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< 구두-D-03 > LED (Light Emitting Diode) light가 Cordyceps militaris 균사체의 생장 및 cordycepin 함량 증대에 미치는 영향
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.1
Mushroom is an abundant source of a wide range of useful natural products with biological activities. Because field cultivation of ushrooms takes a long time to grow and low productivity of bioactive compounds, submerged cultivation of mushrooms is viewed as a promising alterative for producing valuable substances. Cordyceps militaris (C. militaris), a caterpillar-shaped Chinese traditional medicinal mushroom, is usage as a crude drug, it has been extensively used as folk tonic food or invigorant since ancient times. This mushroom produces an important bioactive compound, cordycepin (3′-deoxyadenosine), which is a nucleoside analogue. Cordycepin is reported to possess many interesting biological and pharmacological activities, including immunological stimulating, anti-cancer, anti-virus and anti-infection activities. Previous work reported the isolation of cordycepin from liquid culture medium of C. militaris and its pharmacological functions. But, as far as we know, there is limited knowledge about the LED (Light Emitting Diode) condition for cordycepin production by C. militaris. In this paper, the effects of LED were focused in order to improve the cordycepin production by submerged cultivation of C. militaris. For this experiment, mycelial cultivation was performed in a shaking incubator at 24 ℃, 100 rpm for 5 days, and the medium was sabouraud dextrose broth (pH 5.6). The LED light mixed red and green, LED light mixed red and blue and LED light mixed green and blue were used for the LED, and dark culture and fluorescent lamp were used as the control. This results, C. militaris showed the highest mycelial weight when green light was irradiated, on the other hand, when irradiated with blue light, the content of cordycepin is about 7 times higher than that of fluorescent lamp in the cultures of C. militaris. In addition, the highest content of cordycepin was observed when irradiated for 6 h/day for 3 days. Interestingly, mycelial weight and cordycepin content were inversely related. The information obtained is considered fundamental and useful to the development of C. militaris cultivation process for efficient production of cordycepin on a large scale.
ICT 기술을 이용한 토종다래 재배지의 토양환경인자 분석 및 결정트리 적용
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2019 한국목재공학회 학술발표논문집 Vol.2019 No.2
The other edible and very promising Actinidia species is Actinidia arguta (Siebold & Zucc.) Planch. ex Miq., also known as kiwiberry, hardy kiwi, baby kiwi, or mini kiwi. This exotic species is very interesting and promising given the horticultural advantages it has over kiwifruit, especially its high frost hardiness (down to -30 ℃ in midwinter) and relatively short vegetation period. To the best of our knowledge, there have been few experiments studying the effect of soil properties on mini kiwifruit productivity as well as the sugar content of A. arguta fruit. The variety of cultivar and expansion in arable farming has emphasized a need for more specific information related to factors that affect A. arguta productivity and sugar content of the fruit in the region. We analyzed various soil properties at experimental sites in South Korea. A Pearson’s correlation analysis was performed between the soil properties and sugar content or productivity of A. arguta. Further, a decision tree was used to determine the optimal soil conditions. The difference in the fruit size, sugar content, and productivity of A. arguta across sites was significant, confirming the effects of soil properties. The decision tree analysis showed that a soil C/N ratio of over 11.49 predicted a sugar content of more than 7 °Bx at harvest time, and soil electrical capacity below 131.83 μS/cm predicted productivity more than 50 kg/vine at harvest time. Our results present the soil conditions required to increase the sugar content or productivity of A. arguta, a new A. arguta cultivar in South Korea.
