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연구보문 : 자연과학; 곤충병원성 균주 Beauveria bassiana 배양액의 이화학적 특성 및 생리활성 검정
이은정 ( Eun Jeong Lee ),장가희 ( Ka Hee Jang ),임선영 ( Seon Yeong Im ),김정준 ( Jeong Jun Kim ),김근 ( Keun Kim ),홍석일 ( Seak Il Hong ),이동진 ( Dong Jin Lee ) 한국국제농업개발학회 2013 韓國國際農業開發學會誌 Vol.25 No.3
The present study was conducted to estimate the antioxidant activities, physicochemical properties and biological activities in Beauveria bassiana. Glucose was the most abundant in B. bassiana (15.2 g?L?1) of 3 day medium by the HPLC analysis of free sugar. Citric acid were observed the highest components in B. bassiana (276.4 mg?L?1) of 3 day medium by the HPLC analysis of organic acid. The composition of Glutamate was the highest among amino acids in B. bassiana (987.0 pmol?μl?1) of 3 day medium. Na was the highest among minerals. It was focused on antioxidant and anticancer activities of B. bassiana. The highest contents of total polyphenol (6.51 ± 0.04 ppm) and flavonoid (1.10 ± 0.06 ppm) were detected in the B. bassiana of 15 day medium. The IC50 value of DPPH radical scavenging activity was ranged from 263.4 ± 13.3 to 461.9 ± 23.9 μg?ml?1 SOD activity was ranged from 93.7 ± 16.6 to 99.8 ± 3.8 μg?ml?1. Also ABTS radical activity was ranged from 99.7 ± 1.2 to 156.9 ± 2.9 μg?ml?1 of which the strongest activity was determined of 15 day medium. Especially, 93.7 ± 16.6 μg?ml?1 of 15 day medium showed highest activity by SOD. In cytotoxicity test against three cancer cell lines, HepG2 (liver), MCF-7 (breast) and A549 (lung), showed the highest toxicity with IC50 values of HepG2 (127.8 ± 2.3 μg?ml?1) cell lines of 10 day medium. Also MCF-7 (129.1 ± 6.8 μg?ml?1), A549 (163.8 ± 4.1 μg?ml?1) showed the highest toxicity of 5 day medium. These data suggest Enthomopathogenic of B. bassiana culture medium could be potential candidate for natural antioxidants.
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소아 및 중증 원형 탈모증 환자에 대한 Cyclosporin-A 도포요법의 치료효과
이찬우 ( Lee Chan Woo ),심승주 ( Sim Seung Ju ),임정준 ( Im Jeong Jun ),이병건 ( Lee Byeong Geon ),송기훈 ( Song Ki Hoon ),김기호 ( Kim Ki Ho ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.4
N/A Background: Prolonged exposure of topical and systemic corticosteroid to skin can result in well-recognized cutaneous abnormalities including cutaneous atrophy, easy bruisibility, increased skin fragility, and increased risk of infection. Skin barrier impairment is also reported as a steroid-induced side effect. A major function of the skin is the formation of a permeability barrier between the external milieu and the organism. Recent studies have shown that chronic corticosteroid negatively impacts epidermal barrier function. As well as this topical corticosteroid not only has antiproliferative actions but also inhibits the difterentiaion of the epidermis, resulting in only has antiproliferative actions but also inhibits the difterentiation of the epidermis, resulting in structural defects in the epidermis. Object: We wanted to determine whether high dose systemic steroid injection would display adverse effects, specifically on; epidermal functions, permeability barrier homeostasis and stratum corneum integrity and cohesion. The basis for such changes was also to be determined. Material and Methods: Systemic steroid was administered by injection each hairless mouse, 8-10 week of age, intraperitoneally with 0.3㎎ triamcinolone acetonide, two times per week for five weeks. For the controlled hairless mice, 0.9% normal saline was administered by the same method of injection. Every week, transepidermal water loss (TEWL) was checked and skin biopsies were taken. Skin specimens were prepared for electron microscopy using both 0.25% ruthenium tetroxide and 4% osmium tetroxide postfixation. For light microscopy staining hematoxylin-eosin and ion capture cytochemistry was used. Results: The results were as follows; 1. From about 1 week onwards, high dose systemic steroid usage produced visible cutaneous changes and significantly increased the TEWL in the group of 0.3 ㎎ triamcinolone acetate injected hairless mice compared with the control. 2. Light microscopic observations of the steroid-injected hairless mice showed gradual thinning of the epidermis from about 2 weeks onwards, compared with the control. Loss of stratum corneum was also observed in the steroid injected hairless mice. 3. The ruthenium tetroxide staining of high dose systemic steroid treated specimens revealed that the lipid bilayer was impaired and fragmented from about 3 weeks. Intercellular spaces were widened and the lipid bilayer either disappeared or showed damage when compared with the control. 4. From about 3weeks onwards. electron microscopic studies revealed, not only a marked decrease in the number of lamellar bodies, but also an abnormal transformation of lamellar bodies in the steroid injected hairless mice compared with the control. 5. Throughout the five weeks, the calcium gradient gradually disappeared in the 0.3㎎ triamcinolone injected hairless mice compared with the control. Consequently, high dose systemic steroid use results in barrier dysfunction and morphological abnormalities. (Korean J Dermatol 2004;42(3):281~289)
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