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생체외 류마토이드 관절염 실험 모델에서 청열사습탕(淸熱瀉濕湯)의 효과
박진상 ( Jin Sang Park ),한명금 ( Myeng Gum Han ),임양의 ( Yang Eui Lim ),이수경 ( Su Kyung Lee ),신병철 ( Byung Cheul Shin ),권영달 ( Young Dal Kwon ),송용선 ( Yung Sun Song ) 한방재활의학과학회 2004 한방재활의학과학회지 Vol.14 No.2
Objective: This study was designed to understand the mechanism of action of Cheongyeolsaseup-tang(Qingrexieshi-tang) (CYSST) leading to control of mast cells on inflammatory condition like RA. Methods: Viability of cells was evaluated by MTT colorimetric assay for 8 h incubation after stimulation of PMA plus A23187. HMC-1 cells (3×105 cells/㎖) were stimulated for 8 hour incubation with PMA plus A23187, we investigated the effect of CYSST on the production of cytokines by activated human mast cell line HMC-1. Results: When CYSST (1 mg/ml) was added, the production of tumor necrosis factor (TNF)-α, interleukin (IL)-6 was significantly inhibited by 37% and 33.6%, respectively in phorbol 12-myristate 13-acetate plus calcium ionophore A23187-stimulated HMC-1 cells. RA is an aggressive inflammatory disease in which chemokines are thought to recruit leukocytes and induce angiogenesis. CYSST also inhibited by 48% the production of IL-8 in HMC-1 cells. In addition, CYSST inhibited the expression of Nuclear factor-κB(NF-κB) in HMC-1 cells. Conclusions: When CYSST was added, the production of TNF-α, IL-6, IL-8 was significantly inhibited. These results imply that CYSST has a critical role on the treatment of the bone damage associated with rheumatoid arthritis.
독활기생탕가미방(獨活寄生湯加味方)이 배양 척수운동신경세포에 미치는 영향
이영보 ( Young Bo Lee ),윤상학 ( Sang Hak Yun ),임양의 ( Yang Eui Lim ),박진상 ( Jin Sang Park ),정택상 ( Taek Sang Joung ),한경호 ( Kyung Ho Han ),이수경 ( Soo Kyung Lee ),신병철 ( Byung Cheul Shin ),권영달 ( Young Dal Kwon ) 한방재활의학과학회 2003 한방재활의학과학회지 Vol.13 No.4
Objectives & Methods : To evaluate the mechanism of oxidative damage by Xanthine oxidase(XO) and hypoxanthine(HX)-induced oxygen radicals, XTT assay was carried out after the cultured mouse spinal motor neurons were preincubated for 5 hours with various concentrations of XO/HX. And DNA synthesis, PKC activity and c-fos immunohistochemistry were measured to evaluate the protective effect of Dokhwalgisaeng-tang(Duhuojisheng-tang: DGT), Dokhwalgisaeng-tanggami(Duhuojisheng-tangjiawei: DGTG), Herbar Chelidoni(HC) water extract on cultured spinal motor neurons damaged by XO/HX. Results : The results were XO/HX showed the quantitative decrease of DNA synthesis, increase of PKC activity and c-fos positive cell, in proportion to concentration and exposed time on cultured spinal motor neurons and DGT, DGTG and HC showed the protective effects against decrease of DNA synthesis and increase of PKC activity and c-fos positive cell by XO/HX. Conclusions : From the above results, it is suggested that XO/HX have a neurotoxic effect on cultured spinal motor neurons and the herbs extract, such as DGT, DGTG and HC, prevent the toxicity of XO/HX effectively.