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RISS 인기검색어
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- 저자 : 오미정(Mi-Joung OH) (검색결과 4 건)
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韓國 自生 茶나무의 RAPD-marker에 의한 類緣關係
Mi Joung Oh(吳美貞),Byung Hee Hong(洪丙熹) 한국육종학회 1995 한국육종학회지 Vol.27 No.2
Genetic relationship and variation in Korean native tea(Camellia sinensis L.) were analysed using RAPD-PCR markers. Total 19 primers were used and 116 bands of the amplified DNA bands were generated across all 35 collections. Among the obtained bands, 18 of them were monomorphic(15.5%) and the others appeared to be reliable polymorphic(84.5%) marker. Genetic distances(GD) based on the 116 RAPD bands were used to construct phylogenetic dendrogram based on analysis of band sharing data of amplified markers. GDs of them were ranged from 0.070 to 0.367, and the mean value was 0.155. Local collections from same areas of Beknyon-am and Pyagol showed relatively lower GDs(0.122, 0.102, respectively), which infer genetically less divergent within those areas. Local collections from Sunun-sa area showed higher genetic distance(0.222). In genetic relationship based on complete-linkage clustering, the tea collections were classified into three group, A group(A-1, A-2, A-3), B group(B-1, B-2) and C group. It assumes there is extremely lower genetic difference within group. In genetic evolutionary pattern based on Neighbor-joining clustering, the tea collections with similar variation were divided into A group(A-1, A-2), B group(B-1, B-2) and C group(C-1, C-2).
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오미정(Mi-Joung Oh),최준영(Jun-Young Choi),형남인(Nam-In Hyung) 한국차학회 2004 한국차학회지 Vol.10 No.1
The efficient micropropagation system using shoot cultures of tea plant(Camellia sinensis (L.) O. Kuntze) was established. For shoot multiplication and root formation, effects of plant growth regulators, medium and sucrose were investigated. Among BA and IBA combination treatments, the highest number of shoot was obtained with 2.0mg/L BA and 0.2mb/L IBA. MS medium was more effective on shoot length, shoot number, fresh weight than 1/2MS and WPM. Shoot multiplication was more effective on 30g/L sucrose than 20g/L and 40g/L. The optimal concentration for the rooting of shoot cuttings was 3.0mg/L IBA, in which 88.9% rooting frequency was obtained. The plantlets were transferred to soil mixtures with vermiculite and perlite, and successfully acclimatized.
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차나무의 마디배양에 의한 신초 증식에 영향을 미치는 요인
오미정(Mi-Joung Oh),최준영(Jun-Young Choi),형남인(Nam-In Hyung) 한국차학회 2002 한국차학회지 Vol.8 No.1
To investigate the optimal conditions for shoot multiplication from node culture in tea plant (Camellia sinensis (L.) O. Kuntze), the effects of plant growth regulators, medium and sucrose were tested. Shoot proliferation was largely affected by plant growth regulators. Among plant growth regulator treatments, the best shoot multiplication was obtained at 3.0.mg/L BA and 0.5mg/L IBA combination treatmet, which produced normally elongated 4.6 shoots. In proportional to BA concentration increment, shoot length was reduced. Comparing medium kinds, MS and 1/2MS medium was superior to WPM on shoot number and growth. On sucrose concentration treatments, 30g/L sucrose was effective on shoot number and fresh weight. Consequently, these results showed that shoot multiplication through tea node cultures could be successfully carried out on MS medium supplemented with 3.0mg/L BA. 0.5mg/L IBA, and 30g/L sucrose.
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김주희(Kim, Ju-Hee),임근철(Keun-Cheol Lim),박용구(Young-Goo Park),오미정(Mi-Joung OH),김 철(Cheol Kim) 한국차학회 2002 한국차학회지 Vol.8 No.2
There should be investigated many morphological characteristics for gene conservation of tea plants. Among them they are more important characteristics of new and mature leaves, chemical identities of new leaves, disease and insect resistant, cold tolerance, stem and growth vigorous, flower form, the identity of bud sprout, bud sprout early or late, cutting rate, producted tea quality and taste. Those characteristics of tea plants should be analysed continuously for breeding and preservation of new cultivars.
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