http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
양은진(Eun-Jin Yang),현광희(Kwang Hee Hyun),최해리(HaeRi Choi),김승영(Seung-Young Kim),현창구(Chang-Gu Hyun) 한국생물공학회 2018 KSBB Journal Vol.33 No.3
The anti-inflammatory activity and ignorable toxicity of Rumex acetosella extract (J9) were investigated and confirmed by measuring the expression level of NO, PGE2, and pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) in lipopolysaccharide (LPS)- and J9-treated in RAW264.7 macrophages. Relative to a LPS-only-treated group, the inhibition effect of J9 on the production of NO, PGE2, pro-inflammatory cytokines was increasing across J9-treated samples but the cell viabilities stayed constant. We also exhibited that the iNOS expression level in the J9-treated samples was dose-dependently decreased relative to its untreated control and the elevated expression level of COX-2 seemed to be equal across samples treated with LPS alone or in combination with J9.
김민진(Min-Jin Kim),현광희(Kwang Hee Hyun),김지혜(Ji-Hye Kim),한동환(Dong Hwan Han),김승영(Seung-Young Kim),이남호(Nam Ho Lee),현창구(Chang-Gu Hyun) 한국생물공학회 2017 KSBB Journal Vol.32 No.3
To identify the natural and novel cosmeceutical ingredients which have whitening function, we examined the effects of tyrosinase activity and melanin production on halophyte Limonium tetragonum (Thunb.) A. A. Bullock extracts. As a result, the extracts showed whitening effect with no cytotoxicity. Melanin contents of B16F10 melanoma cells were decreased in a dose-dependent manner at 50, 100, 200 μg/mL treatment of the extracts. In tyrosinase activity inhibition test, L. tetragonum (Thunb.) A. A. Bullock extracts showed decreased tyrosinase activity as the concentration of α-MSH was increased. Furthermore, it was observed that the tyrosinase and MITF expression was significantly downregulated by adding L. tetragonum (Thunb.) A. A. Bullock extracts in α-MSH, a melanogenesis inducing material, treated B16F10 melanoma cells. These results indicate that L. tetragonum (Thunb.) A. A. Bullock extract might be an effective whitening agent by inhibit melanin formation.
애기수영, 방가지똥 및 암대극 추출물이 Melanoma Cell에서 멜라닌 합성에 미치는 영향
김민진(Min-Jin Kim),김서연(Seoyeon Kim),현광희(Kwang Hee Hyun),김덕수(Duk Soo Kim),김승영(Seung-Young Kim),현창구(Chang-Gu Hyun) 한국생물공학회 2017 KSBB Journal Vol.32 No.3
In this study, we investigated the effect of Rumex axetosella, Sonchus oleraceus and Euphoibia jolkini extracts on tyrosinase activity and melanin production as natural products of whitening functional cosmetics. To measure the melanin production, 50, 100, 200 μg/mL of Rumex axetosella, Sonchus oleraceus and Euphoibia jolkini extracts were treated on α-MSH treated B16F10 melanoma cells, respectively. Melanin contents in α-MSH treated B16F10 melanoma cells were decreased by 41.5, 51.11, and 61% in 200 μg/mL treatment compared to none treatment, respectively. In addition, the intracellular tyrosinase activity was decreased after treatments with all extracts. Furthermore, 100 μg/mL of Euphoibia jolkini extract was decreased 81.5% of melanin production in B16F10 melanoma cells. When the three extracts were compared, Euphoibia jolkini extract was considered to be the most functional material for whitening effect.
김민진(Min-Jin Kim),김서연(Seoyeon Kim),현광희(Kwang Hee Hyun),김덕수(Duk Soo Kim),김승영(Seung-Young Kim),현창구(Chang-Gu Hyun) 한국생물공학회 2017 KSBB Journal Vol.32 No.3
Melanin is one of the most important factors affecting skin color. Melanogenesis is the bioprocess of melanin production by melanocytes in the skin and hair follicles and is mediated by several enzymes, such as tyrosinase, tyrosinase related protein (TRP)-1, and TRP-2, MITF. In this study, we investigated the effect of Artemisia fukudo Makino extracts on tyrosinase activity and melanin production as natural products of whitening functional cosmetics. Melanin content in murine B16F10 melanoma cells were decreased by Artemisia fukudo Makino extracts in a dose-dependently. In addition, the inhibition of tyrosinase activity of Artemisia fukudo Makino extracts showed to decrease tyrosinase activity as the concentration of α-MSH was increased. Furthermore, western blot analysis revealed that Artemisia fukudo Makino extracts significantly downregulated the expression of tyrosinase, TRP-1 which treat of α-MSH–induced melanogenesis in murine B16F10 melanoma cells. As a result, Artemisia fukudo Makino extract showed functionalities as an effective whitening agent to inhibit melanin formation.