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RISS 인기검색어
- 검색키워드
- 저자 : 윤교복 ( Kyo Bok Yoon ) (검색결과 2 건)
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윤교복 ( Kyo Bok Yoon ),김종술 ( Jong Sool Kim ),정동수 ( Dong Su Chung ),박양주 ( Young Joo Park ),이유섭 ( You Sub Lee ),한정희 ( Jeong Hee Han ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.2
The present study was carried out to investigate the prevalence of brucellosis in Kyungbuk area for the 3 years from 1966 to 1998. Collective milk samples were routinely screened to detect positive farms by using the milk ring test(MRT), and serum agglutination test was performed to detect sero-positive individuals in the MRT positive farms. Attempt were made to isolate the causative organismas from slaughtered sero-positive reactors and some biochemical and polymerase chain reation characters of the isolates were also made to identify the organisms. Seroprevalence to brucellosis in peoples who are close contact with infected dairy herds was also investigated. Brucellosis of dairy cattle was rare before 1997, but has been broken more frequently since early 1998. By the MRT for dairy herds, positive rate was gradually increased every year 0.6% in 1996, 1.5% in 1997, 3.9% in 1998. Among 262 MRT-positive herds, only 21 herds(8.0%) showed positive brucellosis in serological test. The isolation rates of Brucella sp from tested materials were 51.2% in supramammary glands, 39.5% in milks, and 50.0% in pulmonary lymphnode, respectively. Isolated strain and biotype were Brucella(B) arbortus biotype 1 in 26 heads, and were B suis biotype 1 in 2 heads. Isolated strain and vaccine strain were very similar in their colony morphology and staining. In drug susceptibility, isolated stains(B abortus) and vaccine strain(B abortus RB-51) were sensitive to ampicillin, gentamycin, kanamycin, neomycin, penicillin, streptomycin, and tetracycline, but resistant to erythromycin. In the PCR, field strains reacted to BA and 1S711 primers, and vaccine strain reacted to BA, 1S711, and RB51 primers. In the plate agglutination test of 96 sera of human contacted with animals, serum antibody titer detected 1: 100 in one person, 1 : 200 in one, and below 1 : 25 in the others.
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HPLC를 이용한 원유중 잔류 Sulfonamides 분석법 연구
정동수 ( Dong Su Chung ),윤교복 ( Kyo Bok Yoon ),김종술 ( Jong Sool Kim ),신명균 ( Myung Kyun Shin ),김교승 ( Kyo Seung Kim ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.1
This experiment was carried out to detect the residues of sulfonamides in raw milk. Raw milks which does not contain sulfonamides was collected from one of the farm and fortified with 5 sulfonamides(sulfamerazine, sulfamethazine, sulfamonomethoxine, sulfadimethoxine, sulfaqinoxaline). The sulfonamides in the fortified sample were extracted and detected by High Performance Liquid Chromatography. UV/vis detector was used in this experiment. The results obtained were summarized as follows: 1. Chloroform was good as a extracting solution. 2. 15.5% methanol in PDP as a mobile phase solution was best detective condition for SMR, SMT, SMM. But for SDM and SQN the best condition was 23% methanol. 3. The detectable limits of SMR, SMT, SMM were 2ppb. but SDM and SQN were 20ppb because of delayed retention time and relatively low recovery rate. 4. The peaks of SMR, SMT, SMM and SDM were erected at baseline and the apexes were sharp but SQN was round shape.
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