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최상원,오남희,방정환,정관용,김은영,류비탈리 여수대학교 산업기술지역개발연구소 2000 産業基術硏究所 論文集 Vol.9 No.-
Amino acid mixtures containing waste protein resolved by sodium hydroxide hydorolyzing method were obtained. The composition and concentration of amino acid mixtures were analyzed by high performance liquid chromatography. The disinfectant with tetraminecopper(Ⅱ) sulfate monohydratercu[Cu(NH3)4SO4ㆍH2O] from amino acid mixtures was synthesized. Complexes of synthesized disinfectant(amino acid-copper(Ⅱ) complexes) received by using UV- spectrophotometer were confirmed. Sterilizing power on Escherichia coli, Staphycoccus aureus, Xanthomonas and campestris, Pseudomonas solanacearum was tested and compared to synthesized disinfectant and EG105, used generally. Also, we carried out the experiment of sterilizing ability for abseence of metal ions (Ca2+, PO43-, Mg2+). The sterilizing effect of synthesized disinfectant was better than other being not affected by metal ions (Ca2+, PO43-, Mg2+).
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백서에서 금식으로 인한 스트레스 대응축 활성화의 회복조절기전에서 구강인두로부터 입수되는 다양한 맛 자극의 효과
유상배(Sang-Bae Yoo),이종호(Jong-Ho Lee),류비탈리(Vitaly Ryu),장정원(Jeong-Won Jahng) 대한구강악안면외과학회 2011 대한구강악안면외과학회지 Vol.37 No.3
ntroduction: This study examined the regulatory mechanism underlying the meal-induced changes in the hypothalamic-pituitary-adrenal gland (HPA) axis activity. Materials and Methods: Male Sprague-Dawley rats (250-300 g) were hired for two different experiments as follows; 1) rats received either 8% sucrose or 0.2% saccharin ad libitum after 48 h of food deprivation with the gastric fistula closed (real feeding) or opened (sham feeding). 2). rats received 5 ml of intra-oral infusion with 0.2% saccharin or distilled water after 48 h of food deprivation. One hour after food access, all rats were sacrificed by a transcardiac perfusion with 4% paraformaldehyde. The brains were processed for c-Fos immunohistochemistry and the cardiac blood was collected for the plasma corticosterone assay. Results: Real feedings with sucrose or saccharin and sham feeding saccharin but not sucrose, following food deprivation decreased the plasma corticosterone level. c-Fos expression in the nucleus tractus of solitarius (NTS) of the fasted rats was increased by the consumption of sucrose but not saccharin, regardless of the feeding method. On the other hand, the consumption of sucrose or saccharin with real feeding but not the sham, induced cFos expression in the paraventricular nucleus (PVN) of the fasted rats. The intra-oral infusion with saccharin or water decreased the plasma corticosterone level of the fasted rats. Intra-oral water infusion increased c-Fos expression in both the PVN and NTS, but saccharin only in the NTS in the fasted rats. Conclusion: Neither restoration of the fasting-induced elevation of plasma corticosterone nor the activation of neurons in the PVN and NTS after refeeding requires the palatability of food or the post-ingestive satiety and caloric load. In addition, neuronal activation in the hypothalamic PVN may not be an implication in the restoration of the fasting-induced elevation of the plasma corticosterone by oropharyngeal stimuli of palatable food.
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