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미토콘드리아 ribosomal RNA 유전자 염기서열분석에 의한 한국산 연어아과 어류의 유전적 계통도
이희정(Heui Jung Lee),박중연(Jung Youn Park),이정호(Jeong Ho Lee),민광식(Kwang Sik Min),전임기(Im Gi Jeon),유미애(Mi Ae Yoo),이원호(Won Ho Lee) 한국수산과학회 2000 한국수산과학회지 Vol.33 No.2
Complete sequences of the mitochondrial rRNA genes were determined among six salmonines in Korean Waters (Brachymystax lenok, Oncorhynchus keta, O. masou masou, O. masou ishikawae, O. mykiss, and albino mutant of O, mykiss). The purposes of this study were to provide the basic information on levels of mtDNA polymorphism among these species for genetic characterization; discuss phylogenetic relationships among three Oncorhynchus sepecies; demonstrate the utility of rRNA gene sequence data as a genetic marker for distinguishing among Korean salmonines. PCR/direct sequencing data indicated the following consistent results; 1) 12S rRNA genes was 945 bases long in Oncorhynchus species, and 946 bases in B. lenok including one insertion. 2) Of sequence vacation in mitochondrial rRNA regions, transitional substitutions were superior to transversion. 3) The significant differences were not shown in the intraspecific variation values in these gene regions. The percentage sequence divergence values were ranged from 0.066 to 0.212%. 4) The interspecific divergences were greater than the intraspecific variation. Nevertheless, ribosomal RNA genes were more conserved among species than the other mitochondrial genes, and they showed potentiality as an intergenic marker for systematics. In addition, phylogenetic trees, constructed from this data, supported that cherry salmon was closer to chum salmon than to rainbow trout, and that lenok was most distantly related species in six salmonid species.