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A Potential Role of ppGalNAc-T13 in Neural Differentiation
Yingjiao Xu,Wenjie Pang,Aidong Shan,Wei Li,Yan Zha 한국당과학회 2012 한국당과학회 학술대회 Vol.2012 No.1
Glycosylation in the nervous system is thought to play a particularly important and dynamic role in both vertebrates and invertebrates. Mucin-type O-glycosylation is initiated by a large family of UDP- GalNAc:polypeptideα-N-acetylgalactosaminyltransferases (ppGalNAc-Ts, EC 2.4.1.41) which contains 20 members. ppGalNAc-T13 is one of the essential isoform, and has been found highly and restrictively expressed in the brain and neurons [1]. In this study, we found that the mRNA and protein expression of ppGalNAc-T13 was dramatically increasing during the all-trans retinoic acid (ATRA) induced neural differentiation of embryonic carcinoma P19 cells, mouse brain development and primary neuronal cultures. ppGalNAc-T13 was localized in axons of the neurons derived from P19 cells and the O-glycosylation level was simultaneously increased during the ATRA induced neural differentiation. Furthermore, up-regulation of ppGalNAc-T13 induced the formation of lamellipodia and a higher O-glycosylation level. These results imply that the high expression of ppGalNAc-T13 in neurons may play a regulatory role in the neural differentiation. Study to explore the roles of ppGalNAc-T13 in regulating neural differentiation is under way.
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Guan Yongchao,Zhang Meng,Wang Yingda,Liu Zhongzhuo,Zhao Zelin,Wang Hong,An Dingjie,Qian Aidong,Kang Yuanhuan,Sun Wuwen,Shan Xiaofeng 한국미생물학회 2022 The journal of microbiology Vol.60 No.12
Aeromonas veronii is a pathogen which can induce diseases in humans, animals and aquatic organisms, but its pathogenic mechanism and virulence factors are still elusive. In this study, we successfully constructed a mutant strain (ΔascP) by homologous recombination. The results showed that the deletion of the ascP gene significantly down-regulated the expression of associated effector proteins in A. veronii compared to its wild type. The adhesive and invasive abilities of ΔascP to EPC cells were 0.82-fold lower in contrast to the wild strain. The toxicity of ΔascP to cells was decreased by about 2.91-fold (1 h) and 1.74-fold (2 h). Furthermore, the LD50 of the mutant strain of crucian carp was reduced by 19.94-fold, and the virulence was considerably attenuated. In contrast to the wild strain, the ΔascP content in the liver and spleen was considerably lower. The titers of serum cytokines (IL-8, TNF-α, and IL-1β) in crucian carp after the infection of the ΔascP strain were considerably lower in contrast to the wild strain. Hence, the ascP gene is essential for the etiopathogenesis of A. veronii TH0426.
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