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- 저자 : Asami Kurita (검색결과 2 건)
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Hasegawa, Ai,Kurita, Asami,Hayashi, Shinji,Fukumoto, Takeshi,Sasamoto, Hamako 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2
The effects of sea salts, NaCl, KCl, $MgCl_2$, $MgSO_4$, and $CaCl_2$, on the growth of protoplast cultures of two mangrove species, Sonneratia alba and Avicennia alba, were investigated using 96-well culture plates. Plants of these two species naturally grow at the seaward side of a mangrove forest. Cotyledon protoplasts of S. alba showed halophilic nature to NaCl, KCl, and $MgCl_2$ at low concentrations (10-50 mM) when cultured in Murashige and Skoog's (MS) medium containing 0.6 M mannitol. $CaCl_2$ at a concentration higher than 25 mM was inhibitory to cell growth. On the other hand, in protoplast culture of A. alba suspension cells, which were induced from cotyledon tissues, in the modified amino acid (mAA) medium containing 1.2 M sorbitol, tolerance to NaCl, $MgCl_2$ and $MgSO_4$ were observed at a wide range of concentrations up to 400 mM. $CaCl_2$ was always inhibitory for cell divisions in A. alba, but stimulatory for spherical enlargement of cells. However, no difference in cell enlargement was observed among other salts. Similarity and difference in reactivity to salts between protoplasts and suspension cells from our previous studies were discussed in relation to the site of salt tolerance or halophilic adaptation within mangrove cells. For protoplast cultures, the site(s) for response of S. alba and A. alba are located in the cytoplasm and/or the cell membrane.
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Ai Hasegawa,Asami Kurita,Shinji Hayashi,Takeshi Fukumoto,Hamako Sasamoto 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.2
The effects of sea salts, NaCl, KCl, MgCl2,MgSO4, and CaCl2, on the growth of protoplast cultures oftwo mangrove species, Sonneratia alba and Avicenniaalba, were investigated using 96-well culture plates. Plantsof these two species naturally grow at the seaward side of amangrove forest. Cotyledon protoplasts of S. alba showedhalophilic nature to NaCl, KCl, and MgCl2 at low concentrations(10–50 mM) when cultured in Murashige andSkoog’s (MS) medium containing 0.6 M mannitol. CaCl2at a concentration higher than 25 mM was inhibitory to cellgrowth. On the other hand, in protoplast culture of A. albasuspension cells, which were induced from cotyledontissues, in the modified amino acid (mAA) mediumcontaining 1.2 M sorbitol, tolerance to NaCl, MgCl2 andMgSO4 were observed at a wide range of concentrations upto 400 mM. CaCl2 was always inhibitory for cell divisionsin A. alba, but stimulatory for spherical enlargement ofcells. However, no difference in cell enlargement wasobserved among other salts. Similarity and difference inreactivity to salts between protoplasts and suspension cellsfrom our previous studies were discussed in relation to thesite of salt tolerance or halophilic adaptation within mangrovecells. For protoplast cultures, the site(s) for responseof S. alba and A. alba are located in the cytoplasm and/orthe cell membrane.
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