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골수간엽줄기세포와 폴리글리콜라이드 부직포지지체를 이용한 조직공학적 척추신경재생
김선경 ( Sun Kyung Kim ),홍금덕 ( Keum Deok Hong ),장지욱 ( Ji Wook Jang ),이상진 ( Sang Jin Lee ),김문석 ( Moon Suk Kim ),강길선 ( Gil Son Khang ),이일우 ( Il Woo Lee ),이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2004 조직공학과 재생의학 Vol.1 No.2
Bone marrow stromal stem cells (BMSCs) were harvested from the femurs and tibias of adult female Fischer rat. BMSCs were suspended at 2 × 107 cell/mL in a 25% Pluronic F127 (PF127) solution and then polyglycolide (PGA) nonwoven was saturated with 50 μl PF127 solution. The ability of the PGA/PF127 with BMSCs to be integrated and to promote nerve regeneration in the transected rat spinal cord was investigated. Fischer rat received an implant consisting of the BMSCs suspended in the PGA/PF127 in the gap (T8~T9;~5 mm) created by the spinal cord resection. For histological evaluation, the implants were removed after 4 and 8 weeks. Thin sections were cut from paraffin embedded tissue and histological sections were stained hematoxylin and eosin staining. Motor functional outcome measurements using the Basso-Beattie-Bresnehan (BBB) score performed weekly to 8 weeks postinjury. It was observed that the effects of the PGA/PF127 with BMSCs on neuroinduction (Group III, tissue engineered) are stronger that PGA/PF127 without BMSCs (Group II, matrix control) and blank control (Group I). In conclusion, these results suggest that BMSCs may have an important role for spinal cord regeneration of tissue engineering area.
골수간엽줄기세포 및 다공성 지지체를 이용한 조직공학적 척추신경재생
김선경 ( Sun Kyung Kim ),홍금덕 ( Keum Deok Hong ),김순희 ( Soon Hee Kim ),김문석 ( Moon Suk Kim ),강길선 ( Gil Son Khang ),이일우 ( Il Woo Lee ),한창환 ( Chang Whan Han ),이혜경 ( Hea Kyung Lee ),이해방 ( Hai Bang Lee ) 한국조직공학과 재생의학회 2005 조직공학과 재생의학 Vol.2 No.2
Bone marrow stromal cells (BMSCs) were harvested from the femurs and tibias of adult female Fischer rat. BMSCs were suspended at 2×106 cell/mL in poly(L-lactide-co-glycolide) (PLGA) which was saturated with 0.5 ml methylene chloride. The ability of the PLGA with BMSCs to be integrated and to promote nerve regeneration in the transected rat spinal cord was investigated. Fischer rat received an implant consisting of the BMSCs suspended in the PLGA in a 5 mm gap (at position; T8~T9) created by the spinal cord resection. For histological evaluation, the implanted regions were removed after 4 and 8 weeks. Thin sections were cut from paraffin embedded tissue and histological sections were stained hematoxylin and eosin staining. Motor functional outcome measurements using the Basso-Beattie-Bresnehan (BBB) score performed weekly during 8 weeks of post-injury. It was observed that the effects of the PLGA with BMSCs on neuroinduction (Group III, with scaffold, cell & cytokine) are stronger that PLGA without BMSCs (Group II, with scaffold only) and blank model (Group I). In conclusion, these results suggest that BMSCs and BDNF may have an important role for spinal cord regeneration of tissue engineering.