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브로꼴리 엽록체 ATP Synthase Subunit 1 유전자의 5' Exon 부위 cDNA 클론 및 이의 발현 양상
서미정(Mi Chung Suh),이한길(Han Kuil Yi),홍주봉(Choo Bong Hong) 한국식물학회 1995 Journal of Plant Biology Vol.38 No.2
We isolated a cDNA clone, BLSC1, encoding 5` exon of ATP synthase CF_0 subunit I from broccoli. BLSC1 is 285 nucleotides long which consists of a 5` noncoding region of 34 nucleotides, a 5` exon of 145 nucleotides and an intron of 106 nucleotides. The 5` exon codes for 48 amino acids which reveals mostly hydrophobic. The amino acid sequence deduced from BLSC1 shares 83%, 83% and 91% identities with the genes coding for atpF from wheat, rice and spinach, respectively. Genomic Southern blot analysis for BLSC1 showed a typically strong signal for a gene located in the chloroplast genome. Northern blot analysis identified three major classes of transcripts showing strong positive signals in the leaves, but only trace amounts of the transcripts were identified in the other organs like stems, flower buds and roots.