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        귀리 원형질체에서 피토크롬 작용에 의한 이노시톨 인지질 대사

        채쾌,표택물,박문환,조태주 ( Quae Chae,Taeck Yul Pyo,Moon Hwan Park,Tae Ju Cho ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2

        The stimulation of breakdown of phosphatidylinositol 4,5-bisphosphate (PIP₂) by the irradiation of red light (660 nm) was investigated by TLC/autoradiography after incorporation of ^(32)Pi into the oat protoplasts. The highest breakdown of PIP₂ was observed at 5 seconds of incubation after irradiation of red light for 3 seconds. When various wavelengths of light were irradiated, the highest breakdown of PIP₂ was observed at 660 nm which is the absorption maximum of phytochrome Pr form. Inositol 1,4,5-trisphosphate (IP₃) which is a metabolic product of PIP₂ was identified in oat cell by Dowex anion column chromatography, using [³H]-IP₃ as a standard compound. The level of IP₃ was substantially increased by irradiation of red light for 3 seconds. In addition, transient changes of the cytosolic free Ca^(2+) concentration by the red light were also monitored with the fluorescent Ca^(2+) indicator Quin 2 in the presence and the absence of extracellular Ca^(2+). The results suggest that signal transduction via IP₃ and Ca^(2+) may also be feasible in the phytochrome mediated cell responses.

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