http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 차주형(Ju Hyoung Cha) (검색결과 4 건)
- 무료
- 기관 내 무료
- 유료
-
-
1-Methylcyclopropene 처리가 머스크멜론 과실의 에틸렌 발생과 품질에 미치는 영향
차주형(Ju Hyoung Cha),황병호(Byung Ho Hwang),이은지(Eun Ji Lee),이긍표(Gung Pyo Lee),김종기(Jongkee Kim) 한국원예학회 2006 원예과학기술지 Vol.24 No.4
본 연구는 네트멜론 ‘Pearl’ 과실을 공시하여 1μLㆍL⁻¹ 농도로 12시간 1-MCP를 처리한 후 25℃에서 저장하며 에틸렌 생합성과 관련된 유전자들의 발현양상과 과실의 품질변화를 조사하였다. 과실의 경도는 무처리구에서는 처리 96시간 경과 후에 8.4N에서 2.6N으로 급격하게 낮아졌으나, 1-MCP처리구에서는 360시간 경과 후에도 3.9N를 유지하였다. 과실의 색도(Hunter ‘a’, ‘b’ 값)는 무처리구에서는 처리 후 72시간이 경과하면서 과피가 급속히 노랗게 착색되었으나, 1-MCP 처리구에서는 동기간 동안 완만하게 노란색이 증가하였다. 과실의 당도는 무처리구에서 10.1oBrix로 급격히 증가한 다음 그 이후에는 약간 감소한 반면에, 처리구에서는 완만하게 증가한 뒤 9.0oBrix 정도로 유지되다가 이후 7.0oBrix로 감소하였다. 한편, 과실의 에틸렌 발생은 무처리구에서는 96시간 경과 후 몇시간에 걸쳐 에틸렌이 급격히 증가하는 전형적인 climacteric 현상을 보였으나, 처리구에서는 360시간이 지나도록 2μLㆍkg⁻¹ㆍh⁻¹ 이하로 유지되었으며, 일시적인 증가는 나타나지 않았다. 1-MCP처리구에서 ripening 중 에틸렌 생합성에 관련된 유전자인 CM-ACS1과 CM-ACO1의 발현양상은 무처리구에 비해 전체적으로 정량적으로 감소하는 경향이었으며, 동시에 발현이 증가되는 시기도 지연되었다. 그러나 에틸렌 수용단백질인 CM-ETR1과 CM-ERS1은 처리구에서 발현이 다소 지연되는 것을 관찰할 수 있었으나 발현양에서는 큰 차이를 보이지 않았다. 본 연구로부터 1-MCP는 에틸렌 수용체와 결합하여 에틸렌의 자가촉매현상(system 2)를 억제함으로써 멜론과실의 에틸렌 발생과 조직의 연화를 지연시켰다. 한편 과실의 당도도 별로 영향을 받지 않았다. Before the onset of climacteric, mature muskmelon fruit (var. ‘Pearl’) grown and harvested from a plastic house was applied with 1-methylcyclopropene (1-MCP), a gaseous inhibitor of ethylene. And its subsequent molecular metabolic changes including expression of genes involved in biosynthesis and perception of ethylene were monitored during storage at 25℃. Fruit treated with 1.0 μLㆍL⁻¹ of 1-MCP for 12 hours displayed a very slow process in ripening-associated physiological changes, compared to fruit provided with air, served as a control. Only a slight increment in ethylene production, accompanied by a significant delay of tissue softening and of yellow color development in fruit skin was manifested by 1-MCP treatment. However, change of total soluble solids content from the fruit either treated with the inhibitor or air remained fairly constant 4 days after the inhibitor treatment. The application of 1-MCP to the muskmelon fruit reduced the expression of CM-ACS1 and delayed the normal expression of CM-ACO1 when compared to the control. However, expression of genes involved in ethylene reception, CM-ETR1 and CM-ERS1, remained fairly unaffected for 15 days of storage after 1-MCP application. These data obtained in the present study support that 1-MCP blocks ethylene binding to its receptor and thus, inhibiting the action of ethylene, consequently suppressing the autocatalytic expression of CM-ACS1. A relatively small peak in ethylene production displayed by 1-MCP treatment can be ascribed to reduced amounts of expression of CM-ACS1 rather than CM-ACO1, lending to inhibition of an autocatalytic production of ethylene, which is a prerequisite for climacteric fruit to undergo ripening.
