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Functional Genomics for Resistance Mechanism
Pereira, Andy 영남대학교 마늘연구소 2001 의성군 ·영남대학교 관 ·학협동, 협약 조인식 및 심포지움 Vol.2001 No.-
Genomics can provide new tools and information that are applicable for breeding of crop resistance to various biotic and abiotic stresses. The genome sequence of the model plant Arabidopsis reveals the structure of about 25,000 genes, most of unknown function. The sequence of the model monocot plant rice is also progressing rapidly, with about one-third available in public databases and for many important crop plants EST sequence resources are available. Functional genomics strategies are being employed to discover gene functions using mutants, expression analysis, and metabolic profiling. As genome redundancy limits a mutation approach to studying gene functions we employ a novel method of gene function discovery using Activation tagging that generates overexpression phenotypes that help to define gene function. Specific screens for gain-of-function mutants are being used to select plants differing in stress and biotic interactions. This will uncover gene functions involved in various general resistance mechanisms so that resistance management using different mechanisms can be done. To identify specific genes involved in resistance to pathogens, map based cloning approaches are being followed. Genes discovered can be employed through a transgenic approach or using molecular markers for selection using sequence polymorphisms like SNPs.
Rice mutant resources for gene discovery
Hirohiko Hirochika,,Emmanuel Guiderdoni,An, Gyn-heung,Yue-ie Hsing,Eun, Moo-Young,Han, Chang-deok,Narayana Upadhyaya,Hei Leung,Srinivasan Ramachandran,Qifa Zhang,Andy Pereira,Venkatesan Sundaresan Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
With the completion of genomic sequencing of rice, rice has been firmly established as a model organism for both basic and applied research. The next challenge is to uncover the functions of genes predicted by sequence analysis. Considering the amount of effort and the diversity of disciplines required for functional analyses, extensive international collaboration is needed for this next goal. The aims of this review are to summarize the current status of rice mutant resources, key tools for functional analysis of genes, and our perspectives on how to accelerate rice gene discovery through collaboration.