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양성준(Seong Jun Yang),김영옥(Young Ok Kim),손경훈(Kyung Hun Son),이정표,정래석(Rae Sok Jung),양원준(Won Jun Yang),백옥진(Ock Jin Paek),이현경(Hyun Kyung Lee),최상숙(Sang Sook Choi) 대한약학회 2002 약학회지 Vol.46 No.4
A high-performance liquid chromatographic method for the simultaneous quantitative analysis of methylparaben (MP), ethylparaben (EP), propylparaben (PP), butylparaben (BP) and imidazolidinyl urea(IU) or diazolidinyl urea(DU) in cosmetics was studied by using a cyano-propyl column and 0.05M hexanesulfonic acid at 228 nm. Calibration curves were found to be linear in the 60-1000 μg/Ml range (parabens),100-1,250 μg/Ml range (IU) and the 120-2000μg/Ml range (DU). Linear regression analysis of the data demonstrates the efficacy of the method in terms of precision and accuracy. An extraction method is developed and validated in order to apply this chromatographic method to a commercial cosmetic cream. The precision of this method, calculated as the relative standard deviation (RSD) of the recoveries (0.46- 2.71%) was excellent for all compounds.