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- 저자 : 차우양 ( Woo Yang Cha ) (검색결과 2 건)
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Parathion을 경구투여(經口投與)한 Mouse의 체내(體內) Cholinesterase 활성도(活性度) 및 Glucose함량(含量) 조사(調査)
도재철 ( Jae Cheul Do ),이창우 ( Chang Woo Lee ),차우양 ( Woo Yang Cha ),손재권 ( Jae Kweon Son ),정종식 ( Jong Sik Chung ) 한국가축위생학회 1992 韓國家畜衛生學會誌 Vol.15 No.1
The insecticide p-nitropheny diethyl thiophospate is alse known by the symbol E.605 and a legion of trade names including "parathion". The insecticide is widely used in agriculture, but it is highly toxic and now clear that paration behaves like a cholinergic drug by inhibiting the enzyme cholinesterase. In order to know acute toxicity and the changes of glucose concentrations and activity according to time lapsed in female mice given orally single with the half dose to LD50 of parathion, glucose contents and cholinesterase activities in serum as well as cholinesterase activities in whole brain and spinal cord were investigated, otherwise median lethal dose (LD50) of parathion given orally against female mice was determined. The results obtained were summerized as follows; 1. LD50 value of parathion given orally to female mice was 7.lmg/kg(95% confidence limits, 3.8-13.1mg/kg). 2. The inhibition rate of cholinesterase activities in serum of parathion-administrated mice according to time lapsed were peakly decreased to 61% after 30 minutes in comparison to control group, but activities were completely recovered after 48 hours. 3. The inhibition rate of cholinesterase activities in whole brain of parathion- administrated mice according to time lapsed were peakly decreased to 49% after 2 hours in completely recovered after 24 hours. 4. The inhibition rate of cholinesterase activities in spinal cord of parathion- administrated mice according to time lapsed were peakly decreased to 57% after 2 hours in comparison to control group, but activities were completely recovered after 48 hours. 5. The changes of glucose contents in serum of parathion-administrated mice according to time lapsed and in directly after death due to parathion poisoning were no significantly difference.
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이준규 ( Jun Gue Lee ),이은미 ( Eun Mi Lee ),차우양 ( Woo Yang Cha ),김정화 ( Jung Hwa Kim ),김영환 ( Young Hoan Kim ),이양수 ( Yaung Su Lee ),김우현 ( Woo Hyun Kim ),정종식 ( Jong Sik Jyeong ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.1
The present study was conducted to investigate results of B. anthracis isolated from Anthrax in the Kyong-Ju of Feb. 12. 1994. 1. In biochemical feature, B. anthracis was a gram-positive rod, non-motility, sporulation, capsulation. It was positive in gelatinase, starch hydrolysis, glucose. But negative in urease, arabinose, mannitol, xylose. 2. B. anthracis grew well on BA, Br A, TSA after incubation for 24 hours. The organisim grew well on BA, Br. A, NA, TSA after incubation for 72 hours. The media grew well on Br A instead of BA. 3. On 5% blood agar by laboratory animal, β-hemolysis was produced from 36 hours to 48 hours incubation. There was perfect β-hemolysis after incubation for 48 hours. On the other side β-hemolysis was begun on 5% goat blood agar after incubation for 60 hours. 4. In the test of antimicrobial susceptibility, B. anthracis was very sensitive to AM, CF, TE, ENR, GM, AN, DFX, S, P, TYLO, N, KM, C, E, Lins+Sp, NN, CC, CFP, CB were sensitive one by one. B. anthracis was no-sensitive to L, XNL, TIA, CL, SXT. 5. B. anthracis had never sensitivity to direct inoculation of rat and chicken, after subcutanous inj. It was very sensitive to mouse and goat, hamster, guinea pig, rabbit had a sensibility one by one. 6. The dead laboratory animal which had been inoculated with B. anthracis preserved at 37℃ incubation, B. anthracis didn`t cultivate on non-dissected animal after 80 hours but cultivate on dissected animal after 360 hours. 7. The rapidly death could cause high concentration, died from 420 after S.C. 8. The blood smeared samples of hamster from inoculation with B. anthracis, spore germinated in 37℃ after 5 hours, in 32℃ after 6 hours, in room temperature after 9 hours, in -4℃ to -20℃ after 10 hours. 9. B. anthracis inoculated to laboratory animal after SC or PO. Mice and rats feces didn`t cultivated with B. anthracis after SC, but did cultivated with B. anthracis after PO. 10. In the test of disinfectant, B. anthracis was high effective to HgCl2, formalin, effect phenol, cresol, but non-effect NaOH, ethanol.
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