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RISS 인기검색어
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- 저자 : Anwar Shahzad (검색결과 2 건)
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Shahzad Anwar,Ahmad Naseem,Anis Mohammad The Korean Society of Plant Biotechnology 2006 Plant molecular biology and biotechnology research Vol.8 No.1
An efficient protocol for in vitro multiple shoot bud induction and plant regeneration from mature green cotyledon derived callus tissues of Acacia sinuata has been developed. Callus formation occurs at all the concentrations of thidiazuron (TDZ) in Murashige and Skoog's (MS) medium, but 0.6 ${\mu}M$ proved to be the best with maximum callus formation frequency. Supplementation of TDZ in combination with indole-acetic acid (IAA) in MS media accelerates shoot bud organogenesis in differentiating callus tissues with 60-70% conversion of shoot buds into shoot Most efficient shoot organogenesis was recorded when TDZ induced calli were subcultured at different concentrations of 6-benzyla-denine (BA). Optimum shoot bud induction and plant regeneration from callus was achieved when 0.6 ${\mu}M$ (TDZ) induced calli were subcultured at 3.0 ${\mu}M$ (BA) where $16.6{\pm}0.74$ shoots/unit callus on obtained. Rooting in in vitro differentiated shoots was achieved when transferred to medium containing different concentration of indole-3-butyric acid (IBA) in full & half strength MS medium. The well rooted plantlets were hardened and transferred to net house with 90% survival rate.
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Anwar Shahzad,Aastha Sahai 한국산림과학회 2014 Forest Science And Technology Vol.10 No.3
The present study reports variation in in vitro germination responses between two genotypes of Balanites aegyptiaca (L.)Del. an endangered agroforestry species. Fruits were collected from arid and semi-arid zones of Jodhpur (J) andAhmedabad (A) respectively. Mature seeds of both the germplasms were isolated and subjected to in vitro germination onMurashige and Skoog medium supplemented with gibberellic acid (GA). Marked differences were observed ingermination frequency of both genotypes at variable concentrations of GA. Whereas ‘A’ acquired 100% germinationfrequency on medium containing 1mM GA, ‘J’ required 2.5mM GA. Morphogenic variations for seedling traits were alsorecorded in relation to genotype for shoot, internode and root length along with number of nodes/shoot and leaf size andcolor. ‘A’ seedlings appeared healthy as compared to ‘J’ seedlings. These morphogenic variations were studied incorrelation with the native environmental conditions of both germplasms. In vitro germinated seedlings were hardened andacclimatized in ex situ conditions and survivability was checked during in vitro hardening and ex situ acclimatization inrelation to required humidity and shade density respectively.
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