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        Genotype-dependent responses during in vitro seed germination and establishment of Balanites aegyptiaca (L.) Del. – an endangered agroforestry species

        Anwar Shahzad,Aastha Sahai 한국산림과학회 2014 Forest Science And Technology Vol.10 No.3

        The present study reports variation in in vitro germination responses between two genotypes of Balanites aegyptiaca (L.)Del. an endangered agroforestry species. Fruits were collected from arid and semi-arid zones of Jodhpur (J) andAhmedabad (A) respectively. Mature seeds of both the germplasms were isolated and subjected to in vitro germination onMurashige and Skoog medium supplemented with gibberellic acid (GA). Marked differences were observed ingermination frequency of both genotypes at variable concentrations of GA. Whereas ‘A’ acquired 100% germinationfrequency on medium containing 1mM GA, ‘J’ required 2.5mM GA. Morphogenic variations for seedling traits were alsorecorded in relation to genotype for shoot, internode and root length along with number of nodes/shoot and leaf size andcolor. ‘A’ seedlings appeared healthy as compared to ‘J’ seedlings. These morphogenic variations were studied incorrelation with the native environmental conditions of both germplasms. In vitro germinated seedlings were hardened andacclimatized in ex situ conditions and survivability was checked during in vitro hardening and ex situ acclimatization inrelation to required humidity and shade density respectively.

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