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Isolation and Biochemical Characterization of Bacillus pumilus Lipases from the Antarctic
( Arifin arild Ranlym ),( Soon Ja Kim ),( Joung Han Yim ),( Antonius Suwanto ),( Hyung Kwoun Kim ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.5
Lipase-producing bacterial strains were isolated from Antarctic soil samples using the tricaprylin agar plate method. Seven strains with relatively strong lipase activities were selected. All of them turned out to be Bacillus pumilus strains by the 16S rRNA gene sequence analysis. Their corresponding lipase genes were cloned, sequenced, and compared. Finally, three different Bacillus pumilus lipases (BPL1, BPL2, and BPL3) were chosen. Their amino acid sequence identities were in the range of 92-98% with the previous Bacillus pumilus lipases. Their optimum temperatures and pHs were measured to be 40oC and pH 9. Lipase BPL1 and lipase BPL2 were stable up to 30oC, whereas lipase BPL3 was stable up to 20oC. Lipase BPL2 was stable within a pH range of 6-10, whereas lipase BPL1 and lipase BPL3 were stable within a pH range of 5-11, showing strong alkaline tolerance. All these lipases exhibited high hydrolytic activity toward pnitrophenyl caprylate (C8). In addition, lipase BPL1 showed high hydrolytic activity toward tributyrin, whereas lipase BPL2 and lipase BPL3 hydrolyzed tricaprylin and castor oil preferentially. These results demonstrated that the three Antarctic Bacillus lipases were alkaliphilic and had a substrate preference toward short- and mediumchain triglycerides. These Antarctic Bacillus lipases might be used in detergent and food industries.
Arifin, Eric,Lee, Jin-Kyu Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.2
$Ag@SiO_2@SiO_2$(FITC) nanocomposites were prepared by the simple polyol process and St$\ddot{o}$ber method. Fluorescence enhancement of fluorescein moiety (fluorescein isothiocyanate, FITC) was investigated in the presence of silver nanoparticles in $Ag@SiO_2@SiO_2$(FITC) system with varying thickness (X nm) of first silica shell. Maximum enhancement factor of 4.3 fold was achieved in $Ag@SiO_2@SiO_2$(FITC) structure with the first silica shell thickness of 8 nm and the average separation distance of 11 nm between the surface of silver nanoparticle and fluorescein moiety. The enhancement is believed to be originated from increased excitation rate of fluorescein moiety due to concentrated local electromagnetic field which was improved by interaction of light with silver nanoparticles.
( Arifin Dwi Saputro ),( Maria Paulina Gonzalez ),( Davy Van De Walle ),( Koen Dewettinck ) 한국농업기계학회 2018 한국농업기계학회 학술발표논문집 Vol.23 No.1
The use of palm sap sugar as chocolate sweetener results in chocolate with relatively high viscosity due to the presence of high moisture and/or amorphous sugar. To overcome this problem, approaches to maintain the chocolate viscosity that can be easily and feasibly applied by small-scale chocolate producers are highly required. In this work, chocolate was produced using combination of ball mill and a mixer equipped with vacuum pump. Modification of processing method using a longer duration of the vacuum process and modification of formulation by increasing lecithin concentration were challenged. To evaluate the effect of vacuum process, palm sap sugar-sweetened chocolates were produced without vacuum process, with 10 min of vacuum process and with 60 min of vacuum process during dry mixing. As reference, sucrose-sweetened chocolate was produced using 10 min of vacuum process. Ten minutes of vacuum process was the vacuum duration used during dry mixing in the previous studies. The results showed that the use of 60 min of vacuum process was most effective in evaporating moisture which resulted in a lower viscosity. Hence, 60 min of vacuum process was, then, selected for the study on production of chocolates with a higher lecithin concentrations. Palm sap sugar-sweetened chocolates produced with 60 min of vacuum process were formulated with 0.6%, 0.7% and 0.8% lecithin. As reference, palm sap sugar-sweetened chocolates with 0.4% lecithin was also produced. The result showed that the higher the lecithin concentration, the higher the Casson yield value and the lower the Casson plastic viscosity were observed. Thus, the amount of lecithin added into the chocolate should consider the application of the chocolate.
