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RT-PCR 기법을 이용한 분변내 소 코로나바이러스 검출
안재문 ( Jae Moon Ahn ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),조부제 ( Bu Je Cho ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.3
The reverse transcriptase polymerase chain reaction (RT-PCR) was used for the detection of bovine coronavirus (BCV) in fecal samples by using reverse transcriptase and two primers which flanked M gene sequence of 407bp. RT-PCR detected bovine coronavirus specifically, but did not detect mouse hepatitis virus (MHV), transmissible gastroenteritis virus (TGEV), and bovine rotavirus (BRV). The M gene sequences of MHV are homologus to that of BCV, but minor differences exist in the primer regions, preventing annealing of the primers. Detection of BCV using RT-PCR was compared with ELISA and the agreement of BCV detection by RT-PCR and ELISA was 95.3%. RNA detection in positive clinical specimens was significantly better by PCR than immunological detection of BCV by ELISA.
최해연 ( Hae Yean Choi ),박재명 ( Jae Myong Park ),이은정 ( Un Jeong Lee ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),조부제 ( Bu Jae Cho ),정운선 ( Un Sun Chung ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.2
To study the efficacy monoclonal antibody(MAb) against bovine rotavirus(BCV) in treatment of calf diarrhea, the MAb was fed to 166 calves with diarrhea from Chung-buk area. The results were summarized as follows. 1. Among the 1,049 calves investigated, 166(16%) calves were infected with BCV. 2. The monthly rate of BCV infection were higher in October to December compared with other months of the year. 3. Among the 166 calves with diarrhea, 137(83%) calves were recovered. 4. Young calves within 7-day-old were more effective in treatment than other ages and the rate of treatment was 86%. 5. Most effective period for treatment of rotavirus was at the first stage of infection.