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감자 ( Solanum tuberosum ) 의 기내 소괴경 형성 단계에 따른 Peroxidase 와 Esterase 동위효소의 양상 변화
정현숙(Hyeon Sook Cheong),송규상(Kyu Sang Song),박진수(Gene Su Park),김성준(Sung Jun Kim),양영기(Young Ki Yang),표병식(Byoung Sik Pyo),황백(Baik Hwang),강영희(Young Hee Kang) 한국식물학회 1993 Journal of Plant Biology Vol.36 No.1
The microtuber was efficiently formed on SH medium containing 9% sucrose from the in vitro propagated shoot of potato (Solanum tuberosum cv. Sumi). In order to investigate gene expression depending on the developmental stage of microtuber, we examined the changes of peroxidase and esterase activities, and their isozyme patterns as well. Peroxidase and esterase activities were the highest at the 7 day-culture of the microtuber and subsequently decreased on the stage of microtuberization, whereas esterase activity increased at the stage of 60 day-culture. However, their activities in the ordinary tuber were higher than those of 60 day-cultured microtuber. In addition, in the peroxidase isozyme pattern two new bands of pI 7.05 and pI 4.65 were appeared at the 15- day and 60 day-cultures, respectively, as shown by isoelectric focusing. Various bands in the esterase isozyme pattern were shown at the 7 day-culture, and the band patterns were a large difference, comparing those of shoot and tuber. New bands in the esterase isozyme pattern also appeared at the 15 day- (pI 4.52) and 60 day-cultures (pI 4.48). These results suggest that the changes of peroxidase and esterase activities and isozyme patterns are an important factor in the differentiation and development of potato.
Nostoc muscorum 과 식물배양세포의 공생유도에 관한 연구 - 2. N. muscorum 과 담배배양세포의 혼합배양에 따른 질소고정능과 형태적 변화
정현숙(Hyeon Sook Cheong),황백(Paik Hwang),강영희(Young Hee Kang) 한국식물학회 1987 Journal of Plant Biology Vol.30 No.4
Investigation on the ability of nitrogen usage by N_2-fixing Nostoc muscorum and cultured tobacco cells when they were associately cultured on nitrogen-free media was carried out. Also, effect of polyamines on the associated cultured condition was carried out. In addition, morphological changes of N. muscorum and cultured tobacco cells in associate culture were observed to detect the possibility of induction of nitrogen fixing ability on cultured plant cells. The activity of nitrogenase increased markedly when N. muscorum was grown exclusively on nitrogen-free media. When N. muscorum was cultured associately with cultured tobacco cells on nitrogen-free media. When N. muscorum was cultured associately with cultured tobacco cells on nitrogen-free media containing polyamines, high activity was detected in 10^-4 M spermine treated group. Investigation on the change of polyamine amounts showed two times increase in spermidine and eight times increase in spermine on a associate culture. These effects of associated culture were shown through morphological change such as dense loclization of N. muscorum around the cultured tobacco cells as well as inside the cells. These results indicate the viability of N. muscorum in cultured tobacco cells and possible induction of nitrogen fixation ability by symbiosis.
정현숙(Hyeon Sook Cheong),김영희(Young Hee Kim),표병식(Byoung Sik Pyo),김성준(Sung Jun Kim),강영희(Young Hee Kang) 한국식물학회 1991 Journal of Plant Biology Vol.34 No.4
The hairy root was induced from potato tuber disc by infection of A. rhizogenes. The detection of the agropine and mannopine by paper electrophoresis confirmed that induced hairy root was transformed by A. rhizogenes. The activity of peroxidase was the highest at 5 weeks and isozyme pattern of peroxidase revealed 3 cathodic bands and 2 anodic bands and new C_4 band (pI 4.6) was observed at 7 weeks after cultivation in hairy root with isoelectric focusing. To study the effect of Ca^2+ on cell wall formation in hairy root, channel blocker of Ca^2+ was treated. The activity of β-glucan synthetase Ⅱ (GS Ⅱ) related to cell wall synthesis was inhibited by about 50% in diltiazem and flunarizine treatment than that of control, but stimulated in CaCl_2 treatment. Therefore these results showed that Ca^2+ might be and effective factor in the cell wall formation. The activity of GS Ⅱ by NaF treatment was increased by about 30%. This result suggested that the activity of GS Ⅱ is changed through phosphorylation process.
고추 ( Capsicum annuum L. ) 의 재분화에 따른 Peroxidase 와 Esterase 동위효소의 양상 변화
정현숙(Hyeon Sook Cheong),임선희(Sun Hee Lim),김영희(Young Hee Kim),강영희(Young Hee Kang) 한국식물학회 1992 Journal of Plant Biology Vol.35 No.1
The plantlet was regenerated on MS medium containing BAP (2 ㎎/ℓ) and IAA (1 ㎎/ℓ) from leaf discs of pepper after 3 weeks of culture. And then, we investigated the activity of peroxidase and esterase and the pattern of their isozymes from leaf, stem and root in order to observe physiological and biochemical changes of the devlopemental stage, respectively. The peroxidase was expressed with tissue specificity because peroxidase activity according to the developemental stage of the tissue was not only highest in the leaf of the pepper at 10 days after it germinated but also 2 new bands of its isozyme were found in pI 7.2 and pI 5.2. However, a new pI 3.4 band was found in the leaf and root of the pepper after 14 days of germination, and in the stem was found out pI 5.2 band. As regeneration of leaf dises was progressed, its peroxiase activity was increased about 80% more than that of control after 14 days of culture and new pI 3.2 and 6.5 bands of it isozyme were found. The results suggested that peroxidase would be connected with regeneration of pepper. Also, esterase activity was increased about 50% more than that of control after 14 days of culture, the pattern of esterase isozyme was shown to be 3 cathodic bands and 1 anodic band after 7 days of culture.
Nostoc muscorum 과 혼합배양한 담배 배양세포의 아미노산 함량 변화
정현숙(Hyeon Sook Cheong),강영희(Young Hee Kang) 한국식물학회 1991 Journal of Plant Biology Vol.34 No.1
Induction of symbiosis between N. muscorum and cultured tobacco cells associately cultured on nitrogen-free medium and effects of polyamines on culture condition were carried out. Analysis of amino acid composition in associate cultures showed increase in total amino acid amounts than single culture of cultured tobacco cells and methionine was markly increased in associative culture on N-free medium treated with 1 mM spermine. These results indicated that the composition of amino acids increased effectively in associative cultures by nitrogen fixation of N. muscorum.
난과식물의 형질전환 유도 및 다량증식에 관한 연구 - 1. 자란 ( Bletilla striata ) 의 미성숙 종자로부터 체세포배 형성 및 식물체 재분화
이정석(Jhung Seok Lee),김영준(Young Jun Kim),정현숙(Hyeon Sook Cheong),황백(Baik Hwang) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.4
Our study was carried out for plant regeneration via somatic embryogenesis from immature seeds of Bletilla striata. The highest frequency of embryogenic callus formation was obtained from the immature seeds (at 150 days after pollination) cultured on Hyponex and VW medium supplemented with 3 ㎎/ℓ 2,4-dichlorophenoxyacetic acid (2,4-D) and 1 ㎎/ℓ kinetin under the dark condition. Multiple somatic embryos were induced when embryogenic callus was transferred to VW medium without growth regulators under continued illumination. Somatic embryos were observed histologically with scanning electron microscopy. Regeneration of Bletilla striata was obtained from somatic embryos with a well-defined scutellum and coleoptile as well as with one or more shoot primordia and root primordia. We think that these methods for orchid multiplication must be useful to access clonal propagation of orchids.