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RISS 인기검색어
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- 저자 : 전강진 ( Kang Jin Jeon ) (검색결과 2 건)
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복합적인 기계적 자극이 중간엽 줄기세포의 골 분화에 미치는 영향
강윤경 ( Yun Gyeong Kang ),전강진 ( Kang Jin Jeon ),박소희 ( So Hee Park ),신지원 ( Ji Won Shin ),김선연 ( Seon Yeon Kim ),현진숙 ( Jin Sook Hyun ),신정욱 ( Jung Woog Shin ) 한국조직공학·재생의학회 2012 조직공학과 재생의학 Vol.9 No.2s
In this study we investigated the combinational stimulation effects of uniaxial tension and intermittent hydrostatic pressure (IHP) on the differentiation of mesenchymal stem cells (MSCs) into osteoblast without any biochemical agents. For this, we designed a novel bioreactor which can generate not only tensile stimulation and IHP but also combinational stimulation of tension and IHP. We classified experimental groups based on the types of stimulation as follows: (1) NS: no stimulation as a control; (2) IHP: IHP stimulation only (magnitude: 0.1 MPa, intermittent pattern of hydrostatic pressure: 2 min/15 min (on/off), duration: 3 hr/day, for 2 days); (3) TS: tensile stimulation only (magnitude: 3%, frequency: 0.5 Hz, 48 hr in a row); (4) CS: combinational stimulation of (2) and (3). We conducted various biological analyses; DNA contents, ALP activity, Live & Dead staining, F-actin staining, von Kossa staining, and ALP staining. As results, the groups applied mechanical stimulation (IHP, TS, and CS group) showed higher proliferation rate than the group applied no stimulation (NS) at day 8. And amounts of ALP normalized by DNA contents in the groups of TS and IHP were higher than those in CS group at day 5 and day 8, respectively. The highest expressions of ALP and von Kossa staining were observed in IHP group. The expressions of ALP and von Kossa staining in CS were negligible compared with IHP. From this study, we concluded that the single type of mechanical stimulation (IHP or tension) has positive effects but the effect of combinational mechanical stimulation was imperceptible on osteogenesis of MSCs.
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공배양 및 물리적 환경하에서의 중간엽 줄기세포의 혈관 관련 세포로의 분화 경향
박소희 ( So Hee Park ),신지원 ( Ji Won Shin ),김수향 ( Su Hyang Kim ),강윤경 ( Yun Gyeong Kang ),전강진 ( Kang Jin Jeon ),김선연 ( Seon Yeon Kim ),현진숙 ( Jin Sook Hyun ),신정욱 ( Jung Woog Shin ) 한국조직공학·재생의학회 2013 조직공학과 재생의학 Vol.10 No.1s
How to control the differentiation of mesenchymal stem cells (MSCs) into vascular lineage cells have still left much unclear even with numerous studies. Most in vitro studies on the differentiation of stem cells have utilized biochemical reagents. However, recent studies show the potentials of physical and co-culturing environments in control of differentiation. Therefore, we first factored out the in vivo conditions into biochemical reagents, co-culture, and physical stimulation. Then combinational effects of those factors on the differentiation of MSCs into vascular lineage cells were studied. For this EGM (R) -2, co-culture, and shear stress were adopted to meet biochemical, neighboring cells, and physical condition, respectively. A total 4 experimental groups were set depending on co-culture and shear stress. Cell density was 1×104cells/cm2 at all group. For the co-cultures, MSCs were mixed with human umbilical vein endothelial cells (HUVECs) in the ratio of 1:2. The 12 dyne/cm2 of shear stress was engaged: 4 hr/ day for 2 days followed by 48 hr after seeding. For the analyses, the immunofluorescent staining and PCR was performed to evaluate the differentiation of MSCs into vascular lineage cells. The co-culture group without shear stress showed the ECs-related markers even on the first day of the culture. And this was more observable on day 4. However, differentiation of MSCs into smooth muscle cells was found dominant when shear stress was engaged even MSCs were cultured with HUVECs and EGM (R) -2. From this study we confirmed that physical stimulation (12 dyne/ cm2 in our study) plays an important role in controlling differentiation of MSCs into vascular lineage cell types when MSCs were co-cultured with ECs.
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