-
배 과육 조직의 불용성 펙틴의 조성과 효소에 의한 가수분해
김종기(Jongkee Kim),황병호(Byung Ho Hwang),차주형(Ju Hyoung Cha),홍지흔(Ji Heun Hong) 한국원예학회 2008 원예과학기술지 Vol.26 No.3
배 주스 착즙 후 여과 및 저장 중에 생성되는 불용성 펙틴을 분해하는 효소를 분리 동정하고자 본 실험은 수행되었다. ‘신고’ 배 과육 12㎏을 시판중인 pectinase를 처리한 후 용액속의 불용성 펙틴 6.53g을 회수하였다. 이 불용성 펙틴에는 rhamnose/galacturonic acid가 0.19로 rhamnosyl기의 비율이 높았으며, 및 mannose의 함량이 40%에 달하였다. Alginate affinity 칼럼을 사용하여 시판중인 pectinase(Pectinex Ultra SP-L)로부터 polygalcturonase(PG)를 효과적으로 제거하였다. PG가 제거된 Aspergillus pectinase는 불용성 펙틴을 분해하였으며, 분해된 산물은 naringinase의 α-rhamnosidase에 의해 terminal rhamnose를 가진 올리고 당으로 확인되었다. Aspergillus pectinase 에 의해 분해된 펙틴의 분자량의 감소를 gel permeation chromatography로 확인하였으며, 올리고당의 비율은 비교적 낮았다. In order to improve juice quality and filtration process of pear (Pyrus pyrifolia) pulp extracts, insoluble pectins were obtained from pear fruit after enzymatic hydrolysis of the pulp tissue with commercial pectinases. Compositional analysis revealed that the insoluble pectin has a high ratio in rhamnose to galacturonic acid, 0.19, which was a very similar to ‘modified hairy region’ of apple pectin. In addition, the pear pectin was also rich in glucose and mannose, comprising approximately 40% of the polymer. Enzyme (s) capable to degrade the insoluble pectins was isolated from a commercial Aspergillus pecteolytic enzyme preparations using an alginate affinity chromatography. Aspergillus polygalacturonase (PG) was tightly bound to the alginate resin at pH 4.0 and efficiently separated by raising the pH of the elution buffer into 8.0. Gel permeation chromatography of the hydrolysates of insoluble pectin by the enzyme fractions unbound to the alginate resin showed a molecular weight shift from a large to a smaller weight polymers. The enzyme product, insoluble by PG, was further incubated with a α-rhamnosidase of commercial naringinase to hydrolyse terminal rhamnose. An increase in soluble, monomeric rhamnose confirmed that the fungal pectinase had an activity of hydrolyzing rhamno-galacturonosyl backbone of pectin in pear fruit. From the results obtained, it was concluded that insoluble pectins from pear fruit juice was effectively degraded by a fungal pectinase, generating oligosaccharides rich in terminal rhamnose.
-
안영수 ( Young-su An ),백영수 ( Young-su Back ),진송한 ( Song-han Jin ),장충식 ( Choong-sik Jang ),강명희 ( Myoung-hee Kang ),차봉진 ( Bong-jin Cha ),조윤형 ( Youn-hyoung Cho ),차주형 ( Ju-hyeng Cha ),김보연 ( Bo-yeon Kim ) 한국수산해양기술학회(구 한국어업기술학회) 2019 수산해양기술연구 Vol.55 No.1
This study was conducted in order to improve opening efficiency of the miniaturized small-scale net for anchovy boat seine gear to reduce the fleet size. Field experiment was performed to observe geometry of nets by catcher boats. When the distance between the two ships was 150, 300 and 450 m and the speed of towing nets was 0.6, 0.9, and 1.2 kt, the vertical opening and actual opening of each part of the miniaturized small-scale net was as follows: the front part of the wing net, 6.8-9.5 m, 45-63%; the middle part of the wing net, 16.1-30.7 m, 34-65%; the entrance of the inside wing net, 21.6-41.2 m, 44-84%; the square and bosom, 17.4-34.0 m, 38-75%; the entrance of the body net, 16.5-29.4 m, 36-64%; the entrance of the bag net, 14.5-21.9 m, 70-106%; the flapper, 6.7-7.7 m, 81-83%, and the end of the bag net, 8.6-10.9 m, 64-81%. The tension of towing nets was measured to be 2,734-6,812 kg approximately, which indicates that the fleet can tow nets with 350 hp, the standard engine horse power. The fishing operation time was shortened comparing to existent net with the large-scale buoy attachment operation. It was also possible to operate the ship without fish detecting boat.
ScienceON연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
