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RISS 인기검색어
- 검색키워드
- 저자 : 李麟九 (검색결과 2 건)
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朴商玉,李浩俊,李麟九 韓國自然史博物學會 1981 生物과 自然 Vol.11 No.1
Analyzing the water quality biologically by the biotic index(β) of Beck-Tsuda method, for six sites of the water system of Sincheon River which reaches a length of 22km and flows through the Taegu city and Gachang-myun in Dalseong-gun, and extends to the Gumho-river on the lower, it is summarized as follows: ① An indicator species collected from the water system of Sincheon River comprises 37 species and all is tolerant species to water pollution. ② The Sincheon River mainly was polluted with sewage from the city, with wastes from the factory surviving the city and with industrial wastes or products. ③ The water of 3 sites, Naengcheon, Gachang-bridge, and Jungdong-bridge were slightly polluted β-mesosaprobic zone. Those of 2 sites, Dongsin-bridge and Kyungdae-bridge were polluted α-mesosaprobic zone. But the lower Sincheon River was severely polluted polysaprobic zone. ④ The map showing a class of water quality analyzed biologically was also prepared for each site of the water system of Sincheon River. ⑤ The pH of water running the water system of Sincheon River was alkalescent. ⑥ An advantage of biological analysis of water quality was discussed.
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Heterologous plasmid DNA 의 transformation에 있어서의 細胞膜 蛋白質의 기능에 對하여
徐正塤,洪淳德,李麟九,金英浩 慶北大學校 1983 論文集 Vol.36 No.-
Terramycin resistance plasmid DNA and Streptomycin resistance plasmid DNA have been prepared from Streptomyces rimosus and E. coli KPM 105, respectively, by phenol extraction of lysozyme-lysed cells. And the plasmid DNA was introduced into B. subtilis KPM 73, B. subtilis BD 224, B. subtilis 110, B. subtilis RM 125, B. amyloliquef aciens and B. megaterium IAM B425 by transformation. The variable characters affecting heterologous plasmid transformation, and the functions of competence factor and binding factor existing in the recipient cell surface were futhermore studied. Terramycin resistance plasmid was well transformed into Bacillus subtilis KPM 73 and B. subtilis RM 125, and Streptomycin resistance plasmid was well transfomed into B. subtilis BD 224, B. subtilis 110 and B. megaterium IAM B 425 at high frequency. The high frequency of plasmid transformation was obtained at 4 hr of incubationin growth medium. 20 to 50 min in competence medium, and the optimal pH and temperature for competence were 7.0 and 20C to 30C, respectively. The transformation of B. subtilis KPM 73 reached a maximum level after 20 min of exposure to DNA, B. subtilis BD 224 after 30min, and B. subtilis RM 125 after 10 min. Ca, Cu, Fe, Mg and Mn ions increased the transformation frequency of B. subtilis KPM 73 but Hg, Zn and Co ions decreased the transformation frequency. Ca ions were required for transformation of E. coli C600 and tranrsformation frequency was increased 20 times by addition of 75mM CaCl_2. The competence development of recipient cell was enhanced by addition of lysozyme in the competence medium and lysozyme was more affective in the early stage of competence development than the late stage of competence development, in which the spontaneous competence development reached maximum level. In sight of these observations, competence factor would seem to be a kind of cell wall lytic enzyme such as lysozyme. when the protoplasts of B. subtilis KPM 73 were transformed by terramycin resistance plasmid, the protoplasts were successfully transformed and the transformation frequency was 10^-2. The competent cells were treated with small amount of protein-digesting enzyme such as trypsin, and then exposed to transforming plasmid DNA. The recipient cells that were treated with trypsin were transformed 10 to 100 folds lower than non-trypsin-treated control cells. These results show that the binding factor must be a protein or at least a complex containing protein that is essential to it's activity. And external protein such as bovine albumin was effective on transformation of trypsin treated cell, suggesting that the cell surface-located binding factor did not have a specificity and the binding factor could be replaced by external substance such as bovine albumin. Terramycin resistance plasmid extracted from Ter^R transformant of B. subtilis KPM 73 was well transformed into B. subtilis KPM 73. And the electrophorsis pattern of Ter^R plasmid extracted from Ter^R transformant of B. subtilis KPM 73 was equal to that from St. rimosus.
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