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시호의 조직배양묘와 배양식물의 종자를 이용한 프러그묘 생산
박철호(Cheol Ho Park),유창연(Chang Yeon Yu),서정식(Jeong Sik Soo),김기식(Ki Sik Kim),박승의(Seung Ue Park),장병호(Byoung Ho Chang) 한국약용작물학회 1995 한국약용작물학회지 Vol.3 No.3
This study was conducted to establish technologies for plug seedling production using somatic embryos-derived regenerants and their seeds in Bupleurum falcatum L. Among distilled water, GA(0.1㎎/ℓ) and putrescine(0.1㎎/ℓ) treated to regenerants for acclimatization, GA was most effective to develop shoots and roots, 1/2X MS medium and NAA 0.1㎎/ℓ+BA 0.5㎎/ℓ enhanced the growth rates of the regenerants and increased dry weight. Activated charcoal effected to grow markedly leaves and roots of the regenerants at the level of 0.4%. Regenerants increased their plant height, root length and dry weight at 30℃. Plug seedlings originated from seeds of the tissue culture regenerants showed the maxium growth on the mixture of peatmoss soil(2) and mountain sand(1). Root length, leaf area and dry weight of plug seedlings increased significantly when No. 1, 2 and 3 of Wondergrow solution were mixed in the ratio of 1.3-0.9-0.1. Light supplement(4%) and high temperature(30℃) promoted the growth of plug seedlings as well as dry weight. Ninety days seedlings were more vigorous and adaptable for transplanting than other seedlings.