임신란관조직의 조직화학적 연구

서상준(SJ Suh),장윤석(YS Chang) 대한산부인과학회 1979 Obstetrics & Gynecology Science Vol.22 No.3

A histochemical study was performed in order to observe the changes of fallopian tubes during pregnancy. Forty-six cases of normal fallopian tubes were obtained at minilaparotomy for tubal sterilization during pregnancy and cesarean section. Two pieces of the tube, one from the isthmic and the other from the ampullar portion, were used in this study in order to study the differences between them. The results obtained were summarized as follows: 1. The average age and parity of these sterilization cases were 34.3 years and 3.3 respectively. 2. Fat was noted mildly in the cytoplasm of tubal epithelium, but was not present in the stroma and muscle. 3. Glycogen was seen moderately, as granules in the perinuclear portion of the tubal epithelium, but midly in the stroma and muscle. 4. Diastase resistant periodic acid Schiff reactive material markedly scattered over the basement membrane, stroma and muscle tissue. But its amount in the apical parts of the secretory cells in the tubal epithelium was only scanty. 5. Mucin content in the secretory cells of the tubal epithelium was slight in amount, but it was not noted in any other cells of the salpingeal tissue. 6. Alkaline phosphatase activity was identified along the surface of the secretory cells of tubal epithelium in mild degree, but moderately in the capillary and endothelium of the arteriole. 7. Acid phosphatase activity was observed moderately in the whole cytoplasm of the tubal epithelial cells with diffuse and granule forms. But its activity was not identified in any other sites of the tube. 8. Beta-glucuronidase activity was moderately present in the tubal epithelium, but it was mild in the stroma, muscle and blood vessel endothelium. 9. Esterase activity observed only slightly in the apical parts of the tubal epithelium and stroma, and it was not noted in any other sites of the tubal tissue. 10. Any differences in the contents of the above materials and enzymes were not identified between the isthmus and ampulla, and between the early and term

폐경후 난관조직의 조직학적 연구

서상준(SJ Suh),장윤석(YS Chang) 대한산부인과학회 1979 Obstetrics & Gynecology Science Vol.22 No.4

A histochemical study on the postmenopausal fallopian tubes was carried out in order to observe the postmenopausal changes of a few meterials and enzymes and compare with the cyclic changes of the normal fallopian tubes. Nine tubal specimens were obtained from laparotomy cases without any salpingeal disease. Two pieces of the tube, one from the isthmic and the other from the ampullar portion, were used in this study in order to find out any difference between them. The results of this study were summarized as follows: 1. The mean age of these postmenopausal cases was 56.3 years and the most frequent laparotomy indication of these cases was chronic cervicitis with cervical erosion. 2. Fat was identified in slight to mild degree in the cytoplasms of the tubal epithelial cells. And fat content in muscle tissue was slight, but negative in the stroma and blood vessel endothelium. 3. Glycogen was seen as granule forms in the perinuclear region of the tubal epithelium, the content of which was mild. And the stromal and muscular glycogen content was mild, too. 4. Diastase resistant periodic acid Schiff reactive material was scattered mildly in the apical parts of the secretory cells of the tubal epithelium. But the contents in the basement membrane, stroma and muscle tissue were moderate to marked. 5. Mucin was not identified in the salpingeal tissue except slight amount in the apical portion of the epithelium. 6. Alkaline phosphatase activity was identified along the apical portion of the secretory cells in the tubal epithelium. The staining intensity of the above portion was slight, but moderate in the capillary and arteriole endothelium. 7. Acid phosphatase activity was observed in the whole cytoplasm of the tubal epithelial cells with diffuse and granular forms. The enzyme activity of the epithelium was mild in the apical portion and trace in the basal portion. 8. Beta-glucuronidase activity was mild in the apical portion of the tubal epithelium, but trace in the basal portion of it and blood vessel

원발성 질평편상피 세포암의 1예

서상준(SJ Suh),이종현(JH Lee),신면우(MW Shin) 대한산부인과학회 1974 Obstetrics & Gynecology Science Vol.17 No.6

This is a case of primary squqmous cell carcinoma of the vagina which is very rare. We present this case with review of literature.

