성인형 치주염 환자에서 분리된 연쇄상구균의 약제 내성유형에 관한 연구

목성규,신형식 원광대학교 치의학연구소 1992 圓光齒醫學 Vol.2 No.2

It has been reported that flap surgery and antibiotic therapy might be a conventional methods to eliminate periodontitis, But the appearance of resistance strain might decrease the efficacy of treatment. The purpose of this study is to know the drug resistance of streptococci. 30 species of streptococci were isolated from healthy and disease sites of patients with adult periodontitis and identified by biochemical test. The drug resistants were determined by antibiotic disc and were decided by NCCLS standards. L All species of streptococci isolated from the healthy and disease sites of patients with adult periodontitis were susceptible to Penicillin, Ampicillin, Carbenicillin, Chloramphenicol, Vancomycin, Cephalothin and Cefoxitin. 2. All species of streptococci isolated from healthy sites of patients with adult periodontitis showed resistance to Erythromycin. 3. Streptococci isolated from healthy sites of patients with adult periodontitis were susceptible to Tetracycline. 4. S. sanguis Ⅱ isolated from disease sites of patients with adult periodontitis showed resistance to Gentamycin, Amikacin and Clindamycin. 5. S salivariu isolated from healthy sites of patients with adult periodontitis showed resistance to Amikacin and Clindamycin. These results suggested that the resistance to antibiotics were different according to sampling sites. Further study will be needed to clarify the relation between durg resistance and plasmid.

Prostaglandin과 Dibutyryl cAMP가 조골세포의 활성과 파골세포 형성에 미치는 영향

목성규,신형식 원광대학교 치의학연구소 1996 圓光齒醫學 Vol.6 No.2

To maintain its functional integrity, bone is continuously remodelled by a process involving resorption by osteoclasts and formation by osteoblasts. In order to respond to changes in the physical environment or to trauma with the relevant action, this process is strictly regulated by locally synthesized or systemic fators. Prostaglandin E_2 (PGE_2) is perhaps one of the best studied factors, having been known to affect bone cell function for several decades. PGE_2 has both anabolic and catabolic activities. Excess of PGE_2 has been implicated in a number of pathological states associated with bone loss in a number of chronic inflammatory conditions such as periodontal disease and rheumatoid arthritis. PGE_2 and other arachidonic acid metabolites have been shown to be potent stimulators of osteoclastic bone resorption in organ culture. The anabolic effects of PGE_2 were first noticed when an increase in periosteal woven bone formation was seen after the infusion of PGE_2 into infants in order to prevent closure of the ductus arteriosus. The cellular basis for the catabolic actions of PGE_2 has been well characterized. PGE_2 increases osteoclast recruitment in bone marrow cell cultures. Also PGE_2 has a direct action on osteoclast serving to inhibit activity and can also indirectly activate osteoclast via other cells in the vicinity, presumably osteoblast. The cellular mechanisms for the anabolic actions of PGE_2 are not nearly so well understood. The purpose of this paper was to study the effects of PGE_2 and dibutyl(DB)cAMP on osteoblastic clone MC3T3E1 cells and on the generation of osteoclasts from their precursor cells. The effect of PGE_2 and DBcAMP on the induction of alkaline phosphatase(ALP) was investigated in osteoblastic clone MC3T3E1 cells cultured in medium containing 0.4% fetal bovine serum. PGE_2 and DBcAMP stimulated ALP activity and MTT assay in the cells in a dose-dependent manner at concentrations of 10-500ng/㎖. Cycloheximide, protein synthesis inhibitor, inhibited the stimulative effect of PGE_2 and DBcAMP on ALP activity in the cells. PGE_2 also increased the intracellular cAMP content in a dose-dependent fashion with a maximal effect at 500ng/㎖. The effect of PGE_2 on the generation of osteoclasts was investigated in a coculture system of mouse bone marrow cells with primary osteoblastic cells cultured in media containing 10% fetal bovine serum. After cultures, staining for tartrate-resistant acid phosphatase(TRAP)-marker enzyme of osteoclast was performed. The TRAP(+) multinucleated cells(MNCs), which have 3 or more nuclei, were counted. More TRAP(+) MNCs were formed in coculture system than in control group. PGE_2(10-5,10-6M) stimulated the formation of osteoclast cells from mouse bone marrow cells in culture. PGE_2(10-6M) stimulated the formation of osteoclast cells from mouse bone marrow cells in coculture of osteoblastic clone MC3T3E1 cells This results suggest that PGE, stimulates the differentiation of osteoblasts and generation of osteoclast, and are involved in bone formation, as well as in bone resorption.

