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생화학적 마커와 Microsatellite 마커를 이용한 KCR 랫드의 유전모니터링
박한진(Han-Jin Park),이철호(Chul-Ho Lee),차달선(Dal-Seon Cha),조재우(Jae-Woo Cho),조규혁(Kyu-Hyuk Cho),최양규(Yang-Kyu Choi),현병화(Byung-Hwa Hyun),조진원(Jin-Won Cho),한상섭(Sang-Seop Han),송창우(Chang-Woo Song) 한국실험동물학회 2004 Laboratory Animal Research Vol.20 No.2
The cataract mutant rat which showed lens opacity was found in the colony of Sprague-Dawley rat at Korea Research Institute of Chemical Technology (KRICT) in 1996. The mode of inheritance of affected rats was autosomal recessive. These cataractous rats were mated by sister and brother to 27th (F₂₇) generation and named as Korea Cataract Rat (KCR). We performed genetic monitoring of KCR rat using biochemical and microsatellite markers to determine their genetic identified homozygous and distinctive genetic profile. In biochemical monitoring, six rats from each of the F₁₈/F₁₉F₂₀ were tested using 13 biochemical markers. In molecular genetic monitoring, nine rats from the F₂₅ and F₂₆ were tested using 13 microsatellite markers. No differences were found among the genetic profiles of the F₁₈/F₁₉F₂₀ in biochemical monitoring. Also, in the molecular genetic monitoring, we identified that both genetic profiles of F₂₅ and F₂₆ were the same. The genetic profile of KCR rats was unique against the other major rat strains. In addition, the polymorphysm of KCR rat for microsatellite markers was different from the other strains. These results suggest that KCR rat is genetically well qualified as far as in this test and it has been well established as a new inbred strain which has a novel genetic profile and cataract trait.