정상난관조직의 조직화학적 연구

서상준(SJ Suh) 대한산부인과학회 1978 Obstetrics & Gynecology Science Vol.21 No.3

A histochemical study on the normal human fallopian tubes was performed in order to observe the cyclic changes of a few materials and enzymes and compare with the cyclic changes of the endometrium. Seventy four tubal specimens were obtained from tubal sterilization cases by minilaparotomy. Two pieces of the tube, one from the isthmic and one from the ampullar portion, were used in this study in order to find any difference between them. The results of this study were summarized as follows: 1. The mean age of these tubal sterilization cases was 31.7 years and their average parity was 2.9. 2. Fat was present not only diffusely distributed in the cytoplasm, but also in the form of granules, which was most marked in the tubal epithelium of the early luteal phase. 3. Glycogen was seen as granule form in the perinuclear region of the tubal epithelium and the content was most marked in the late follicular phase. The amount of glycogen was decreased after ovulation when the ciliary activity in the tube was known to be increased. 4. Diastase resistant periodic acid Schiff reactive material scattered in the basement membrane, stroma and muscle tissue with marked intensity without any cyclic changes. But the amount in the apical parts of the secretory cells in the tubal epithelium has reached its maximum in the early luteal phase. 5. Mucin content in the secretory cells of tubal epithelium was slight throughout the menstrual cycles without change. But it was not found in any other sites of the tube. 6. Alkaline phosphatase activity was observed along the apical portion of secretory cells. The staining intensity was low in the luteal phase, and moderate in the late follicular phase in which the enzyme activity was maximum. 7. Acid phosphatase activity was observed in the whole cytoplasm of the tubal epithelial cells with diffuse and granule forms. But the enzyme activity of cellular survace was more intense than that of the basal portion. The peak activity was in the early luteal phase. 8. Beta-glucuronidase activity wass

정상임신태반에 있어서의 Alkaline Phosphatase 및 Acid Phosphatases의 활성도

서상준(SJ Suh),장윤석(YS Chang) 대한산부인과학회 1974 Obstetrics & Gynecology Science Vol.17 No.12

50예의 만기임신태반과 25예의 조기임신태반에서 ALPdhk ACP의 활성도를 조직화학적 방 법으로 검색한 결과 다음과 같은 결론을 얻었다. 1) ALP (1) 임신 제 6주에 중등도로 syncytium brush border에 나타난 효소활성은 제 9주경부터 점 차 약화되었다가 제 14주 이후에 다시 나타나나 이때는 syncytium과 cytotrophoblast의 세 포질에서 공히 관찰되며 임신이 진행됨에 따라 더욱 증가하여 만삭시에 그 절정에 도달한 다. (2) 혈관, 융모 및 융모막판의 기질조직, 모든 세포의 핵에서는 만삭 및 조기태반 공히 효소 활성을 발견할 수 없었다. 2) ACP (1) 임신 제6주에 매우 강한 활성도를 모든 trophoblast의 세포질에서 볼 수 있었으며 이는 임신 제10-14주에 그 절정에 도달하고 그 뒤 임신이 지속됨에 따라 조금씩 약회되어 만삭 태반에서는 중등도의 활성을 보였다. (2) 융모 및 융모막판의 기질세포들에서 미약한 활성이 있었고 혈관중 모세혈관은 무활성이 고 보다 큰 혈관중 특히 소동맥내피세포에서는 중등도로 Hofbarer 제포에서는 상당히 강한 활성을 거의 임신 전기간 동안 관찰할 수 있었다. Two hydrolytic enzymes were studied histochemically in 25 early and 50 term human placentas. In alkaline phosphatase, the syncytiotrophoblast has had abundant enzyme activity in the first 6 weeks of pregnancy. The activities decreased until 10-14 weeks, and then gradually increased until it reached the peak at term. The cytotrophoblast, on the other hand, in the first trimester had no enzyme activity but certain of these cells acquired alkaline phosphatase activity in abundance in the second and third trimester. In acid phosphatase, there has been marked enzyme activity in the cytopasms of trophoblast and Hofbauer cells at 6th week and reached their greatest activities during 9-13 weeks of pregnancy. During the second and third trimester their activities in thevilli gradually decreased.

제왕절개술중 아두만출 방식에 따른 자궁 혈관손상의 비교 연구

김홍택(HT Kim),서상준(SJ Suh),심재식(JS Shim),원대은(DE Won) 대한산부인과학회 1983 Obstetrics & Gynecology Science Vol.26 No.10

자궁경부편평상피세포암의 조직화학적 연구

장윤석(YS Chang),서상준(SJ Suh) 대한산부인과학회 1976 Obstetrics & Gynecology Science Vol.19 No.2

The enzyme activities in the squamous cell carcinoma of the uterine cervix were studied histochemically In alkaline phosphatase, the malignant cells and stroma had no enzyme activity, but cervical glands and endothelium of the blood vessel had a little activities. In aicd hophatase, cervical glands blood vessel, and stromal cells had moderate enzyme acitivities but the cancer cell had more marked enzyme activity than the control group. The estrase and beta-glucuronidase activities of control group were negative to slight in the epithelium stroma and cervical gland tissue, but the activities of the cancer tissue increased to modereate degree. And all of the above enzyme activities had no relationship between the menstrual cycles.

