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- 저자 : 최상돈 ( Chung No Joo (검색결과 2 건)
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쇠의 뇌 미토콘드리아 Aldehyde Dehydrogenase 의 정제와 특성
최상돈,주충노 ( Sang Don Choi,Chung No Joo ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3
Bovine cerebral mitochondrial aldehyde dehydrogenase (ALDH) isozymes were partially purified by ammonium sulfate precipitation, DEAF-Sephacel, Sephacryl S-300, 5`-AMP Sepharose 4B chromatography. They are membrane bound ALDH, soluble high K_m ALDH and low K_m, ALDH. Molecular weights of the native membrane bound ALDH and the soluble high K_m ALDH were 250,000 dalton and 230,000 dalton, respectively and both were found to consist of homologous tetramer by SDS-PAGE analysis and their pI values were 6.52 and 6.22, respectively. The K_m, values for aliphatic aldehydes and aromatic biogenic aldehydes of the membrane bound ALDH were 10^(-3) M level and 10^(-4) M level, respectively and those of the soluble high K_m ALDH were 10^(-4) M level for both aliphatic and aromatic aldehydes. However those of the soluble low K_m ALDH for both aliphatic and aromatic aldehydes were 10^(-6) M level. These results suggest that the mitochondrial membrane bound ALDH may act as barrier when the aldehyde concentration in cytosol is high but when the soluble ALDH, particularly low K_m ALDH may oxidize aldehydes that appeared in mitochondria when the concentration of aldehyde in cytosol was extremely low. It was also found that the membrane ALDH was glycoprotein and NAD^+ dependent while the soluble high K_m ALDH can use either NAD^+ or NADP^+ as a cofactor.
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동물체내에서의 황 ( S ) 대사에 관한 연구 - 쥐 장에서의 흡수와 분포 -
주충노,최상돈 ( Chung No Joo,Sang Don Choi ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
Absorption pattern of solid sulfur through rat intestine using reversed small and large intestinal preparations was investigated and found that no solid sulfur could be absorbed. However, when colonic materials and solid sulfur were added to Erlenmeyer flask containing nutrient broth and incubated, sulfides (H₂S, CH₃SH etc.) were detected in both gaseous phase and medium fraction. We also realized that the H₂S absorbed by colon was partly converted to CH₃SH. Analysis of ^(35)S in the liver after oral administration of solid sulfur containing ^(35)S showed that 19.7% was in the form of SO₄^(2-) and 13.2% was as proteins. Radio-activity distribution of ^(35)S into liver, blood, urine and feces at 24 h after the ^(35)S administration was 0.26%, 0.1%, 41.96%, and 7.4% respectively suggesting that relatively large amounts of sulfur were absorbed but quickly metabolized and excreted in the urine.
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