose pharmacokinetics of marbofloxacin in Egyptian buffalo (Bubalus bubalis L.) steers.
Goudah, Ayman,Abd El-Aty, Abd El-Aty M,Regmi, Nanda L,Shin, Ho-Chul,Shimoda, Minoru,Shim, Jae-Han P. Parey 2007 Berliner und Münchener tierärztliche Woc Vol.120 No.5
<P>The objective of this study was to investigate the pharmacokinetics of marbofloxacin (MAR) following intravenous (iv) and intramuscular (im) administration of a 2.0 mg/kg body weight dosage to five healthy Egyptian buffalo steers. A cross-over design was used with a washout period of 2 weeks. Blood samples were obtained at 0, 5,10,15, and 20 min and at 0.5,0.75,1,2,4,6,8,10,12,24,30 and 48 hours after marbofloxacin administration.The serum marbofloxacin concentrations were quantitated using a modified agar diffusion bioassay method. Marbofloxacin exhibited a relatively high volume of distribution at steady-state (Vdss = 1.77 Lkg), which suggests good tissue penetration, and a total body clearance (Cltot) of 0.18 L/kgxh,which is associated with a long elimination half-life (tl/2beta = 7.52 h). Marbofloxacin was rapidly absorbed at a dosage of 2.0 mg/kg after im administration with an observed maximum serum concentration (Cmax) value of 2.004 microg/mL obtained at a time to peak concentration (tmax) of 0.5 h, and an absolute bioavailability (F %) of 86.79 +/- 5.53 %.The protein-binding ranged from 22 to 24.6 % with an average of 23.4 %. In conclusion, single iv and im administered doses of marbofloxacin were well tolerated by Egyptian buffalo steers. A dosage of 2 mg/kg body weight might not be enough to treat infections caused by bacteria with minimum inhibitory concentration (MIC) at or above 0.2 microg/mL, based on the calculated area under the inhibitory concentration (AUIC).</P>