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박종진,함형배,이민웅 한국균학회 1980 韓國菌學會誌 Vol.8 No.3
Poria cocos, a parasite on Pinus densiflora was studied for its optimum growing condition from May 1, 1979 to November 15, 1980. 1) The optimum pH value was 5.0, and it had poor growth below pH 3. 0 and no growth above pH 7. 0. 2) The optimum temperatures were 25∼27℃, and it had poor growth below 5℃. 3) On Bobbins and Herrey's solid media, malt extract(diameter of colony at 2% of the above material was 90㎜) and tomato extract(at 8% was 90㎜) gave the best growth. 4) By Badcock method, the best growth was obtained in P.D.A. supplemented with accelerator 5% of the above material of liquid media(85㎜ in diameter of colony) and malt extract 2% of P.D.A. added with accelerator 5% of them of liquid media (410㎎ of hyphae of dry weight) but the growth rate was poor in the media of wood extract agar supplemented with accelerator 5% of the above material giving 30mm diameter of the colony. 5) The growth on Bobbins basal medium supplemented with Quercus accutissima extract showed 305.3㎎ of hyphae of dry weight and Robinia pseudoacasia was supplemented with it showed 256.3㎎ of them. 6) The best growth was obtained in Jennison basal medium supplemented with L-asparagine showing 44.3㎎ of hyphae of dry weight.