Performance Analysis of a Combined Blade Savonius Wind Turbines
Arifin Sanusi,Sudjito Soeparman,Slamet Wahyudi,Lilis Yuliati 한국유체기계학회 2017 International journal of fluid machinery and syste Vol.10 No.1
The Savonius wind turbine has a lower performance than other types of wind turbines which may attract more study focus on this turbine. This study aimed to improve wind turbine performance by combining a conventional blade with an elliptical blade into a combined blade rotor. The analysis was performed on three blade models in computational fluid dynamics (CFD) using ANSYS_Fluent Release 14.5. Then the results were verified experimentally using an open wind tunnel system. The results of the numerical simulation were similar to the experimental and showed that the combined blade rotor has better dragging flow and overlap flow than the conventional and elliptical blade. Experimental verification showed that the combined blade was to increase the maximum coefficient of power (Cpmax.) by 11% of the conventional blade and to 5.5% of the elliptical blade.
Arifin, Eric,Cha, Jinmyung,Lee, Jin-Kyu Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.8
Iron oxide (ferrihydrite, hematite, and magnetite) coated silica gels were prepared using a low-cost, easily-scalable and straightforward method as the adsorbent material for arsenic removal application. Adsorption of the anionic form of arsenic oxyacids, arsenite ($AsO^{2-}$) and arsenate ($AsO{_4}^{3-}$), onto hematite coated silica gel was fitted against non-linear 3-parameter-model Sips isotherm and 2-parameter-model Langmuir and Freundlich isotherm. Adsorption kinetics of arsenic could be well described by pseudo-second-order kinetic model and value of adsorption energy derived from non-linear Dubinin-Radushkevich isotherm suggests chemical adsorption. Although arsenic adsorption process was not affected by the presence of sulfate, chloride, and nitrate anions, as expected, bicarbonate and silicate gave moderate negative effects while the presence of phosphate anions significantly inhibited adsorption process of both arsenite and arsenate. When the actual efficiency to remove arsenic was tested against 1 L of artificial arsenic-contaminated groundwater (0.6 mg/L) in the presence competing anions, the reasonable amount (20 g) of hematite coated silica gel could reduce arsenic concentration to below the WHO permissible safety limit of drinking water of $10{\mu}g/L$ without adjusting pH and temperature, which would be highly advantageous for practical field application.
Eric Arifin,차진명,이진규 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.8
Iron oxide (ferrihydrite, hematite, and magnetite) coated silica gels were prepared using a low-cost, easilyscalable and straightforward method as the adsorbent material for arsenic removal application. Adsorption of the anionic form of arsenic oxyacids, arsenite (AsO2−) and arsenate (AsO4 3−), onto hematite coated silica gel was fitted against non-linear 3-parameter-model Sips isotherm and 2-parameter-model Langmuir and Freundlich isotherm. Adsorption kinetics of arsenic could be well described by pseudo-second-order kinetic model and value of adsorption energy derived from non-linear Dubinin-Radushkevich isotherm suggests chemical adsorption. Although arsenic adsorption process was not affected by the presence of sulfate, chloride, and nitrate anions, as expected, bicarbonate and silicate gave moderate negative effects while the presence of phosphate anions significantly inhibited adsorption process of both arsenite and arsenate. When the actual efficiency to remove arsenic was tested against 1 L of artificial arsenic-contaminated groundwater (0.6 mg/L) in the presence competing anions, the reasonable amount (20 g) of hematite coated silica gel could reduce arsenic concentration to below the WHO permissible safety limit of drinking water of 10 μg/L without adjusting pH and temperature, which would be highly advantageous for practical field application.
Estimation of Cloud Motion Vector Based on the Optical Flow Method to Forecast Cloud Movement
Rial Arifin,Hyunjin Lee 대한설비공학회 2019 대한설비공학회 학술발표대회논문집 Vol.2019 No.-
One important step in forecasting short-term solar irradiance is to predict the movement of clouds. The optical flow method can be used for tracking and estimating the motion of cloud using images. It assumes that a feature point maintains constant brightness across the images. The optical flow method is comprised of a process of calculating the motion between two or more images that can be used to extract the cloud motion vector (CMV) from each pixel of an image. The cloud motion is predicted by using CMV’s data from the previous cloud movements. Using Korea Meteorological Agency (KMA) satellite images, we predicted the orientations of 15 minutes ahead cloud movement and compared them to the actual cloud movement. The errors of forecast cloud movement were evaluated in terms of the mean absolute error (MAE) and the root mean square error (RMSE).