Prostaglandin과 Dibutyryl cAMP가 조골세포의 활성과 파골세포 형성에 미치는 영향

목성규,유형근,신형식,Mok, Sung-Kyu,You, Hyung-Keun,Shin, Hyung-Shik 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.2

To maintain its functional integrity, bone is continuously remodelled by a process involving resorption by osteoeclasts and formation by osteoblasts, In order to respond to changes in the physical environment or to trauma with the relevant action, this process is strictly regulated by locally synthesized or systemic fators, Prostaglandin $E_2(PGE_2$) is perhaps one of the best studied factors, having been known to affect bone cell function for several decades.$PGE_2$ has both anabolic and catabolic activities. Excess of $PGE_2$ has been implicated in a number of pathological states associated with bone loss in a number of chronic inflammatory conditions such as periodontal disease and rheumatoid arthritis. $PGE_2$ and other arachidonic acid metabolites have been shown to be potent stimulators of osteoclastic bone resorption in organ culture. The anabolic effects of $PGE_2$ were first noticed when an increase in periosteal woven bone formation was seen after the infusion of $PGE_2$ into infants in order to prevent closure of the ductus arteriosus. The cellular basis for the catabolic actions of $PGE_2$ has been well characterized. $PGE_2$increases osteoclast recruitment in bone marrow cell cultures. Also $PGE_2$ has a direct action on osteoclast serving to inhibit activity and can also indirectly activate osteoclast via other cells in the vicinity, presumably osteoblast. The cellular mechanisms for the anabolic actions of $PGE_2$ are not nearly so well understood. The purpose of this paper was to study the effects of $PGE_2$ and dibutyl(DB)cAMP on osteoblastic clone MC3T3El cells and on the generation of osteoclasts from their precursor cells. The effect of $PGE_2$ and DBcAMP on the induction of alkaline phoaphatase(AlP) was investigated in osteoblastic clone MC3T3El cells cultured in medium containing 0.4% fetal bovine serum. $PGE_2$ and DBcAMP stimulated ALP activity and MTT assay in the cells in a dose-dependent manner at concentrations of lO-SOOng/ml. Cycloheximide, protein synthesis inhibitor, inhibited the stimulative effect of $PGE_2$ and DBcAMP on ALP activity in the cells. $PGE_2$also increased the intracellular cAMP content in a dose-dependent fashion with a maximal effect at 500ng/ml. The effect of $PGE_2$ on the generation of osteoclasts was investigated in a coculture system of mouse bone marrow cells with primary osteoblastic cells cultured in media containing 10% fetal bovine serum.After cultures, staining for tartrate-resistant acid phosphatase(TRAP)-marker enzyme of osteoclast was performed. The TRAP(+) multinucleated cells(MNCs), which have 3 or more nuclei, were counted. More TRAP(+) MNCs were formed in coculture system than in control group. $PGE_2(10^{-5}10^{-6}M)$ stimulated the formation of osteoclast cells from mouse bone marrow cells in culture. $PGE_2(10^{-6}M)$ stimulated the formation of osteoclast cells from mouse bone marrow cells in coculture of osteoblastic clone MC3T3E1 cells This results suggest that $PGE_2$ stimulates the differentiation of osteoblasts and generation of osteoclast, and are involved in bone formation, as well as in bone resorption.

성인형 치주염 환자에서 분리된 연쇄상구균의 약제 내성유형에 관한 연구

목성규,김태경,신형식,Mok, S.G.,Kim, Tae-Gyeong,Sin, Hyeong-Sik 대한치주과학회 1992 Journal of Periodontal & Implant Science Vol.22 No.2

치석 제거술과 치근면활택술후 다근치와 단근치의 치은연하 세균 재군락에 대한 비교연구

백호진,목성규,신형식,Baek, Ho-Jin,Mok, Seong-Kyu,Shin, Hyung-Shik 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.3

The purpose of this study was to assess the recolonization of the subgingival microflora following scaling and root planing on single and multiroot teeth with periodontal pockets which were above 5mm. 7 patients with deep pockets were selected for this study. They had not taken antibiotics for 6 months and no history of dental treatment for 6 months before the study. After initial clinical(plaque index, gingival index, probing pocket depth), microbiological and BANA test were determined, each subject received a single session of scaling and root planing, but they were not received oral hygiene instructions. Clinical indices, microbial parameters and BANA test were reassessed 1, 2, and 4 weeks after treatment. The results were as follows : 1. Plaue index, gingival index and pocket depth were not significantly when compared single root group with multiroot group, both groups were siginficantly reduced at 2weeks in plaque index and 2, 4 weeks in gingival index(P<0.05), probing pocket depth was siginificantly changed at 2, 4weeks in multiroot teeth group and 4 weeks in single root teeth group(P<0.05). 2. Percentage of cocci was significantly increased at 4weeks in single root teeth group(P<0.05), motile rod was significantly changed at 4weeks in both group(P<0.05), spirochetes and nonmotile rods were not significantly changed. 3. BANA test was significantly reduced at 1 and 2 weeks (P<0.05) in single root teeth group, multiroot teeth group was not significantly all weeks. This results were suggested that clinical and microbiological effect following scaling and root planing on periodontal disease.