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- 저자 : Anna Herman (검색결과 3 건)
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Anna Ratz-Łyko,Anna Herman,Jacek Arct,Katarzyna Pytkowska 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.4
The antioxidant and antimicrobial activities ofOenothera biennis, Borago officinalis, and Nigella sativaseedcake extracts were determined. Seedcakes weresubjected to extraction using 50% ethanol (v/v) during 48 hat a solvent temperature of 50oC. The total polyphenol andflavonoid contents were assayed using UV spectrophotometryand the antioxidant activities were determinedusing DPPH and ABTS testing methods. Extracts werealso analyzed for polyphenol content using HPLC. In vitroantimicrobial activities were evaluated using the discdiffusion method and resazurin testing. The ranking of thepolyphenol contents and the antioxidant activities ofexamined seedcake extracts was O. bienis>B.officinalis>N. sativa. The highest antimicrobial activity in both testswas for N. sativa seedcake extracts against Pseudomonasaeruginosa (MIC=300 mg/mL), Escherichia coli (MIC=300 mg/mL), and Staphylococcus aureus (MIC=700 mg/mL).
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Endotoxin-induced inflammation disturbs melatonin secretion in ewe
Andrzej Przemyslaw Herman,Karolina Wojtulewicz,Joanna Bochenek,Agata Krawczynska,Hanna Antushevich,Bartosz Pawlina,Marlena Zielinska-Gorska,Anna Herman,Katarzyna Romanowicz,Dorota Tomaszewska-Zaremba 아세아·태평양축산학회 2017 Animal Bioscience Vol.30 No.12
Objective: The study examined the effect of intravenous administration of bacterial endotoxin—lipopolysaccharide (LPS) —on the nocturnal secretion of melatonin and on the expression of enzymes of the melatonin biosynthetic pathway in the pineal gland of ewes, taking into account two different photoperiodic conditions: short-night (SN; n = 12) and long-night (LN; n = 12). Methods: In both experiments, animals (n = 12) were randomly divided into two groups: control (n = 6) and LPS-treated (n = 6) one. Two hours after sunset, animals received an injection of LPS or saline. Blood samples were collected starting one hour after sunset and continuing for 3 hours after the treatment. The ewes were euthanized 3 hours after LPS/saline treatment. The concentration of hormones in plasma was assayed by radioimmunoassay. In the pineal gland, the content of serotonin and its metabolite was determined by HPLC; whereas the expression of examined genes and protein was assayed using real-time polymerase chain reaction and Western Blot, respectively. Results: Endotoxin administration lowered (p<0.05) levels of circulating melatonin in animals from LN photoperiod only during the first hour after treatment, while in ewes from SN photoperiod only in the third hour after the injection. Inflammation more substantially suppressed biosynthesis of melatonin in ewes from SN photoperiod, which were also characterised by lower (p<0.05) cortisol concentrations after LPS treatment compared with animals from LN photoperiod. In the pineal gland of ewes subjected to SN photoperiod, LPS reduced (p<0.05) serotonin content and the expression of melatonin biosynthetic pathway enzymes, such as tryptophan hydroxylase and arylalkylamine-N-acetyltransferase. Pineal activity may be disturbed by circulating LPS and proinflammatory cytokines because the expression of mRNAs encoding their corresponding receptors was determined in this gland. Conclusion: The present study showed that peripheral inflammation reduces the secretion of melatonin, but this effect may be influenced by the photoperiod.
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Amarila Malik,Melya Leonita Sutantyo,Indah Hapsari,Anna Veronika Sinurat,Euis Maras Purwati,Mahdi Jufri,Herman Suryadi 한국약제학회 2016 Journal of Pharmaceutical Investigation Vol.46 No.5
Pharmaceutical dosage forms containing gelatin are recently of high attention in regard to its porcine content. In Muslim countries, halal products assurance is strictly regulated, not only for food but also for pharmaceutical products. This study aimed to develop gelatin identification and verification method in capsule shells by performing simple simultaneous protein- and nucleic acid based-identification methods. Identification and verification performing polyacrylamide gel electrophoresis (PAGE)—coupled with principle component analysis, and polymerase-chain-reaction (PCR)-restriction fragment length polymorphism (RFLP) were carried out. Gelatin was obtained by extracting the capsules using cold acetone; the precipitate was used directly for PAGE, and was used as well for DNA extraction. The results showed that the porcine gelatin reference showed 12 major bands of peptide profile, with predominant bands at ±200 and ±100–135 kDa on 8 % Tris-glycine gel, while bovine gelatin only showed four major bands. Further, PCR-RFLP result showed two specific bands of the porcine gelatin DNA at 228 and 131 bp, whereas the bovine gelatin DNA bands occurred at 316 and 44 bp. The results demonstrated that the capsule shell samples used in this study contained the following: sample 1 was verified contain porcine gelatin, whereas the three other samples identified contain bovine gelatin. Although the simultaneous approaches seemed need to be improved, it is crucial to maintain their low cost and simple protocol.
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