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        Acanthamoeba culbertsoni 에서 세포독성 물질의 분리 및 가수분해효소 활성도

        김태우,조영동 ( Tae Ue Kim,Young Dong Cho ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.3

        The cytolytic substance of the pathogenic free living amoeba, Acanthamoeba culbertsoni, has been claimed to be closely related to meningoencephalitis. To characterize and reveal properties of the cytolytic substance, the following methods were used. For cytolytic assay of the cytolytic substance, a chinese hamster ovary cell line was used. The isolation and purification of cytolytic substance involves ultrasonication, ultracentrifugation, ammonium sulfate fractionation, and column chromatography such as CM-Trisacryl M, DEAE-Trisacryl M, Ultrogel AcA 54, hydroxyapatite and Con. A sepharose 4B. Purification fold and yield was 40 times and 11%. Purity was determined by observing one protein band on gel electrophoresis. Molecular weight and subunit of the cytolytic substance were 33,000 dalton and monomer by gel filteration and SDS PAGE. Although the activities of acid phosphatase, β-N-acetyl glucosaminidase, β-glucosidase, phospholipase C were not detected on the purified cytolytic substance, a kind of serine-type protease activity did appear to be responsible for the cytolytic activity.

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