Zanthoxylum schinifolium에서 추출한 hydrosol이 항 알레르기 염증에 미치는 영향
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),이동환 ( Dong Hwan Lee ),최민서 ( Min Seo Choe ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1
Zanthoxylum schinifolium is a deciduous shrub, which grows abundantly in Korea, China, Taiwan, Manchuria, and Japan. Currently, still most of Z. schinifolium is being extracted with essential oils, and then chemical components are analyzed. Further, various pharmacological effects have been studied. However, the yield of oil separated from the fruit or leaves of Zanthoxylum is significantly low (below 10%). Hydrosol, also called hydrolate or oral water, is a hydrophilic fraction that can be obtained during the essential oil extraction process as a byproduct. It has a pleasant aroma and is recognized for commercial applications. However, limited reports are available on this aspect of Z. schinifolium. This study investigated for the first time the analysis by GC/MS and the potential anti-inflammatory activities of hydrosol, specifically those of the branch from Z. schinifolium. A total of 102 components were identified in the hydrosol of the branch. The main chemical components of the hydrosol were estragole (50.86 %) and camphor (30.68 %). Our findings showed that hydrosol obtained from branches inhibited β-hexosaminidase release in a dose-dependent manner in RBL-2H3 mast cells, and confirmed that hydrosol at the tested concentrations did not show cytotoxicity to RBL-2H3 cells by MTT assay. Additionally, we found that hydrosol obtained from the branches significantly inhibited intracellular active oxygen (ROS) and nitric oxide (NO). Consistently, the SNARE protein of SNAP23, syntaxin4, VAMP7, and VAMP8 were also remarkably decreased by hydrosol treatment. Further investigation identified camphor and estragole, the main chemical components of hydrosol, to downregulate LPS-induced phosphorylation of the SNARE protein. Based on these findings, hydrosol obtained from the branch of Z. schinifolium is suggested to have therapeutic potential for allergic inflammatory diseases.
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.2
Zanthoxylum schinifolium Sieb. et Zucc. (syn. Fagara schinifolia Engler) was studied for its potential anti-inflammatory properties. The hydrosol extract prepared from the Z. schinifolium branch was analyzed by gas chromatography/mass spectrometry. Here, five main chemical components were identified in the hydrosol of the branches of this shrub. The main chemical compounds in the branch inhibited both an Immunoglobulin E (IgE)-antigen complex and a dinitrophenyl-bovine serum albumin (DNP-BSA)-induced β-hexosaminidase release in a dose-dependent manner in RBL-2H3 mast cells, and at the tested concentrations did not show cytotoxicity to RBL-2H3 cells. Moreover, hydrosol obtained from the branch substantially inhibited a lipopolysaccharide (LPS) induced overproduction of intracellular active oxygen (ROS) and nitric oxide (NO). Consistently, the soluble N-ethylmaleimide-sensitive factor-attachment protein receptor (SNARE) proteins of SNAP23, syntaxin4, VAMP7, and VAMP8 were remarkably decreased through hydrosol treatment. Hydrosol suppressed the activation of SNARE proteins in DNP-BSA-stimulated RBL-2H3 cells and inhibited ROS and NO in LPS-stimulated RAW264.7 cells. Camphor and estragole are the main chemical components of hydrosol and downregulate the LPS-induced phosphorylation of the SNARE proteins. The hydrosol obtained from the branch of Z. schinifolium has therapeutic benefits for allergic inflammatory diseases.
산초나무 열매 essential oil의 DNP-BSA로 유도된 β-헥소사미니다제 방출 억제 효과
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.2
Plant-derived compounds have been reported to possess anti-inflammatory abilities contained inhibited β-hexosaminidase, ROS and NO release. Essential oils are natural volatile complex compounds that are characterized by a strong scent and produced by aromatic plants as various plant-derived compounds. The essential oil extracted from Zanthoxylum coreanum Nakai (Z. coreanum) has various functional properties; however, little information is available regarding its anti-allergic inflammatory. A total of 17 compounds were detected in Z. coreanum oil, and the main component was estragole (50.86%). The tested Z. coreanum oil and estragole statistically inhibited the release of β-hexosaminidase induced by antigen stimulation in RBL-2H3 cells. This Z. coreanum oil and estragole may stimulate the secretion of active oxygen (ROS) or nitric oxide (NO) which are considered to involved in anti-inflammatory events. Moreover, it is suggested that Z. coreanum oil and estragole may negatively control the production of SNARE proteins (VAMP7) at the transcriptional and translational levels in common. These results demonstrate that Z. coreanum oil and its major component, estragole, possess potent anti-inflammatory abilities that are coupled with antioxidant properties.