두 제대가 상호염전을 일으킨 ( 1 ) 일양막성 쌍태임신

원대은(DE Won),서상준(SJ Suh),김홍택(HT Kim) 대한산부인과학회 1982 Obstetrics & Gynecology Science Vol.25 No.2

쌍태임신중에서도 일양막성 쌍태임신은 매우 드물고, 또 태아사망율이 높아서 주의를 요하며, 분만주으이 바른 진단과 신속한 분만을 필요로 한다. 저자들은 두개의 제대가 서로 염전을 일으켜 한 태아의 자궁내 사망을 초래하고 분만지연과 다른 다른 태아의 인유증으로 제왕절개술을 시행한 일양막성 쌍태임신 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Monoamniotic twin pregnancy is a condition where both twins share a common amniotic sac. Not infrequently, their umbilical cords entwine, resulting in a death of one or both peers. A case with such complications is presented with a review of English literature.

진단적 복강경술의 임상적 연구

장윤석(YS Chang),서상준(SJ Suh) 대한산부인과학회 1977 Obstetrics & Gynecology Science Vol.20 No.8

진단한 복강경술을 시행한 92예를 대상으로 하여 분석한 결과 다음과 같은 결론을 얻었다. 1) 적응증은 원발 및 속발성 불임환자가 51예로 가장 많았고 자궁외 임신이 의심되는 환자가 24예로 그 다음의 순이었다. 2) 자궁난관조경술상 양측난관 폐쇄로 진단된 51예중 26예(51%)에서 난관의 양측 혹은 편측의 색소소통 즉 난관의 소통성이 확인되었다. 3) 자궁외 임신이 의심되는 24예중 8예(33.3%)에서 난관임신이 확인되었고, 10예 (41.7%)는 정상, 3예는 정상임신, 2예는 난소낭종파열, 1예는 만성골반강내염증 및 유착임이 판명되었다. 4) 원발성 무월경 12예중 1예는 부신성기증후군임이 확인되어 cortisone치료를 추천 하였고, 2예는 hormone replacement치료를 추천하였으며 그외에도 골반강내장기를 정확하게 관찰함으로써 진단 및 치료에 큰 도움을 주었다. 5) 골반 동통, 골반내종류등이 의심되는 환자에서 복강경술로 그 원인을 규명할 수 있었다. 6) 이소성 자궁 내장치 2예를 복강경술을 시행하여 제거함으로써 개복수술을 피할 수 있었다. 7) 92예의 진단적 복강경술 시행예에서 특기할 만한 합병증은 없었다. Ninety-two diagnostic laparoscopies have been performed to assess the clinical impression from June, 1974 to March, 1977. Fifty-one patients with a diagnosis of bilateral tubal occlusion on hysterosalpingography subsequently underwent laparoscopy and injection of dye through the cervix into the uterus. Bilateral tubal patency was demonstrated in 13 patients (25.5%), and unilateral patency in another 13 cases (25.5%). In the 24 cases of possible ectopic prenancy, only 8 cases (33.3%) have been proved to be tubal pregnancy. Two ectopic intrauterine devices were removen by laparoscopy without laparotomy, and in the 12 primary amenorrhea patients many useful findings for treatment have been found by this procedure. In the view of poor results from tubal plastic procedures, it is advocated that laparoscopy be carried out before tubal surgery on all patients with bilateral negative salpngograms. And thus the laparoscopy may frequently obviate unnecessary plastic tubal surgery and exploratory laparotomy for possible ectopic pregnancy.

거대 자궁근종의 3예

박세웅(SW Park),신순철(SC Shin),서상준(SJ Suh),김홍규(HK Kim) 대한산부인과학회 1978 Obstetrics & Gynecology Science Vol.21 No.7

과거 2년간 거대하다고 사료되는 3예의 자궁근종을 경험하였기에 문헌고찰과 아울러 보고하는 바이다. Three cases of huge uterine myoma were presented, and a brief review on literature was noticed.