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이범세 ( Beom Se Lee ),김현경 ( Hyun Kyoung Kim ),김은지 ( Eun Gee Kim ),송운흥 ( Woon Heung Song ) 대한임상병리사협회 1996 임상혈액검사학회 발표자료집 Vol.3 No.1
The therapeutic effect of the anti-coagulative, heparin widely used to care the thromboembolic disorder. In this paper, making use of the aPTT, we study the relation between aPTT and the direct heparin measurement to determine the effective range of the heparin therapy. For the 89 patients under the heparin treatment, the heparin plasma level and aPTT are measured. The conventional therapeutic range fixed for the aPTT has lets of problems. It is effected by such factors which increase for the inflammation as the factor VIII or fibrinogen. Moreover the heparin resistance arises in for the low AT III. We report that the r-value between heparin and aPTT is 0.85, the mean control value of aPTT is 35 see, the therapeutic range is 53~88 see, and its corresponding aPTT ratio is 2.1~3.3 We concluded that it is necessary to set up the effective therapeutic range for the ratio, the heparin measurement and the standardization of aPTT because the therapeutic range of aPTT is effected by several factors.
최명섭 ( Myung Sub Choi ),이현섭 ( Hyunsup Lee ),권혁철 ( Hyuk Chul Kwon ),배문환 ( Moon Hwan Bae ),고영혜 ( Young Hye Ko ),김희진 ( Hee Jin Kim ),이범세 ( Beom Se Lee ),구본경 ( Bon Kyung Koo ) 대한임상검사과학회 2015 대한임상검사과학회지(KJCLS) Vol.47 No.4
A bone marrow biopsy that has undergone decalcification with 10% nitric acid could not be used for various pathological tests and had extremely limited reproducibility. The fixing solution of each experimental group was differentiated in usage, one solution including acid and the other not. The use of the decalcification solution was separated into either acidic or alkaline (EDTA), and further experiments were conducted with differing time phases. When using a fixing solution and decalcification solution which included acid, the specimens were faulty to the extent that all pathological tests were impossible. However specimens that were processed with an EDTA type decalcification solution did not display a non-specific reaction in EBV ISH and were even able to produce results that were at a level suited to various studies or a pathological diagnosis in the FISH, DNA, RNA tests. By improving the fixing and decalcification of bone marrow biopsy, the study was able to make possible ISH, FISH, DNA tests as well as RNA study, and secured the sensitivity, specificity, and reproducibility of various test methods. The stabilization of various test methods that use bone marrow biopsy contributes to the diagnosis, prognosis, prediction, treatment of the patient and provide guidelines for decision-making.