동충하초 균사체 및 동충하초 균사체로부터 분리된 코디세핀의 항암 활성 평가
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),이동환 ( Dong Hwan Lee ),최민서 ( Min Seo Choe ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2021 한국목재공학회 학술발표논문집 Vol.2021 No.1
Various medicinal fungi have been traditionally used in many countries for health maintenance, as well as for the prevention and treatment of various diseases. Dong-Chong-Xia-Cha in Chinese, which translates as “winter worm and summer grass”, is an entomogenous fungus that colonized the larvae or pupae of insect. Although various biological activities of crude extracts have been reported, the precise active compounds responsible for the anticancer activities of cultured Cordyceps are not well defined. Therefore, as part of our study program to identify potential anticancer agents from traditional medicines, we used cordycepin isolated from Cordyceps militaris obtained by submerged cultivation. The aim was to isolate the pure cordycepin from the extracts of Cordyceps militaris and evaluate its anticancer properties. Silica gel column chromatographic purification of Cordyceps militaris extracts resulted in the isolation of cordycepin. The isolated cordycepin and crude cordyceps were examined for their anti-proliferative effects on human cancer cell lines, HCT, MC5-7, U-87MG, AGS, A549, and MCF-7 cells were analyzed. The isolated cordycepin displayed potent growth inhibition on HCT, MC5-7, U-87MG, AGS, A549, and MCF-7 cells with IC50 values of 86.16, 28.13, 282.40, 78.41, 137.40, and 216.40 μg/ml, respectively. A similar inhibitory trend in these cancer cells was observed for crude cordyceps, with IC50 values ranging from 60.8 317.2. The present study provides scientific supporting information for the ethnopharmacological use of Cordyceps militaris as an anticancer agent.
액체배양된 P aecilomyces japonica로부터 cordycepin 분리를 위한 분획용매의 영향
하시영 ( Si Young Ha ),정지영 ( Ji Young Jung ),박재현 ( Jai Hyun Park ),이동환 ( Dong Hwan Lee ),최지원 ( Ji Won Choi ),양재경 ( Jae-kyung Yang ) 한국목재공학회 2020 한국목재공학회 학술발표논문집 Vol.2020 No.1
Cordycepin is a nucleoside analog (3′-deoxyadenosine), which is the major active constituent of Cordyceps militaris and was first reported as a metabolite isolated from a culture broth of P. japonica. To date, cordycepin has been demonstrated to have many pharmacological activities, such as anti-tumor, anti-metastatic immunomodulatory and anti-inflammatory activities, and supply of a large amount of purified cordycepin is essential to the developments of its novel pharmacological use as well as its clinical trials. However, there are very few reports on commercially feasible procedures for isolation and purification of cordycepin. From the discovery of corycepin to the present, the purification methods have been based on column chromatography. The present study was carried out to develop an extraction strategy for cordycepin and to isolation of cordycepin from Paecilomyces japonica in submerged culture. Solvent-solvent extraction method was used to extract cordycepin from submerged culture of P. japonica. Crude concentrated extract of fermented broth was sequentially partitioned with hexane, chloroform and n-butanol. Further we tried to isolation of cordycepin from P. japonica using Sephadex-LH20. As a result, it was confirmed that cordycepin was detected when the liquid culture medium was fractionated using n-butanol. It was also confirmed that a single cordycepin was detected when separated by sephadex column chromatography using 80% methanol as a solvent. At this time, the detection of cordycepin was analyzed by HPLC. It is hoped that these results will be used as basic data for cordycepin separation from Cordyceps mycelia.