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Column-Switching HPLC法을 利用한 血淸中의 Ciprofloxacin의 定量法에 관한 硏究
李允中,李惠淑,禹炳虎 성균관대학교 약학연구소 1990 成均藥硏論文集 Vol.2 No.1
Abstracts-A small precolumn(40×2 mm, i.d.) packed with Amberlite XAD-7 facilitates the determination of ciprofloxacin in serum by permitting direct injection of relatively small volume(100㎕) of diluted serum. A pH 7.0 phosphate buffer washes out the serum components and acetonitrile: methanol: 5 mM tetrabutylammonium bromide(pH 2.0) (10 : 5 : 85, v/v) subsequently elutes the drug from the precolumn through the analytical column. This method showed high precision with good sensitivity and speed, and a detection limit of 0.1㎍/㎖. The total analysis time per sample was less than 15 min, and the mean coefficients of variation of intra- and inter-assay were both less than 4.8%. The method has been successfully applied to serum samples from human volunteers receiving oral administration of ciprofloxacin.
2-Bromoacetylnaphthalene을 螢光誘導體化劑로 利用한 Ibuprofen의 HPLC 分析에 관한 硏究
李允中,金容熙,曺正吉,禹炳虎 성균관대학교 약학연구소 1990 成均藥硏論文集 Vol.2 No.1
Abstract-A simple, rapid and high sensitive HPLC method for determination of ibuprofen is investigated. 2-Bromoacetylnaphthalene was used as the pre-column fluoresent derivatizing reagent for high performance liquied chromatography. Ibuprofen was derivatized quantitatively into fluorescent compound by reacting with 2-bromoacetylnaphthalene in the presence of 18-crown-6 ether in acetonitrile. The optimum conditions for the derivatization such as concentration of KOH, 18-crown-6 and 2-bromoacetylnaphthalene, reaction temperature and reaction time were investigated. The structure of ibuprofen derivative was confirmed from IR, mass spectra. The fluorescence properties of ibuprofen derivative were examined. The derivative was separated on a reverse phase column in isocratic elution mode using the secondary mixture of acetonitrile and water as the mobile phase. The effluent was monitored by fluorometer. The calibration plots for the peak area versus concentration of ibuprofen observed to be linear.(r=0.999)