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흰넓적다리붉은쥐 유래 한타바이러스 분리 및 분자생물학적 특성 비교
송기준(Ki Joon Song),윤형선(Hyung Sun Yoon),고은영(Eun Young Ko),정기모(Gee Mo Jung),박광숙(Gwang Sook Park),이용주(Yong Joo Lee),송진원(Jin Won Song),백락주(Rak Joo Baek) 대한바이러스학회 2000 Journal of Bacteriology and Virology Vol.30 No.1
Two distinct hantaviruses have been isolated from Apodemus agrarius in 1976 and Rattus norvegicus in 1980 in Korea. Since our serosurveys conducted in 1994, a genetically distinct hantavirus from Apodemus peninsulae has been investigated. To isolate hantavirus from A. peninsulae captured in Korea, the lung homogenate of seropositive A. peninsulae inoculated Vero E6 cells. Viral antigen was detected in a progressively higher percentage of cells with subsequent passage after 80 days postinoculation. The new isolate from seropositive Apodemus peninsulae was designated Suchong virus after Suchong valley located in northeastern region of South Korea. Comparing with hantaan virus 76-118 strain, Suchong virus-1, 2, 3 and 4 showed the similarity of 71.0-91.8% at nucleotide and 90.9-94.8% at amino acid sequences in 231 nucleotides region of M segment, and the similarity of 75.1-81.0% at nucleotide and 97.5-100% at amino acid sequences in 237 nucleotides of S segment.
한국 산악지역에서 채집한 야생들쥐의 한타바이러스 감염에 대한 혈청역학적 연구
송진원(Jin Won Song),송기준(Ki Joon Song),백락주(Luck Ju Baek),이용주(Yong Ju Lee),정기모(Ki Mo Jung),고은영(Eun Young Go),김광섭(Kwang Seop Kim),박광숙(Kwang Sook Park) 대한바이러스학회 1999 Journal of Bacteriology and Virology Vol.29 No.1
Hantaan virus is widely distributed among rodent populations in Korea. Two antigenically distinct hantaviruses were isolated from Apodemus agrarius in 1976 and Rattus norvegicus in 1980 in Korea. This study was designed to investigate the serological evidence of hantavirus infection among indegenous wild rodents, which were captured in 11 mountains located in Kyunggi, Kangwon, Chungnan, Chunbug and Kyungnam province of South Korea. A total 252 wild rodents of 3 species were trapped from Myungsung Mt., Chumbong Mt., Kali Mt., Hansuk Mt., Chachil peak, Kyebang Mt., Odae Mt., Kyerong Mt., Kaya Mt., Togju Mt. and Chiri Mt. in 1997. Serologic test for hantavirus infection was performed using hantavirus antigens by indirect immunofluorescent antibody technique. Among 122 Apodemus agrarius, 88 Apodemus peninsulae and 42 Eothenomys regulus; 18 A. agrarius (14.8%), 12 A. peninsulae (13.6%) and 4 E. regulus (9.5%) were immunofluorescent antibody (IFA) positive against hantaan virus. IFA titers 3 Eothenomys regulus sera were higher against puumalavirus than hantaan virus. These data imply that above three species of rodent might be natural reservoirs of hantaviruses in Korea.
강원도 산악지대에서 채집한 야생들쥐의 한타바이러스 감염에 대한 혈청학적 연구
송진원(Jin Won Song),송기준(Ki Joon Song),백락주(Luck Ju Baek),이용주(Yong Ju Lee),정기모(Ki Mo Jung),고은영(Eun Young Go),박광숙(PS Park) 대한바이러스학회 1998 Journal of Bacteriology and Virology Vol.28 No.3
Hantaan virus are widely distributed in rodents populations in Korea. Two antigenically distinct hantaviruses have been isolated from Apodemus agrarius in 1976 and Rattus norvegicus in 1980 in Korea. This study was designed to find the serological evidence of hantavirus infection among indigenous wild rodents captured in 7 Mountains located in Kangwon province of south Korea. A total 191 wild rodents of 3 species were trapped in Chumbong mountain, Kali mountain, Hansuk mounatin, Chachil peak, Bukam ridge, Kyebang mountain and Odae mountain in 1997. Serologic evidence for hantavirus infection were tested using hantavirus antigens by indirect immunofluorescent antibody technique (IFA). Among 85 Apodemus agrarius, 77 Apodemus peninsulae and 29 Eothenomys regulus; 8 A. agrarius (9.4%), 11 A. peninsulae (14.3%) and 4 E. regulus (13.8%) were immunofluorescent antibody positive against hantaan virus. IF antibody titers against Puumala virus of 3 E. regulus sera were higher than against hantaan virus. This data suggest that several antigenically distinct hantaviruses have been circulated in rodent populations in Korea